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Llama IgG isotype control (HRP)

ELISA, FACS, WB IgG HRP
Catalog No. ABIN5633241
  • Target See all IgG products
    IgG
    Host
    • 140
    • 136
    • 119
    • 89
    • 87
    • 57
    • 56
    • 47
    • 46
    • 44
    • 38
    • 37
    • 34
    • 33
    • 27
    • 21
    • 16
    • 14
    • 9
    • 9
    • 4
    • 2
    Llama
    Conjugate
    HRP
    Application
    ELISA, Flow Cytometry (FACS), Western Blotting (WB)
    Purification
    Llama IgG has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain), non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa, non-reduced llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa.
    Isotype
    IgG
  • Application Notes

    Flow Cytometry Dilution: User Optimized

    Application Note: Llama IgG Peroxidase can be utilized as a control or standard reagent in SDS, Western Blotting, Flow Cytometry, and ELISA experiments. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do NOT add Sodium Azide!
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
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