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HEK-293 Cells IgG isotype control (AVI tag,Biotin)

IsoC IgG AVI tag,Biotin Fc fragment
Catalog No. ABIN2870572
  • Target See all IgG products
    IgG
    Fragment
    Fc fragment
    Biological Activity
    Active
    Reactivity
    Mouse
    Host
    • 142
    • 140
    • 124
    • 89
    • 89
    • 57
    • 56
    • 47
    • 46
    • 44
    • 38
    • 37
    • 34
    • 33
    • 27
    • 21
    • 16
    • 15
    • 9
    • 9
    • 4
    • 1
    HEK-293 Cells
    Conjugate
    AVI tag,Biotin
    Application
    Isotype Control (IsoC)
    Brand
    MABSol®,PrecisionAvi
    Specificity
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Characteristics
    This protein carries an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 28.2 kDa. The protein migrates as 36 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
    Purity
    >95 % as determined by SDS-PAGE.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Isotype
    IgG
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
    Buffer
    50 mM Tris, 100 mM Glycine, pH 7.5
    Storage
    -20 °C
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
    Molecular Weight
    28.2 kDa
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