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Z-VAD-FMK Inhibitor

Func, CC, BR, InhA Cell-permeable 187389-52-2 Z-VAD-FMK, Z-VAD-fluoromethylketone, Caspase-Family Inhibitor (fluoromethylketone)
Pubmed (20)
Catalog No. ABIN412070
$225.00
Plus shipping costs $45.00
1 mg ABIN412070
1 mg ABIN412070
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Application
    Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
    Purpose
    A pan-caspase inhibitor
    Sequence
    Z-Val-Ala-Asp(OMe)-FMK
    Characteristics
    A synthetic peptide that irreversibly inhibits activity of caspase family proteases and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
    Purity
    Single spot by TLC
    Chemical Name
    Z-VAD-FMK, Z-VAD-fluoromethylketone, Caspase-Family Inhibitor (fluoromethylketone)
    Formula
    C₂₂H₃₀N₃O₇F
    Permeability
    Cell-permeable
    Solubility
    DMSO (20 mM)
  • Application Notes
    We recommend using 2-10 µM for inhibiting caspase activity in cell culture.
    The optimal doses may vary for different cells and culture conditions.
    Restrictions
    For Research Use only
  • Format
    Solid, Lyophilized
    Handling Advice
    Protect from light and moisture
    Storage
    -20 °C
    Expiry Date
    12 months
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    Wang, Wang, Zhou, Wei: "Farnesol induces apoptosis-like cell death in the pathogenic fungus Aspergillus flavus." in: Mycologia, Vol. 106, Issue 5, pp. 881-8, 2014 (PubMed).

    Hashimoto, Todo: "Mitotic slippage underlies the relationship between p53 dysfunction and the induction of large micronuclei by colcemid." in: Mutagenesis, Vol. 28, Issue 4, pp. 457-64, 2013 (PubMed).

    Saha, Jiang, Yang, Reece, Chang: "PRIMA-1Met/APR-246 displays high antitumor activity in multiple myeloma by induction of p73 and Noxa." in: Molecular cancer therapeutics, Vol. 12, Issue 11, pp. 2331-41, 2013 (PubMed).

    Breinig, Mayer, Harjung, Goeppert, Malz, Penzel, Neumann, Hartmann, Dienemann, Giaccone, Schirmacher, Kern, Chiosis, Rieker: "Heat shock protein 90-sheltered overexpression of insulin-like growth factor 1 receptor contributes to malignancy of thymic epithelial tumors." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 8, pp. 2237-49, 2011 (PubMed).

    Ariazi, Cunliffe, Lewis-Wambi, Slifker, Willis, Ramos, Tapia, Kim, Yerrum, Sharma, Nicolas, Balagurunathan, Ross, Jordan: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).

    Hara, Senga, Biswas, Hasegawa, Ito, Hyodo, Hirooka, Niwa, Goto, Hamaguchi: "Recovery of anoikis in Src-transformed cells and human breast carcinoma cells by restoration of the SIRP α1/SHP-2 signaling system." in: Cancer research, Vol. 71, Issue 4, pp. 1229-34, 2011 (PubMed).

    Xie, Jiang, Wan, Du, Guo, Liu, Ye, Niu, Wu, Dong, Zhang: "Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway." in: Journal of molecular cell biology, Vol. 3, Issue 4, pp. 250-9, 2011 (PubMed).

    Dolai, Pal, Yadav, Adak: "Endoplasmic reticulum stress-induced apoptosis in Leishmania through Ca2+-dependent and caspase-independent mechanism." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13638-46, 2011 (PubMed).

    Ariyama, Kono, Matsuda, Inoue, Arai: "Decrease in membrane phospholipid unsaturation induces unfolded protein response." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 22027-35, 2010 (PubMed).

    Fukazawa, Maeda, Matsuoka, Yamatsuji, Shigemitsu, Morita, Faiola, Durbin, Soucek, Naomoto: "Inhibition of Myc effectively targets KRAS mutation-positive lung cancer expressing high levels of Myc." in: Anticancer research, Vol. 30, Issue 10, pp. 4193-200, 2010 (PubMed).

    Gao, Bauvy, Souquère, Tonelli, Liu, Zhu, Qiao, Bakula, Proikas-Cezanne, Pierron, Codogno, Chen, Mehrpour: "The Bcl-2 homology domain 3 mimetic gossypol induces both Beclin 1-dependent and Beclin 1-independent cytoprotective autophagy in cancer cells." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25570-81, 2010 (PubMed).

    Saha, Jiang, Mukai, Chang: "RITA inhibits multiple myeloma cell growth through induction of p53-mediated caspase-dependent apoptosis and synergistically enhances nutlin-induced cytotoxic responses." in: Molecular cancer therapeutics, Vol. 9, Issue 11, pp. 3041-51, 2010 (PubMed).

    Ito, Yip, Katz, Fonseca, Hedley, Chow, Xu, Wood, Bastianutto, Schimmer, Kelley, Liu: "Potential use of cetrimonium bromide as an apoptosis-promoting anticancer agent for head and neck cancer." in: Molecular pharmacology, Vol. 76, Issue 5, pp. 969-83, 2009 (PubMed).

    Liu, Deng, Lancto, Abrahamsen, Rutherford, Enomoto: "Biphasic modulation of apoptotic pathways in Cryptosporidium parvum-infected human intestinal epithelial cells." in: Infection and immunity, Vol. 77, Issue 2, pp. 837-49, 2009 (PubMed).

    Dai, Liu, Tang, DeSano, Burstein, Davis, Pienta, Lawrence, Xu: "Molecularly targeted radiosensitization of human prostate cancer by modulating inhibitor of apoptosis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 23, pp. 7701-10, 2008 (PubMed).

    Liu, Enomoto, Lancto, Abrahamsen, Rutherford: "Inhibition of apoptosis in Cryptosporidium parvum-infected intestinal epithelial cells is dependent on survivin." in: Infection and immunity, Vol. 76, Issue 8, pp. 3784-92, 2008 (PubMed).

    Upton, Austgen, Nishino, Coakley, Hagen, Han, Papa, Oakes: "Caspase-2 cleavage of BID is a critical apoptotic signal downstream of endoplasmic reticulum stress." in: Molecular and cellular biology, Vol. 28, Issue 12, pp. 3943-51, 2008 (PubMed).

    Niikura, Dixit, Scott, Perkins, Kitagawa: "BUB1 mediation of caspase-independent mitotic death determines cell fate." in: The Journal of cell biology, Vol. 178, Issue 2, pp. 283-96, 2007 (PubMed).

  • Molecular Weight
    467.5 g/mol
    CAS-No
    187389-52-2
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