anti-Zebrafish (Danio rerio) Death Inducer-Obliterator 1 antibody for Western Blotting

Recommended Death Inducer-Obliterator 1 Antibody (supplied by: Log in to see )

Antigen
Death Inducer-Obliterator 1 (DIDO1) Antibodies
  • BYE1
  • C20orf158
  • DATF-1
  • DATF1
  • DIDO2
  • DIDO3
  • DIO-1
  • DIO1
  • dJ885L7.8
  • 6720461J16Rik
  • C530043I07
  • D130048F08Rik
  • Datf1
  • Dido2
  • Dido3
  • mKIAA0333
  • DIDO1
  • datf1l
  • fi35b04
  • wu:fi35b04
  • zgc:158157
  • DKFZp469J1523
  • bye1
  • dio1
  • datf1
  • dido2
  • dido3
  • dio-1
  • death inducer-obliterator 1
  • death inducer-obliterator 1 L homeolog
  • DIDO1
  • Dido1
  • dido1.L
  • dido1
Epitope
C-Term
16
5
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
60
30
18
7
6
6
6
5
4
4
2
1
1
Host
Rabbit
53
10
Clonality
Polyclonal
Application
Western Blotting (WB)
56
29
20
10
6
6
4
3
2
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Catalog No. ABIN2781180
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0807028 ABIN927824 WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN324532 WB Rabbit IgG AA 480-529 Log in to see Polyclonal 0

General

Antigen Death Inducer-Obliterator 1 (DIDO1) Antibodies
Epitope C-Term
(16), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(60), (30), (18), (7), (6), (6), (6), (5), (4), (4), (2), (1), (1)
Host Rabbit
(53), (10)
Clonality
Polyclonal
Application Western Blotting (WB)
(56), (29), (20), (10), (6), (6), (4), (3), (2)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN975796
Sequence KKAVVVPARS EALGKEAACE SSTPSWASDH NYNAVKPEKT AAPSPSLLYK
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%
Characteristics This is a rabbit polyclonal antibody against DIDO1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human DIDO1

Target Details

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Antigen
Alternative Name DIDO1 (DIDO1 Antibody Abstract)
Background In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. DIDO1 gene is similar to the mouse gene and therefore is thought to be involved in apoptosis.Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms.Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms.
Alias Symbols: BYE1, C20orf158, DATF1, DIDO2, DIDO3, DIO-1, DIO1, DKFZp434P1115, FLJ11265, KIAA0333, MGC16140, dJ885L7.8, DATF-1
Protein Interaction Partner: UBC, WWOX, RPA3, RPA2, RPA1, EED, BMI1, HECW2, SRPK2, KCNK4, ARAF, Dlg4, H2AFY, U2AF1, SON, RPL18A, KRT7, APP, PCDHA2, H2AFY2, GNL3, RRS1, SIRT7, SUMO2, WWP2, WWP1, DVL3, SRSF1, HNRNPK,
Protein Size: 562
Molecular Weight 61 kDa
Gene ID 11083
NCBI Accession NM_022105, NP_071388
UniProt Q9BTC0
Pathways Hormone Activity

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 562 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Trachana, van Wely, Guerrero, Fütterer, Martínez-A: "Dido disruption leads to centrosome amplification and mitotic checkpoint defects compromising chromosome stability." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 8, pp. 2691-6, 2007

Images

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Supplier Images
Western Blotting (WB) image for anti-Death Inducer-Obliterator 1 (DIDO1) (C-Term) antibody (ABIN2781180) WB Suggested Anti-DIDO1 Antibody Titration: 0.2-1 ug/ml Positive Control: Transfec...