anti-Human LAT2 antibody for Immunoprecipitation

Recommended LAT2 Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells Family, Member 2 (LAT2) Antibodies
  • LAB
  • NTAL
  • WBSCR15
  • WBSCR5
  • WSCR5
  • LAT2
  • MGC139435
  • Ntal
  • Wbscr5
  • AW125574
  • Wbscr15
  • linker for activation of T-cells family member 2
  • linker for activation of T cells family, member 2
  • LAT2
  • Lat2
Epitope
AA 91-243, Cytoplasmic Domain
15
14
11
11
11
10
8
8
6
5
5
5
5
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
117
35
24
3
3
3
1
1
1
1
1
Host
Mouse
95
35
14
1
Clonality
Monoclonal
Conjugate
This LAT2 antibody is un-conjugated
8
6
6
3
3
3
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
134
43
31
29
20
16
11
6
5
3
3
2
1
1
Options
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Catalog No. ABIN94439
$ 428.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
20.797794 ABIN94440 FACS ICC IP WB PE Mouse IgG1 AA 91-243, Cytoplasmic Domain Log in to see NAP-07 4
17.797794 ABIN94442 IHC (p) IP WB Mouse IgG2a AA 196-212 Log in to see NAP-03 2
16 ABIN2937601 IF/ICC IHC IP WB Rabbit AA 30-209 Log in to see Polyclonal 0
7 ABIN239823 Inhibition IHC IHC (p) IP WB Mouse IgG2a AA 196-212 Log in to see NAP-03 0
5.5 ABIN120727 FACS IF IP WB Mouse IgG1 AA 91-244 Log in to see NAP-07 3
5.5 ABIN536095 FACS IF IP WB Mouse IgG1 Log in to see NAP-07 3
4 ABIN2469996 IP IHC WB Mouse IgG2a AA 196-212 Log in to see NAP-03 0
4 ABIN1723375 IHC (p) IP WB Mouse IgG2a AA 196-212 Log in to see NAP-03 0
4 ABIN5582404 IHC (p) IP WB Mouse IgG2a Log in to see NAP-03 0
4 ABIN532824 FACS IF IP WB Mouse IgG1 Log in to see NAP-07 0
1 ABIN207049 Inhibition FACS ICC IP WB Mouse IgG1 AA 91-243 Log in to see NAP-07 0
1 ABIN596835 IP WB Mouse IgG1 AA 196-212 Log in to see NAP-03 0
1 ABIN1837743 IP WB Mouse IgG1 AA 91-244 Log in to see NAP-07 0
1 ABIN1837742 IP WB Mouse IgG1 AA 196-212 Log in to see NAP-03 0
1 ABIN120728 IHC (p) IP WB Mouse IgG1 AA 196-212 Log in to see NAP-03 1
1 ABIN2297671 IP WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN2314705 IP WB Mouse IgG1 AA 196-212 Log in to see NAP-03 0
1 ABIN2314698 IP WB Mouse IgG1 AA 91-244 Log in to see NAP-07 0
1 ABIN2314701 IC FACS IP IHC WB Mouse IgG1 AA 91-244 Log in to see 6D620 0
1 ABIN2314711 IP IHC WB Mouse IgG2a AA 196-212 Log in to see NAP-03 0

General

Antigen Linker For Activation of T Cells Family, Member 2 (LAT2) Antibodies
Epitope AA 91-243, Cytoplasmic Domain
(15), (14), (11), (11), (11), (10), (8), (8), (6), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(117), (35), (24), (3), (3), (3), (1), (1), (1), (1), (1)
Host Mouse
(95), (35), (14), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT2 antibody is un-conjugated
(8), (6), (6), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(134), (43), (31), (29), (20), (16), (11), (6), (5), (3), (3), (2), (1), (1)
Pubmed 6 references available
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Product Details anti-LAT2 Antibody

Target Details LAT2 Application Details Handling References for anti-LAT2 antibody (ABIN94439) Images
Specificity The antibody NAP-07 reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
Cross-Reactivity (Details) Mouse, Human, Other not tested
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Immunogen Recombinant cytoplasmic domain (aa 91-243) of human NTAL.
Clone NAP-07
Isotype IgG1
Plasmids, Primers & others

Target Details LAT2

Product Details anti-LAT2 Antibody Application Details Handling References for anti-LAT2 antibody (ABIN94439) Images back to top
Antigen
Alternative Name NTAL / LAB (LAT2 Antibody Abstract)
Background NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Pathways Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT2 Antibody Target Details LAT2 Handling References for anti-LAT2 antibody (ABIN94439) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-LAT2 Antibody Target Details LAT2 Application Details References for anti-LAT2 antibody (ABIN94439) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-LAT2 antibody (ABIN94439)

Product Details anti-LAT2 Antibody Target Details LAT2 Application Details Handling Images back to top
Product cited in:

Duque-Afonso, Yalcin, Berg, Abdelkarim, Heidenreich, Lübbert: "The HDAC class I-specific inhibitor entinostat (MS-275) effectively relieves epigenetic silencing of the LAT2 gene mediated by AML1/ETO." in: Oncogene, Vol. 30, Issue 27, pp. 3062-72, 2011

Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, 2007

Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, 2004

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, 2004

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002

Background publications

Yamasaki, Ishikawa, Sakuma, Kanagawa, Cheng, Malissen, Saito: "LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4406-15, 2007

Images

Product Details anti-LAT2 Antibody Target Details LAT2 Application Details Handling References for anti-LAT2 antibody (ABIN94439) back to top
Supplier Images
Confocal Microscopy (CM) image for anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody (ABIN94439) Confocal microscopy Subcellular localization of NTAL by confocal microscopy in THP-1 ...