Rat (Rattus) INHBA ELISA Kit

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Antigen
Inhibin, beta A (INHBA) ELISA Kits
  • actba
  • inhba
  • inhbaa-001
  • edf
  • frp
  • EDF
  • FRP
  • INHB
  • inhibin, beta Aa
  • inhibin, beta A
  • inhibin beta A
  • inhibin beta-A
  • inhbaa
  • INHBA
  • inhba
  • LOC100354786
  • Inhba
Epitope
AA 311-426
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
15
15
9
5
4
2
2
2
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Options
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Catalog No. ABIN411256
$ 320.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.541376 ABIN431589 Sandwich ELISA Serum, Plasma, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
10.541376 ABIN1570774 Sandwich ELISA Biological Fluids, Plasma, Serum 15.625-1000 pg/mL 15.625 pg/mL Log in to see
10.541376 ABIN2708665 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN841599 Sandwich ELISA Serum, Plasma, Tissue Homogenate 8-200 pg/mL 8 pg/mL Log in to see
1 ABIN578861 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN5655347 Sandwich ELISA Biological Fluids, Plasma, Serum 15.62 pg/mL - 1000 pg/mL 15.62 pg/mL Log in to see
1 ABIN5592814 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN771970 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
1 ABIN5655348 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 1.6 pg/mL - 400 pg/mL 1.6 pg/mL Log in to see
1 ABIN5521819 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2684746 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Serum, Tissue Lysate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN2534985 Sandwich ELISA Serum, Plasma, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
1 ABIN4970116 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2948477 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN5073878 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

General

Antigen Inhibin, beta A (INHBA) ELISA Kits
Epitope AA 311-426
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(15), (15), (9), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details INHBA ELISA Kit

Target details Application Details Handling References for INHBA Kit (ABIN411256) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Activin A
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: CHO
Immunogen sequence: G311-S426
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Predicted Reactivity Bovine,Monkey,Rabbit
Sensitivity <12pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: CHO
Immunogen sequence: G311-S426

Target details

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Antigen
Alternative Name INHBA (INHBA ELISA Kit Abstract)
Background

Protein Function: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Background: Activin A is a homodimer of 14 kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

Synonyms: Inhibin beta A chain,Activin beta-A chain,Inhba,

Full Gene Name: Inhibin beta A chain

Cellular Localisation: Secreted.
Gene ID 29200
UniProt P18331
Pathways Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Autophagy

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated
Protocol rat Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Activin A amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat Activin A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat Activin A standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 97, Standard deviation: 4.9, CV(%): 5
  • Sample 2: n=16, Mean(pg/ml): 294, Standard deviation: 14.7, CV(%): 5
  • Sample 3: n=16, Mean(pg/ml): 459, Standard deviation: 23.9, CV(%): 5.2,
  • Sample 1: n=24, Mean(pg/ml): 103, Standard deviation: 8.0, CV(%): 7.8
  • Sample 2: n=24, Mean(pg/ml): 315, Standard deviation: 19.8, CV(%): 6.3
  • Sample 3: n=24, Mean(pg/ml): 512, Standard deviation: 24.6, CV(%): 4.8
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for INHBA Kit (ABIN411256)

Product Details INHBA ELISA Kit Target details Application Details Handling Images back to top
Background publications

Di Loreto, Reis, Cataldi, Zuiani, Luisi, Beltrami, Petraglia: "Human mammary gland and breast carcinoma contain immunoreactive inhibin/activin subunits: evidence for a secretion into cystic fluid." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 141, Issue 2, pp. 190-4, 1999

Images

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Supplier Images
ELISA image for Inhibin, beta A (INHBA) ELISA Kit (ABIN411256) Rat Activin A PicoKine ELISA Kit standard curve