anti-Rat (Rattus) Angiotensin II Type-1 Receptor antibody for Western Blotting

Recommended Angiotensin II Type-1 Receptor Antibody (supplied by: Log in to see )

Antigen
Angiotensin II Receptor, Type 1 (AGTR1) Antibodies
  • AG2S
  • AGTR1A
  • AGTR1B
  • AT1
  • AT1AR
  • AT1B
  • AT1BR
  • AT1R
  • AT2R1
  • AT2R1A
  • AT2R1B
  • HAT1R
  • 1810074K20Rik
  • AI551199
  • AT1a
  • Agtr-1a
  • Agtr1
  • Angtr-1a
  • XAT-1
  • agtr1-A
  • agtr1.2
  • AT1-R
  • at1
  • AGTR1
  • agtr1
  • agtr1-a
  • agtr1-b
  • xAT
  • angiotensin II receptor type 1
  • angiotensin II receptor, type 1a
  • angiotensin II receptor type 1 S homeolog
  • uncharacterized AGTR1
  • angiotensin II receptor type 1 L homeolog
  • AGTR1
  • Agtr1a
  • agtr1.S
  • Agtr1
  • agtr1.L
Epitope
AA 4-18, Extracellular Loop, N-Term
23
16
15
11
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
115
83
76
5
5
3
3
2
2
2
2
2
1
1
Host
Rabbit
100
17
5
4
4
Clonality
Polyclonal
Conjugate
This Angiotensin II Type-1 Receptor antibody is un-conjugated
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
105
61
47
33
20
16
13
7
5
4
3
1
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Catalog No. ABIN1742173
$ 832.43
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16.562979 ABIN634511 WB Rabbit N-Term Log in to see Polyclonal 0
15.302324 ABIN6256893 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 1
12.760654 ABIN6390660 ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.760654 ABIN289376 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.760654 ABIN289368 IF IHC ELISA WB Chicken IgG Log in to see Polyclonal 0
10.802324 ABIN615708 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
10 ABIN604701 ELISA IHC IHC (p) WB Rabbit IgG AA 201-250 Log in to see Polyclonal 0
7.8023243 ABIN2775286 WB Rabbit N-Term Log in to see Polyclonal 1
7.8023243 ABIN1535649 IF ELISA WB Rabbit IgG AA 101-150 Log in to see Polyclonal 0
7.8023243 ABIN1866589 ICC IHC WB Rabbit IgG AA 263-359 Log in to see Polyclonal 0
7.8023243 ABIN1030844 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal 0
7.8023243 ABIN6000012 IF/ICC IHC IP WB Rabbit AA 263-359 Log in to see Polyclonal 0
7.8023243 ABIN2427725 ELISA WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN1731610 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
4.8023243 ABIN1870872 WB Rabbit IgG Log in to see Polyclonal 0
4.8023243 ABIN2465366 ELISA WB Goat Internal Region Log in to see Polyclonal 0
4.8023243 ABIN2458631 ELISA WB Rabbit Log in to see Polyclonal 0
4.8023243 ABIN2974131 WB Rabbit IgG Log in to see Polyclonal 0
4.8023243 ABIN5856025 ELISA WB Goat Log in to see Polyclonal 0
4.8023243 ABIN2560976 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0

General

Antigen Angiotensin II Receptor, Type 1 (AGTR1) Antibodies
Epitope AA 4-18, Extracellular Loop, N-Term
(23), (16), (15), (11), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(115), (83), (76), (5), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(100), (17), (5), (4), (4)
Clonality
Polyclonal
Conjugate This Angiotensin II Type-1 Receptor antibody is un-conjugated
(4), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(105), (61), (47), (33), (20), (16), (13), (7), (5), (4), (3), (1)
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Product Details anti-Angiotensin II Type-1 Receptor Antibody

Target Details Angiotensin II Type-1 Receptor Application Details Handling Images
Sequence NSSTEDGIKR IQDDC
Cross-Reactivity (Details) Mouse, rat, rabbit, dog - identical.
Characteristics Peptide Confirmation:
Confirmed by amino acid analysis.

Antibody concentration after reconstitution:
0.7 mg/mL

Preadsorption Control:
1 µg peptide per 1 µg antibody.

GPCR
Purification Affinity purified on immobilized antigen.
Immunogen Peptide corresponding to amino acid residues 4-18 of human AT1 receptor. Extracellular, N-terminus.
Plasmids, Primers & others

Target Details Angiotensin II Type-1 Receptor

Product Details anti-Angiotensin II Type-1 Receptor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Angiotensin II Receptor Type-1 (AGTR1 Antibody Abstract)
Background Synonyms:
AGTR1

Angiotensin II receptor type 1 or AT1 receptor is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 receptor a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by InsP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration. In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc. As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.
Gene ID 185
UniProt P30556
Pathways JAK-STAT Signaling, ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour

Application Details

Product Details anti-Angiotensin II Type-1 Receptor Antibody Target Details Angiotensin II Type-1 Receptor Handling Images back to top
Application Notes Recommended Dilution:
WB: approx. 1:200,
ICC: approx. 1:100
Comment

Published Applications:
Western blot analysis (WB): - Rat heart lysate (1:250) (see Wang, H. et al. (2013)).
- Mouse lung lysate (1:1000) (see Rodriguez-Machado, M.G. et al. (2013)).
- Sheep brain medulla lysate (1:200) (see Marshall, A.C. et al. (2013)).
Indirect flow cytometry (IFC): - Mouse gastrocnemius muscle cells (1:100) (see Yoshida, T. et al. (2013)).
Immunocytochemistry (IC): - Mouse isolated myofibers (1:100) (see Yoshida, T. et al. (2013)).

Restrictions For Research Use only

Handling

Product Details anti-Angiotensin II Type-1 Receptor Antibody Target Details Angiotensin II Type-1 Receptor Application Details Images back to top
Format Lyophilized
Reconstitution 0.2 mL deionized water.
Buffer Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % NaN3.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
Storage 4 °C/-20 °C
Storage Comment The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, small aliquots should be stored at -20 °C or below. The further dilutions should be made using a carrier protein such as BSA (1 %).

Images

Product Details anti-Angiotensin II Type-1 Receptor Antibody Target Details Angiotensin II Type-1 Receptor Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Angiotensin II Receptor, Type 1 (AGTR1) (AA 4-18), (Extracellular Loop), (N-Term) antibody (ABIN1742173) Western blot analysis of mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4)...
Western Blotting (WB) image for anti-Angiotensin II Receptor, Type 1 (AGTR1) (AA 4-18), (Extracellular Loop), (N-Term) antibody (ABIN1742173) Western blot analysis of rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) mem...
Immunohistochemistry (IHC) image for anti-Angiotensin II Receptor, Type 1 (AGTR1) (AA 4-18), (Extracellular Loop), (N-Term) antibody (ABIN1742173) Expression of Angiotensin II Receptor Type-1 in mouse cerebellum Immunohistochemical ...
Immunocytochemistry (ICC) image for anti-Angiotensin II Receptor, Type 1 (AGTR1) (AA 4-18), (Extracellular Loop), (N-Term) antibody (ABIN1742173) xpression of Angiotensin II Receptor Type-1 in rat C6 glioma cells Immunocytochemical...
Flow Cytometry (FACS) image for anti-Angiotensin II Receptor, Type 1 (AGTR1) (AA 4-18), (Extracellular Loop), (N-Term) antibody (ABIN1742173) Indirect flow cytometry analysis of live intact human Jurkat T-cell leukemia cells: B...