anti-Human PKC delta antibody for Immunocytochemistry

Recommended PKC delta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, delta (PKCd) Antibodies
  • MAY1
  • PKCD
  • nPKC-delta
  • AI385711
  • D14Ertd420e
  • PKC[d]
  • PKCdelta
  • Pkcd
  • fb09d10
  • fv43b11
  • prkcd
  • wu:fb09d10
  • wu:fv43b11
  • zgc:56558
  • PKC-delta
  • may1
  • npkc-delta
  • pkc-delta2
  • pkcd
  • tpa-1
  • protein kinase C delta
  • protein kinase C, delta
  • protein kinase C, delta a
  • protein kinase C delta L homeolog
  • PRKCD
  • Prkcd
  • prkcda
  • prkcd.L
  • prkcd
Epitope
pSer664
37
27
25
23
15
14
12
11
10
10
9
7
6
6
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
295
180
156
15
6
4
4
4
3
3
2
1
Host
Rabbit
299
25
Clonality
Polyclonal
Conjugate
This PKC delta antibody is un-conjugated
10
10
7
5
5
4
3
3
3
3
3
3
3
3
3
2
2
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
275
144
133
47
39
31
18
13
13
5
3
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN6271530
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13 ABIN6255881 ELISA ICC IF IHC WB Rabbit IgG pTyr313 Log in to see Polyclonal 0
13 ABIN6264244 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN6256394 ELISA ICC IF IHC WB Rabbit IgG pSer645 Log in to see Polyclonal 0
10 ABIN6256779 ELISA ICC IF WB Rabbit IgG pTyr64 Log in to see Polyclonal 0
10 ABIN6264245 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN6264253 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN1078462 ICC IHC WB Rabbit IgG AA 330-672 Log in to see Polyclonal 0
1 ABIN3201398 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see 0

General

Antigen Protein Kinase C, delta (PKCd) Antibodies
Epitope pSer664
(37), (27), (25), (23), (15), (14), (12), (11), (10), (10), (9), (7), (6), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(295), (180), (156), (15), (6), (4), (4), (4), (3), (3), (2), (1)
Host Rabbit
(299), (25)
Clonality
Polyclonal
Conjugate This PKC delta antibody is un-conjugated
(10), (10), (7), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(275), (144), (133), (47), (39), (31), (18), (13), (13), (5), (3), (2), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-PKC delta Antibody

Target Details PKC delta Application Details Handling Images
Specificity PKCD (Phospho-Ser664) Antibody detects endogenous levels of PKCD only when phosphorylated at Ser664
Cross-Reactivity Human, Mouse (Murine)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human PKCD (Phospho-Ser664)
Isotype IgG
Plasmids, Primers & others

Target Details PKC delta

Product Details anti-PKC delta Antibody Application Details Handling Images back to top
Antigen
Alternative Name PKCD (PKCd Antibody Abstract)
Background

Description: Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Negatively regulates B cell proliferation and also has an important function in self-antigen induced B cell tolerance induction. Upon DNA damage, activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Is highly expressed in a number of cancer cells and promotes cell survival and resistance against chemotherapeutic drugs by inducing cyclin D1 (CCND1) and hyperphosphorylation of RB1, and via several pro-survival pathways, including NF-kappa-B, AKT1 and MAPK1/3 (ERK1/2). Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl-leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion, acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. The catalytic subunit phosphorylates 14-3-3 proteins (YWHAB, YWHAZ and YWHAH) in a sphingosine-dependent fashion (By similarity). Phosphorylates ELAVL1 in response to angiotensin-2 treatment (PubMed:18285462).

Gene: PRKCD

Molecular Weight 78kDa
Gene ID 5580
UniProt Q05655
Pathways Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism

Application Details

Product Details anti-PKC delta Antibody Target Details PKC delta Handling Images back to top
Application Notes WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-PKC delta Antibody Target Details PKC delta Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-PKC delta Antibody Target Details PKC delta Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Protein Kinase C, delta (PKCd) (pSer664) antibody (ABIN6271530) ABIN6271531 at 1/200 staining Human kidney cancer tissue sections by IHC-P. The tissu...
Immunohistochemistry (IHC) image for anti-Protein Kinase C, delta (PKCd) (pSer664) antibody (ABIN6271530) ABIN6271531 at 1/200 staining Mouse intestinal tissue sections by IHC-P. The tissue w...
Immunohistochemistry (IHC) image for anti-Protein Kinase C, delta (PKCd) (pSer664) antibody (ABIN6271530) ABIN6271531 at 1/200 staining Rat testis tissue sections by IHC-P. The tissue was for...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Protein Kinase C, delta (PKCd) (pSer664) antibody (ABIN6271530) ABIN6271531 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabili...
Western Blotting (WB) image for anti-Protein Kinase C, delta (PKCd) (pSer664) antibody (ABIN6271530) Western blot analysis of PKCD (Phospho-Ser664) using UV treated 293 whole cell lysates