anti-Guinea Pig N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 4 antibody for Western Blotting

Recommended N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 4 Antibody (supplied by: Log in to see )

Antigen
N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 4 (NDST4) Antibodies
  • NDST-4
  • NHSST4
  • 4930439H17Rik
  • N-deacetylase and N-sulfotransferase 4
  • N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4
  • NDST4
  • Ndst4
Epitope
Middle Region
10
10
7
4
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
28
5
3
3
3
3
2
1
1
1
Host
Rabbit
25
3
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Western Blotting (WB)
28
15
Options
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Available images

Catalog No. ABIN2783876
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4.8439007 ABIN2783875 WB Rabbit N-Term Log in to see Polyclonal 1

General

Antigen N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 4 (NDST4) Antibodies
Epitope Middle Region
(10), (10), (7), (4), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(28), (5), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(25), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(28), (15)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN980560
Sequence YLFLLMHPSI ISNLPSPKTF EEVQFFNGNN YHKGIDWYMD FFPTPSNTTS
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against NDST4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human NDST4

Target Details

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Antigen
Alternative Name NDST4 (NDST4 Antibody Abstract)
Background NDST4 is an essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. It modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated
Alias Symbols: NDST-4, NHSST4
Protein Size: 872
Molecular Weight 101 kDa
Gene ID 64579
NCBI Accession NM_022569, NP_072091
UniProt Q9H3R1
Pathways Glycosaminoglycan Metabolic Process

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 872 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Aikawa, Grobe, Tsujimoto, Esko: "Multiple isozymes of heparan sulfate/heparin GlcNAc N-deacetylase/GlcN N-sulfotransferase. Structure and activity of the fourth member, NDST4." in: The Journal of biological chemistry, Vol. 276, Issue 8, pp. 5876-82, 2001

Images

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Supplier Images
Western Blotting (WB) image for anti-N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 4 (NDST4) (Middle Region) antibody (ABIN2783876) WB Suggested Anti-NDST4 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Pos...
Western Blotting (WB) image for anti-N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 4 (NDST4) (Middle Region) antibody (ABIN2783876) WB Suggested Anti-NDST4 Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 Posit...