anti-Dog (Canine) GATA3 antibody for Enzyme Immunoassay

Recommended GATA3 Antibody (supplied by: Log in to see )

Antigen
GATA Binding Protein 3 (GATA3) Antibodies
  • HDR
  • HDRS
  • Gata-3
  • jal
  • cb550
  • gta3
  • GATA-3
  • XGATA3
  • xgata-3
  • hdr
  • xGATA-3
  • GATA3
  • GATA transcription factor 3
  • T4L20.260
  • T4L20_260
  • gata3
  • GATA binding protein 3
  • GATA binding protein 3 S homeolog
  • GATA transcription factor 3
  • amidase
  • putative amidase
  • GATA-binding protein 3
  • GATA3
  • Gata3
  • gata3
  • gata3.S
  • gatA3
Epitope
N-Term
29
17
15
15
10
10
9
9
8
8
7
7
7
4
3
3
3
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
227
125
48
14
12
9
8
7
7
5
5
4
4
2
1
Host
Goat
129
77
21
8
Clonality
Polyclonal
Conjugate
This GATA3 antibody is un-conjugated
7
7
6
6
5
4
4
4
3
2
2
2
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
175
102
73
41
35
28
27
15
8
5
4
2
1
Supplier
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Catalog No. ABIN5551872
$ 462.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN615854 EIA IHC (p) WB Goat N-Term Log in to see Polyclonal 0

General

Antigen GATA Binding Protein 3 (GATA3) Antibodies
Epitope N-Term
(29), (17), (15), (15), (10), (10), (9), (9), (8), (8), (7), (7), (7), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(227), (125), (48), (14), (12), (9), (8), (7), (7), (5), (5), (4), (4), (2), (1)
Host Goat
(129), (77), (21), (8)
Clonality
Polyclonal
Conjugate This GATA3 antibody is un-conjugated
(7), (7), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(175), (102), (73), (41), (35), (28), (27), (15), (8), (5), (4), (2), (1)
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Product Details anti-GATA3 Antibody

Target Details GATA3 Application Details Handling Images
Sequence EVTADQPRWV SHH-C
Specificity This antibody detects GATA3 at N-term. It is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide from the N Terminus of the protein sequence according to NP_001002295.1, NP_002042.1 . Genename: GATA3
Plasmids, Primers & others

Target Details GATA3

Product Details anti-GATA3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GATA3 (GATA3 Antibody Abstract)
Background The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.Synonyms: GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3
Gene ID 2625
NCBI Accession NP_001002295
UniProt P23771
Pathways Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation

Application Details

Product Details anti-GATA3 Antibody Target Details GATA3 Handling Images back to top
Application Notes Peptide ELISA: Antibody detection limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell line MOLT4(calculated MW of 48.0 according to NP_001002295.1 and 47.0 kDa according toNP_002042.1). Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells inthe interfollicular area and in germinal centres. Recommended concentration, 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GATA3 Antibody Target Details GATA3 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.