anti-Mouse (Murine) CD25 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Reactivity
Mouse (Murine)
629
439
142
22
16
8
6
6
6
5
4
4
1
1
1
1
Host
Rat
593
372
165
12
6
4
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to Biotin
129
119
92
82
26
25
25
21
20
14
13
12
11
10
10
10
10
10
9
9
9
9
9
6
6
6
6
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
933
196
164
142
117
110
75
73
40
20
20
18
17
17
16
13
8
8
5
5
4
2
2
1
1
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN2689121
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN670371 IF (p) IHC (p) WB Rabbit IgG AA 258-268 Log in to see Polyclonal 0
4 ABIN1859413 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-235 Log in to see Polyclonal 0
2.3645334 ABIN153451 ICC IHC (fro) IF IHC (p) IHC Mouse IgG1 kappa Log in to see IL2R-1 5
1 ABIN670380 IHC (p) WB HRP Rabbit IgG AA 258-268 Log in to see Polyclonal 0
1 ABIN670373 IHC (p) WB Biotin Rabbit IgG AA 258-268 Log in to see Polyclonal 0
-0.6354666 ABIN4995166 CyTOF FACS IHC IHC (p) Rat IgG1 Log in to see AP-MAB0710 1
-5.1354666 ABIN153558 CyTOF FACS IHC IHC (fro) IHC (p) IP Rat IgG1 Log in to see PC61-5-3 0
-12.848514 ABIN4290998 ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see IL2R-1 0
-12.848514 ABIN4995172 FACS IHC IHC (p) DyLight 680 Rat IgG1 Log in to see AP-MAB0710 0
-12.848514 ABIN4995170 FACS IHC IHC (p) Alexa Fluor 700 Rat IgG1 Log in to see AP-MAB0710 0
-12.848514 ABIN4995168 FACS IHC IHC (p) Alexa Fluor 488 Rat IgG1 Log in to see AP-MAB0710 0
-12.848514 ABIN4995169 FACS IHC IHC (p) Alexa Fluor 647 Rat IgG1 Log in to see AP-MAB0710 0
-12.848514 ABIN4995173 FACS IHC IHC (p) DyLight 488 Rat IgG1 Log in to see AP-MAB0710 0
-12.848514 ABIN4995174 FACS IHC IHC (p) DyLight 755 Rat IgG1 Log in to see AP-MAB0710 0
-12.848514 ABIN4995167 FACS IHC IHC (p) Alexa Fluor 405 Rat IgG1 Log in to see AP-MAB0710 0
-12.848514 ABIN4995175 FACS IHC IHC (p) PerCP Rat IgG1 Log in to see AP-MAB0710 0
-12.848514 ABIN4995177 FACS IHC IHC (p) DyLight 350 Rat IgG1 Log in to see AP-MAB0710 0
-12.848514 ABIN4995178 FACS IHC IHC (p) DyLight 405 Rat IgG1 Log in to see AP-MAB0710 0
-12.848514 ABIN5945609 FACS IHC IHC (p) Janelia Fluor® 549 Rat IgG1 Log in to see AP-MAB0710 0
-12.848514 ABIN5945610 FACS IHC IHC (p) Janelia Fluor® 646 Rat IgG1 Log in to see AP-MAB0710 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(629), (439), (142), (22), (16), (8), (6), (6), (6), (5), (4), (4), (1), (1), (1), (1)
Host Rat
(593), (372), (165), (12), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to Biotin
(129), (119), (92), (82), (26), (25), (25), (21), (20), (14), (13), (12), (11), (10), (10), (10), (10), (10), (9), (9), (9), (9), (9), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(933), (196), (164), (142), (117), (110), (75), (73), (40), (20), (20), (18), (17), (17), (16), (13), (8), (8), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
Supplier Log in to see

Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN2689121) Images
Brand BD Pharmingen™
Characteristics The 7D4 antibody reacts with CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γ (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. no. 553068). The 3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by mAbs 7D4 and PC61 (Cat. no. 553866), and it blocks binding of IL-2 to CD25. Immunohistochemical staining of CD25 positive cells. Frozen sections of mouse thymus were reacted with the anti-CD25 antibody. Immature thymocytes expressing the IL-2 receptor can be identified by the brown labeling of their cell membranes. Amplification 20x.

BD Pharmingen™ Biotin Rat Anti-Mouse CD25 - Biotin - Clone 7D4 - Isotype Rat IgM, κ - Reactivity Ms - 1 mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Clone 7D4
Isotype IgM, kappa
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN2689121) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background Synonyms: IL-2 Receptor α chain, p55
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN2689121) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN2689121) Images back to top
Format Liquid
Concentration 125 μg/mL
Buffer Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD25 antibody (ABIN2689121)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Chen, Ma, Young, Alt: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, 1995

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Garni-Wagner, Witte, Tutt, Kuziel, Tucker, Bennett, Kumar: "Natural killer cells in the thymus. Studies in mice with severe combined immune deficiency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 796-803, 1990

Crowley, Inaba, Witmer-Pack, Steinman: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, 1989

Habu, Okumura, Diamantstein, Shevach: "Expression of interleukin 2 receptor on murine fetal thymocytes." in: European journal of immunology, Vol. 15, Issue 5, pp. 456-60, 1985

Malek, Schmidt, Shevach: "The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 4, pp. 2405-13, 1985