anti-Human GATA3 antibody for Fluorescence Microscopy

Recommended GATA3 Antibody

Antigen
GATA Binding Protein 3 (GATA3) Antibodies
  • HDR
  • HDRS
  • Gata-3
  • jal
  • cb550
  • gta3
  • GATA-3
  • XGATA3
  • xgata-3
  • hdr
  • xGATA-3
  • GATA3
  • GATA transcription factor 3
  • T4L20.260
  • T4L20_260
  • gata3
  • GATA binding protein 3
  • GATA binding protein 3 S homeolog
  • GATA transcription factor 3
  • amidase
  • putative amidase
  • GATA-binding protein 3
  • GATA3
  • Gata3
  • gata3
  • gata3.S
  • gatA3
Epitope
C-Term
43
17
15
15
12
10
9
9
9
8
8
8
7
6
6
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Human
250
129
54
15
13
10
8
7
7
5
5
4
4
2
1
Host
Rabbit
153
76
21
8
Clonality
Polyclonal
Conjugate
This GATA3 antibody is un-conjugated
11
6
6
6
6
6
6
6
5
4
3
3
2
2
2
2
2
Application
ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
170
101
67
45
37
27
16
12
8
8
6
4
3
2
1
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Available images

Catalog No. ABIN6656561
$ 394.90
Plus shipping costs $45.00 and $22.60 dry ice

General

Antigen GATA Binding Protein 3 (GATA3) Antibodies
Epitope C-Term
(43), (17), (15), (15), (12), (10), (9), (9), (9), (8), (8), (8), (7), (6), (6), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(250), (129), (54), (15), (13), (10), (8), (7), (7), (5), (5), (4), (4), (2), (1)
Host Rabbit
(153), (76), (21), (8)
Clonality
Polyclonal
Conjugate This GATA3 antibody is un-conjugated
(11), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2)
Application ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
(170), (101), (67), (45), (37), (27), (16), (12), (8), (8), (6), (4), (3), (2), (1)
Supplier

Product Details anti-GATA3 Antibody

Target Details GATA3 Application Details Handling Images
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) Cross reactivity with GATA3 from other sources has not been determined.
Purification Anti-GATA3 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Immunogen

Immunogen: Anti-GATA3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide near the C-terminus of human GATA3.

Immunogen Type: Peptide

Isotype IgG
Plasmids, Primers & others

Target Details GATA3

Product Details anti-GATA3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GATA3 (GATA3 Antibody Abstract)
Background

Synonyms: GATA3 Antibody, HDR, HDRS, Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3

Background: GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer.

Gene Name: GATA3

Gene ID 2625
NCBI Accession NP_001002295
UniProt P23771
Pathways Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation

Application Details

Product Details anti-GATA3 Antibody Target Details GATA3 Handling Images back to top
Application Notes

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-GATA3 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 48 kDa in Western Blots of specific cell lysates and tissues.

ELISA Dilution: 1:10,000-1:20,000

Western Blot Dilution: 1-2 μg/mL

IF Microscopy Dilution: 20 μg/mL

Restrictions For Research Use only

Handling

Product Details anti-GATA3 Antibody Target Details GATA3 Application Details Images back to top
Format Liquid
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Images

Product Details anti-GATA3 Antibody Target Details GATA3 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-GATA Binding Protein 3 (GATA3) (C-Term) antibody (ABIN6656561) Immunofluorescence of GATA3 Antibody Immunofluorescence Microscopy of GATA3 antibody....