anti-Mouse (Murine) ITGA5 antibody for Immunohistochemistry (Frozen Sections)

Recommended ITGA5 Antibody

Antigen
Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) Antibodies
  • ITGA5
  • Cd49e
  • Fnra
  • VLA5
  • CD49e
  • FNRA
  • VLA5A
  • Itga5_retired
  • integrin subunit alpha 5
  • integrin alpha 5 (fibronectin receptor alpha)
  • ITGA5
  • Itga5
Reactivity
Mouse (Murine)
197
150
56
5
4
2
2
1
1
1
Host
Rat
148
57
53
10
2
1
1
Clonality
Monoclonal
Conjugate
This ITGA5 antibody is un-conjugated
28
22
13
6
4
4
4
4
3
3
3
3
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
137
134
88
78
31
29
18
14
13
7
7
5
3
2
2
1
1
Options
Catalog No. ABIN2689316
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
10 ABIN1176992 BR IHC (f) IHC (fro) FACS IF Rat IgG2a, kappa 5H10-27 8
8.5 ABIN2480796 IHC (fro) FACS Rat IgG2a MFR5 (5H10) 4
4 ABIN120000 FACS IHC (fro) Rat IgG2a MFR5 (5H10) 2
1 ABIN370976 Func FACS IHC (fro) Rat IgG2a 5H10-27 6
1 ABIN370977 Func FACS IHC (fro) Rat IgG2a 5H10-27 6
1 ABIN226403 FACS IHC IHC (fro) Biotin Rat IgG2a, kappa Subunit 5 alpha MFR5 0
1 ABIN198796 FACS IHC IHC (fro) PE Rat IgG2a, kappa Subunit 5 alpha MFR5 0
1 ABIN198794 FACS IHC IHC (fro) FITC Rat IgG2a, kappa Subunit 5 alpha MFR5 0
1 ABIN226402 FACS IHC IHC (fro) Rat IgG2a, kappa Subunit 5 alpha MFR5 0
1 ABIN226398 FACS IHC IHC (fro) Rat IgG2a, kappa Subunit 5 alpha MFR5 0
1 ABIN2610607 FACS IHC IHC (fro) Rat IgG2a 5H10-27 0
1 ABIN2610604 FACS IHC IHC (fro) Biotin Rat IgG2a 5H10-27 0
1 ABIN2610603 FACS IHC IHC (fro) FITC Rat IgG2a 5H10-27 0
1 ABIN206496 FACS IHC IHC (fro) Rat IgG2a MFR5 (5H10) 0
1 ABIN206728 FACS IHC IHC (fro) FITC Rat IgG2a 0
1 ABIN2610605 FACS IHC IHC (fro) Rat IgG2a 5H10-27 0
1 ABIN642724 IHC (fro) FACS Rat 0

General

Antigen Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) Antibodies
Reactivity Mouse (Murine)
(197), (150), (56), (5), (4), (2), (2), (1), (1), (1)
Host Rat
(148), (57), (53), (10), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ITGA5 antibody is un-conjugated
(28), (22), (13), (6), (4), (4), (4), (4), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(137), (134), (88), (78), (31), (29), (18), (14), (13), (7), (7), (5), (3), (2), (2), (1), (1)
Pubmed 10 references available
Supplier

Product Details anti-ITGA5 Antibody

Target Details ITGA5 Application Details Handling References for anti-ITGA5 antibody (ABIN2689316) Images
Brand BD Pharmingen™
Characteristics The 5H10-27 (MFR5) antibody reacts with the α5 chain of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, mast cells, and a variety of mouse cell lines, but not splenocytes. Soluble 5H10-27 (MFR5) antibody has been reported to inhibit VLA-5-mediated functions in vitro. In addition, immobilized mAb 5H10-27 (MFR5) has been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3e antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD49e - Purified - Clone 5H10-27 (MFR5) - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse mast cell line MC/9
Clone 5H10
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details ITGA5

Product Details anti-ITGA5 Antibody Application Details Handling References for anti-ITGA5 antibody (ABIN2689316) Images back to top
Antigen
Alternative Name CD49e (ITGA5 Antibody Abstract)
Background Synonyms: Integrin α5 chain
Pathways Integrin Complex

Application Details

Product Details anti-ITGA5 Antibody Target Details ITGA5 Handling References for anti-ITGA5 antibody (ABIN2689316) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-ITGA5 Antibody Target Details ITGA5 Application Details References for anti-ITGA5 antibody (ABIN2689316) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-ITGA5 antibody (ABIN2689316)

Product Details anti-ITGA5 Antibody Target Details ITGA5 Application Details Handling Images back to top
Product cited in:

Makwana, Werner, Acosta-Saltos, Gonitel, Pararajasingam, Pararajasingham, Ruff, Rumajogee, Cuthill, Galiano, Bohatschek, Wallace, Anderson, Mayer, Behrens, Raivich: "Peripheral facial nerve axotomy in mice causes sprouting of motor axons into perineuronal central white matter: time course and molecular characterization." in: The Journal of comparative neurology, Vol. 518, Issue 5, pp. 699-721, 2010

Cukierman, Pankov, Stevens, Yamada: "Taking cell-matrix adhesions to the third dimension." in: Science (New York, N.Y.), Vol. 294, Issue 5547, pp. 1708-12, 2001

Yang, Hynes: "Fibronectin receptor functions in embryonic cells deficient in alpha 5 beta 1 integrin can be replaced by alpha V integrins." in: Molecular biology of the cell, Vol. 7, Issue 11, pp. 1737-48, 1997

Rich, Van Nood, Lee: "Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2916-23, 1996

Ruppert, Aigner, Hubbe, Yagita, Altevogt: "The L1 adhesion molecule is a cellular ligand for VLA-5." in: The Journal of cell biology, Vol. 131, Issue 6 Pt 2, pp. 1881-91, 1996

Uhlenkott, Huijzer, Cardeiro, Elstad, Meadows: "Attachment, invasion, chemotaxis, and proteinase expression of B16-BL6 melanoma cells exhibiting a low metastatic phenotype after exposure to dietary restriction of tyrosine and phenylalanine." in: Clinical & experimental metastasis, Vol. 14, Issue 2, pp. 125-37, 1996

Gomez, Cano: "Expression of beta 1 integrin receptors in transformed mouse epidermal keratinocytes: upregulation of alpha 5 beta 1 in spindle carcinoma cells." in: Molecular carcinogenesis, Vol. 12, Issue 3, pp. 153-65, 1995

Halvorson, Coligan: "Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4567-74, 1995

Hardy, Minguell: "Modulation of the adhesion of hemopoietic progenitor cells to the RGD site of fibronectin by interleukin 3." in: Journal of cellular physiology, Vol. 164, Issue 2, pp. 315-23, 1995

Kinashi, Springer: "Adhesion molecules in hematopoietic cells." in: Blood cells, Vol. 20, Issue 1, pp. 25-44, 1995