anti-Mouse (Murine) PKC delta antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PKC delta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, delta (PKCd) Antibodies
  • MAY1
  • PKCD
  • nPKC-delta
  • AI385711
  • D14Ertd420e
  • PKC[d]
  • PKCdelta
  • Pkcd
  • fb09d10
  • fv43b11
  • prkcd
  • wu:fb09d10
  • wu:fv43b11
  • zgc:56558
  • PKC-delta
  • may1
  • npkc-delta
  • pkc-delta2
  • pkcd
  • tpa-1
  • protein kinase C delta
  • protein kinase C, delta
  • protein kinase C, delta a
  • protein kinase C delta L homeolog
  • PRKCD
  • Prkcd
  • prkcda
  • prkcd.L
  • prkcd
Epitope
AA 643-647
37
27
25
23
15
14
12
11
10
10
9
7
6
6
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
295
180
155
15
6
4
4
4
3
3
2
1
Host
Rabbit
299
25
Clonality
Polyclonal
Conjugate
This PKC delta antibody is un-conjugated
10
10
7
5
5
4
3
3
3
3
3
3
3
3
3
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
275
145
134
46
39
31
18
14
13
5
3
2
1
1
1
1
Supplier
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Catalog No. ABIN197564
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN197068 IHC (p) WB Rabbit pSer645 Log in to see Polyclonal 2
7 ABIN272063 IHC (p) WB Rabbit Log in to see Polyclonal 0
4 ABIN2879020 ELISA IHC IHC (p) WB Rabbit IgG AA 471-520 Log in to see Polyclonal 0
4 ABIN703270 IF (p) IHC (p) WB Rabbit IgG AA 280-330, pTyr313 Log in to see Polyclonal 0
4 ABIN5957386 ELISA IHC (p) WB Rabbit IgG Thr507 Log in to see Polyclonal 0
4 ABIN5957389 ELISA IHC (p) WB Rabbit IgG pThr507 Log in to see Polyclonal 0
4 ABIN701785 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN1738340 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN703279 IHC (p) WB HRP Rabbit IgG AA 280-330, pTyr313 Log in to see Polyclonal 0
1 ABIN703272 IHC (p) WB Biotin Rabbit IgG AA 280-330, pTyr313 Log in to see Polyclonal 0
1 ABIN703249 IHC (p) WB HRP Rabbit IgG pTyr52 Log in to see Polyclonal 0
1 ABIN2884230 ELISA IHC IHC (p) WB Rabbit IgG pTyr313 Log in to see Polyclonal 0
1 ABIN2884231 ELISA IF IHC IHC (p) WB Rabbit IgG pTyr52 Log in to see Polyclonal 0
1 ABIN2891817 IHC IHC (p) WB Rabbit Gly499 Log in to see Polyclonal 0
1 ABIN2886136 ELISA IHC IHC (p) WB Rabbit IgG AA 30-79 Log in to see Polyclonal 0
1 ABIN701794 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN703242 IHC (p) WB Biotin Rabbit IgG pTyr52 Log in to see Polyclonal 0
1 ABIN703240 IF (p) IHC (p) WB Rabbit IgG pTyr52 Log in to see Polyclonal 0
1 ABIN2576945 IHC IHC (p) WB Rabbit pThr507 Log in to see Polyclonal 0
1 ABIN701787 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal 0

General

Antigen Protein Kinase C, delta (PKCd) Antibodies
Epitope AA 643-647
(37), (27), (25), (23), (15), (14), (12), (11), (10), (10), (9), (7), (6), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(295), (180), (155), (15), (6), (4), (4), (4), (3), (3), (2), (1)
Host Rabbit
(299), (25)
Clonality
Polyclonal
Conjugate This PKC delta antibody is un-conjugated
(10), (10), (7), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(275), (145), (134), (46), (39), (31), (18), (14), (13), (5), (3), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-PKC delta Antibody

Target Details PKC delta Application Details Handling References for anti-PKC delta antibody (ABIN197564) Images
Specificity This antibody detects endogenous levels of total PKC-delta protein.
Purification Immunoaffinity Chromatography using epitope-specific immunogen.
Immunogen Peptide sequence around amino acids 643~647 (R-L-Sp-Y-S) derived from Human PKC- delta
Plasmids, Primers & others

Target Details PKC delta

Product Details anti-PKC delta Antibody Application Details Handling References for anti-PKC delta antibody (ABIN197564) Images back to top
Antigen
Alternative Name PRKCD (PKCd Antibody Abstract)
Background Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.Synonyms: PKC delta, PKC-delta, PKCD, Protein kinase C delta type
Gene ID 5580
NCBI Accession NP_006245
UniProt Q05655
Pathways Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism

Application Details

Product Details anti-PKC delta Antibody Target Details PKC delta Handling References for anti-PKC delta antibody (ABIN197564) Images back to top
Application Notes Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PKC delta Antibody Target Details PKC delta Application Details References for anti-PKC delta antibody (ABIN197564) Images back to top
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.

References for anti-PKC delta antibody (ABIN197564)

Product Details anti-PKC delta Antibody Target Details PKC delta Application Details Handling Images back to top
Background publications

Van Kolen, Slegers: "Atypical PKCzeta is involved in RhoA-dependent mitogenic signaling by the P2Y(12) receptor in C6 cells." in: The FEBS journal, Vol. 273, Issue 8, pp. 1843-54, 2006

Zhang, Keane, Zhu, Sharma, Rozengurt, Strieter, Dubinett, Huang: "Interleukin-7 and transforming growth factor-beta play counter-regulatory roles in protein kinase C-delta-dependent control of fibroblast collagen synthesis in pulmonary fibrosis." in: The Journal of biological chemistry, Vol. 279, Issue 27, pp. 28315-9, 2004

Sakamoto, Aschenbach, Hirshman, Goodyear: "Akt signaling in skeletal muscle: regulation by exercise and passive stretch." in: American journal of physiology. Endocrinology and metabolism, Vol. 285, Issue 5, pp. E1081-8, 2003

Villalba, Bi, Hu, Altman, Bushway, Reits, Neefjes, Baier, Abraham, Altman: "Translocation of PKC[theta] in T cells is mediated by a nonconventional, PI3-K- and Vav-dependent pathway, but does not absolutely require phospholipase C." in: The Journal of cell biology, Vol. 157, Issue 2, pp. 253-63, 2002

Images

Product Details anti-PKC delta Antibody Target Details PKC delta Application Details Handling References for anti-PKC delta antibody (ABIN197564) back to top
Supplier Images
 image for anti-Protein Kinase C, delta (PKCd) (AA 643-647) antibody (ABIN197564) anti-Protein Kinase C, delta (PKCd) (AA 643-647) antibody
 image for anti-Protein Kinase C, delta (PKCd) (AA 643-647) antibody (ABIN197564) anti-Protein Kinase C, delta (PKCd) (AA 643-647) antibody (Image 2)
 image for anti-Protein Kinase C, delta (PKCd) (AA 643-647) antibody (ABIN197564) anti-Protein Kinase C, delta (PKCd) (AA 643-647) antibody (Image 3)