anti-Human Prokineticin Receptor 1 antibody for Immunohistochemistry

Recommended Prokineticin Receptor 1 Antibody (supplied by: Log in to see )

Antigen
Prokineticin Receptor 1 (PROKR1) Antibodies
  • EG-VEGFR1
  • Gpr73
  • Pkr1
  • GPR73
  • GPR73a
  • PKR1
  • ZAQ
  • PROKR1
  • prokineticin receptor 1
  • Prokr1
  • PROKR1
Epitope
N-Term
14
9
5
4
3
2
1
1
1
Reactivity
Human
58
17
14
3
3
2
Host
Rabbit
43
18
Clonality
Polyclonal
Conjugate
This Prokineticin Receptor 1 antibody is un-conjugated
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
31
22
17
17
13
7
7
5
1
1
1
1
1
Supplier
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Catalog No. ABIN1049243
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.673489 ABIN1049242 IHC IHC (p) Rabbit C-Term Log in to see Polyclonal 0
1 ABIN347055 FACS IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 5
1 ABIN4347604 FACS IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN4347603 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN797686 IHC WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN4347601 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 0
1 ABIN4347602 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 0
1 ABIN1103794 FACS IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2341975 IHC Rabbit C-Term Log in to see Polyclonal 0
1 ABIN6065209 ELISA IHC Rabbit IgG AA 1-62 Log in to see Polyclonal 0
1 ABIN2341976 IHC WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN4889445 ELISA FACS IHC WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN6034266 IF/ICC IHC IP WB Rabbit Log in to see Polyclonal 0
1 ABIN6026512 IF/ICC IHC IP WB Mouse Log in to see 0

General

Antigen Prokineticin Receptor 1 (PROKR1) Antibodies
Epitope N-Term
(14), (9), (5), (4), (3), (2), (1), (1), (1)
Reactivity Human
(58), (17), (14), (3), (3), (2)
Host Rabbit
(43), (18)
Clonality
Polyclonal
Conjugate This Prokineticin Receptor 1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(31), (22), (17), (17), (13), (7), (7), (5), (1), (1), (1), (1), (1)
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Product Details anti-Prokineticin Receptor 1 Antibody

Target Details Prokineticin Receptor 1 Application Details Handling Images
Brand IHC-plus™
Specificity Human PROKR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset (93%) Elephant, Bovine (80%).
Purification Immunoaffinity purified
Immunogen Synthetic 15 amino acid peptide from N-terminal extracellular domain of human PROKR1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset (93%), Elephant, Bovine (80%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details Prokineticin Receptor 1

Product Details anti-Prokineticin Receptor 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PROKR1 (PROKR1 Antibody Abstract)
Background Name/Gene ID: PROKR1
Subfamily: Prokineticin
Family: GPCR

Synonyms: PROKR1, G protein-coupled receptor 73, GPCR73A, G protein-coupled receptor ZAQ, G-protein coupled receptor 73, G-protein coupled receptor ZAQ, GPR73, GPR73a, PK-R1, Prokineticin receptor 1, EG-VEGF receptor, PKR1, ZAQ
Gene ID 10887
Pathways Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma

Application Details

Product Details anti-Prokineticin Receptor 1 Antibody Target Details Prokineticin Receptor 1 Handling Images back to top
Application Notes Approved: IHC, IHC-P (7 - 16 μg/mL), WB
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-Prokineticin Receptor 1 Antibody Target Details Prokineticin Receptor 1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-Prokineticin Receptor 1 Antibody Target Details Prokineticin Receptor 1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Prokineticin Receptor 1 (PROKR1) (N-Term) antibody (ABIN1049243) Anti-PROKR1 antibody ABIN1049243 IHC staining of human skeletal muscle. Immunohistoc...