anti-Human Death Inducer-Obliterator 1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Death Inducer-Obliterator 1 Antibody (supplied by: Log in to see )

Antigen
Death Inducer-Obliterator 1 (DIDO1) Antibodies
  • BYE1
  • C20orf158
  • DATF-1
  • DATF1
  • DIDO2
  • DIDO3
  • DIO-1
  • DIO1
  • dJ885L7.8
  • 6720461J16Rik
  • C530043I07
  • D130048F08Rik
  • Datf1
  • Dido2
  • Dido3
  • mKIAA0333
  • DIDO1
  • datf1l
  • fi35b04
  • wu:fi35b04
  • zgc:158157
  • DKFZp469J1523
  • bye1
  • dio1
  • datf1
  • dido2
  • dido3
  • dio-1
  • death inducer-obliterator 1
  • death inducer-obliterator 1 L homeolog
  • DIDO1
  • Dido1
  • dido1.L
  • dido1
Reactivity
Human, Mouse (Murine)
60
30
19
8
7
7
7
5
5
4
3
1
1
Host
Rabbit
53
10
Clonality
Polyclonal
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
56
29
20
9
6
5
4
3
2
Supplier
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Catalog No. ABIN498449
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN214094 IHC IHC (p) WB Rabbit IgG AA 72-121 Log in to see Polyclonal 0
7 ABIN5576607 IHC (p) WB Rabbit Log in to see Polyclonal 0
4 ABIN5959421 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2892374 IHC IHC (p) WB Rabbit Ser186 Log in to see Polyclonal 0

General

Antigen Death Inducer-Obliterator 1 (DIDO1) Antibodies
Reactivity Human, Mouse (Murine)
(60), (30), (19), (8), (7), (7), (7), (5), (5), (4), (3), (1), (1)
Host Rabbit
(53), (10)
Clonality
Polyclonal
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(56), (29), (20), (9), (6), (5), (4), (3), (2)
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Product details

Target Details Application Details Handling Images
Specificity This antibody detects endogenous levels of DIDO1 / Dio-1 protein. (region surrounding Ser186)
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Purification Affinity Chromatography using epitope-specific immunogen

Target Details

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Antigen
Alternative Name DIDO1 (DIDO1 Antibody Abstract)
Background DIDO1 / Dio-1 (Death Inducer-Obliterator-1) is a putative transcription factor that contains two zinc finger motifs. Dio-1 translocates to the nucleus, and activates apoptosis during limb development. Programmed cell death, a highly regulated form of apoptosis, plays an important role in determining the amount of tissue, the shape, and the definition of each digit during limb development. Dio-1 expression is upregulated when an apoptotic signal is detected, and subsequently apoptosis is induced. This process is similar to the expression of NFκB and NGF in response to external signals. Dio-1 expression is suppressed by caspase inhibitors and Bcl-2 expression. This supports the theory that Dio-1 functions in the onset of programmed cell death.Synonyms: C20orf158, DATF1, Death-inducer obliterator 1, KIAA0333
Molecular Weight approx. 244 kDa
Gene ID 11083
NCBI Accession NP_071388
UniProt Q9BTC0
Pathways Hormone Activity

Application Details

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Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

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Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

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Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Death Inducer-Obliterator 1 (DIDO1) antibody (ABIN498449) Immunohistochemistry analyzes of paraffin-embedded human colon carcinoma tissue using...
Western Blotting (WB) image for anti-Death Inducer-Obliterator 1 (DIDO1) antibody (ABIN498449) Western blot (WB) analysis with extracts from 293 cells using DIDO1 /  Dio-1 antibody .