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|Antigen||Death Inducer-Obliterator 1 (DIDO1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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|Specificity||This antibody detects endogenous levels of DIDO1 / Dio-1 protein. (region surrounding Ser186)|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse.|
|Purification||Affinity Chromatography using epitope-specific immunogen|
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|Alternative Name||DIDO1 (DIDO1 Antibody Abstract)|
|Background||DIDO1 / Dio-1 (Death Inducer-Obliterator-1) is a putative transcription factor that contains two zinc finger motifs. Dio-1 translocates to the nucleus, and activates apoptosis during limb development. Programmed cell death, a highly regulated form of apoptosis, plays an important role in determining the amount of tissue, the shape, and the definition of each digit during limb development. Dio-1 expression is upregulated when an apoptotic signal is detected, and subsequently apoptosis is induced. This process is similar to the expression of NFκB and NGF in response to external signals. Dio-1 expression is suppressed by caspase inhibitors and Bcl-2 expression. This supports the theory that Dio-1 functions in the onset of programmed cell death.Synonyms: C20orf158, DATF1, Death-inducer obliterator 1, KIAA0333|
|Molecular Weight||approx. 244 kDa|
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
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