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|Antigen||Angiotensin II Receptor, Type 1 (AGTR1) Antibodies|
|Epitope||AA 4-18, Extracellular Loop, N-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Angiotensin II Type-1 Receptor antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-Angiotensin II Type-1 Receptor AntibodyTarget Details Angiotensin II Type-1 Receptor Application Details Handling Images
|Cross-Reactivity (Details)||Mouse, rat, rabbit, dog - identical.|
Confirmed by amino acid analysis.
Antibody concentration after reconstitution:
1 µg peptide per 1 µg antibody.
|Purification||Affinity purified on immobilized antigen.|
|Immunogen||Peptide corresponding to amino acid residues 4-18 of human AT1 receptor. Extracellular, N-terminus.|
Target Details Angiotensin II Type-1 ReceptorProduct Details anti-Angiotensin II Type-1 Receptor Antibody Application Details Handling Images back to top
|Alternative Name||Angiotensin II Receptor Type-1 (AGTR1 Antibody Abstract)|
Angiotensin II receptor type 1 or AT1 receptor is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 receptor a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by InsP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration. In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc. As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.
|Pathways||JAK-STAT Signaling, ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour|
Application DetailsProduct Details anti-Angiotensin II Type-1 Receptor Antibody Target Details Angiotensin II Type-1 Receptor Handling Images back to top
WB: approx. 1:200,
ICC: approx. 1:100
|Restrictions||For Research Use only|
HandlingProduct Details anti-Angiotensin II Type-1 Receptor Antibody Target Details Angiotensin II Type-1 Receptor Application Details Images back to top
|Reconstitution||0.2 mL deionized water.|
|Buffer||Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % NaN3.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
|Storage||4 °C/-20 °C|
|Storage Comment||The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, small aliquots should be stored at -20 °C or below. The further dilutions should be made using a carrier protein such as BSA (1 %).|
ImagesProduct Details anti-Angiotensin II Type-1 Receptor Antibody Target Details Angiotensin II Type-1 Receptor Application Details Handling back to top