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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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12.863943 | ABIN604963 | ELISA IHC IHC (p) WB | Goat | AA 2-14 | Log in to see | Polyclonal | 0 |
General |
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Antigen | GATA Binding Protein 3 (GATA3) Antibodies |
Epitope | AA 36-85 Alternatives |
Reactivity | Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This GATA3 antibody is un-conjugated Alternatives |
Application |
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-GATA3 AntibodyTarget Details GATA3 Application Details Handling Images |
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Brand | IHC-plus™ |
Specificity | Human GATA3 |
Predicted Reactivity | Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Guinea pig (92%) Zebrafish (84%). |
Purification | Immunoaffinity purified |
Immunogen |
Synthetic peptide located between aa36-85 of human GATA3 (P23771, NP_002042). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Zebra finch, Chicken (100%), Bat, Guinea pig, Trout, Salmon (92%), Zebrafish (84%). Type of Immunogen: Synthetic peptide |
Isotype | IgG |
Plasmids, Primers & others | |
Target Details GATA3Product Details anti-GATA3 Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | GATA3 (GATA3 Antibody Abstract) |
Background |
Name/Gene ID: GATA3 Family: Transcription factor Synonyms: GATA3, GATA-binding factor 3, HDRS, GATA binding protein 3, GATA-binding protein 3, HDR |
Gene ID | 2625 |
NCBI Accession | NP_002042 |
UniProt | P23771 |
Pathways | Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation |
Application DetailsProduct Details anti-GATA3 Antibody Target Details GATA3 Handling Images back to top |
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Application Notes |
Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL) Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500. |
Comment |
Target Species of Antibody: Human |
Restrictions | For Research Use only |
HandlingProduct Details anti-GATA3 Antibody Target Details GATA3 Application Details Images back to top |
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Format | Lyophilized |
Reconstitution | Distilled water |
Concentration | Lot specific |
Buffer | Lyophilized from PBS with 2 % sucrose |
Handling Advice | Avoid repeat freeze-thaw cycles. |
Storage | 4 °C,-20 °C |
Storage Comment |
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year) Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles. |
ImagesProduct Details anti-GATA3 Antibody Target Details GATA3 Application Details Handling back to top |
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Supplier Images |