Rat IgG2c Isotype Control
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- Target See all IgG2c products
- IgG2c
- Reactivity
- Rat
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Host
- Rat
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Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS), ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Purification
- RAT IgG2c Kappa (κ) isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1a, IgG1b, IgG3 , IgM , and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
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Clone
- RG2cK
- Isotype
- IgG2c
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Rat IgG2c isotype control
FACS, ELISA, FLISA Monoclonal SB68b IgG2c unconjugated
Rat IgG2c isotype control (PE)FACS, ELISA, FLISA Monoclonal SB68b IgG2c PE
Rat IgG2c isotype controlFACS, ELISA, FLISA Monoclonal SB68b IgG2c unconjugated
Rat IgG2c isotype control (APC)FACS, ELISA, FLISA Monoclonal SB68b IgG2c APC
Mouse IgG2c isotype control (Biotin)FACS, ELISA Monoclonal 6-3 IgG2c Biotin
Mouse IgG2c isotype controlIsoC, ELISA, IHC Monoclonal 6-3 IgG2c unconjugated
Rat IgG2c isotype control (Biotin)FACS, ELISA, FLISA Monoclonal SB68b IgG2c Biotin
Rat IgG2c isotype control (FITC)FACS, ELISA, FLISA Monoclonal SB68b IgG2c FITC
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- Application Notes
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Flow Cytometry Dilution: 1:1000 - 1:5000
Application Note: RAT IgG2c Kappa (κ) isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
- Comment
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RAT IgG2c Kappa (κ) isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain α - IgA, γ - IgG 1, 2a, 2b, 2c and μ - IgM, light chain κ and λ.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target
- IgG2c
- Abstract
- IgG2c Products
- Target Type
- Antibody
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