anti-Wild boar (Sus scrofa) BNP antibody for Immunohistochemistry (Frozen Sections)

Recommended BNP Antibody (supplied by: Log in to see )

Antigen
Brain Natriuretic Peptide (BNP) Antibodies
  • bnp
  • BNP1
  • NPPB
  • BNP
  • Bnf
  • AA408272
  • B-type natriuretic peptide
  • natriuretic peptide B
  • natriuretic peptide type B
  • nppb
  • NPPB
  • Nppb
Epitope
AA 26-131
10
5
5
4
4
4
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Wild boar (Sus scrofa), Pig (Porcine)
77
9
7
5
5
2
Host
Rabbit
53
48
1
1
1
Clonality
Polyclonal
Conjugate
This BNP antibody is un-conjugated
10
3
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
67
59
42
25
15
13
7
7
7
5
4
2
1
1
1
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Catalog No. ABIN1077874
$ 378.00
Plus shipping costs $45.00

General

Antigen Brain Natriuretic Peptide (BNP) Antibodies
Epitope AA 26-131
(10), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Wild boar (Sus scrofa), Pig (Porcine)
(77), (9), (7), (5), (5), (2)
Host Rabbit
(53), (48), (1), (1), (1)
Clonality
Polyclonal
Conjugate This BNP antibody is un-conjugated
(10), (3)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(67), (59), (42), (25), (15), (13), (7), (7), (7), (5), (4), (2), (1), (1), (1)
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Product Details anti-BNP Antibody

Target Details BNP Application Details Handling Images
Sequence MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF - HPLGG AGLASELPGI QELLDRLRDR VSELQAERTD LEPLRQDRGL TEAWEAREAA PTGVLGPRSS IFQVLRGIRS PKTMRDSGCF GRRLDRIGSL SGLGCNVLRR Y
Specificity It has been selected for its ability to recognize BNP in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant BNP expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against BNP.
Isotype IgG
Plasmids, Primers & others

Target Details BNP

Product Details anti-BNP Antibody Application Details Handling Images back to top
Antigen
Pathways Hormone Activity

Application Details

Product Details anti-BNP Antibody Target Details BNP Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant BNP (His26~Tyr131) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-BNP Antibody Target Details BNP Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

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Supplier Images
Western Blotting (WB) image for anti-Brain Natriuretic Peptide (BNP) (AA 26-131) antibody (ABIN1077874) anti-Brain Natriuretic Peptide (BNP) (AA 26-131) antibody