anti-Mouse (Murine) CD25 antibody for Immunoprecipitation

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Reactivity
Mouse (Murine)
626
451
145
22
16
8
7
6
6
5
4
4
1
1
1
1
Host
Rat
594
384
164
12
6
4
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
130
123
95
83
29
26
25
22
20
15
14
13
11
10
10
10
10
10
9
9
9
9
9
6
6
6
6
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
940
201
163
151
117
109
79
75
47
20
20
18
17
17
16
13
8
8
7
6
5
4
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN370891
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN370892 Func FACS IP Rat IgG2b Log in to see 3C7 15
1 ABIN2689117 CyTox IHC (fro) FACS IP ELISA Rat IgM, kappa Log in to see 7D4 15
1 ABIN2689118 BR FACS IP IHC Rat IgG1, lambda Log in to see PC61 13
1 ABIN2658949 FACS IP IHC PE-Cy5 Rat IgG1 lambda Log in to see PC61 12
1 ABIN370882 FACS IP Rat IgM Log in to see 7D4 8
1 ABIN370883 FACS IP Rat IgM Log in to see 7D4 8
1 ABIN1302515 FACS Func IHC (f) IP Rat IgG1 Log in to see PC61-5 8
1 ABIN1302926 FACS Func IHC (f) IP FITC Rat IgG1 Log in to see PC61-5 8
1 ABIN1302633 FACS Func IHC (f) IP APC Rat IgG1 Log in to see PC61-5 8
1 ABIN1303127 FACS Func IHC (f) IP PE Rat IgG1 Log in to see PC61-5 8
1 ABIN951247 IHC (fro) FACS IP Biotin Rat IgG1 Log in to see PC61-5-3 0
1 ABIN153558 CyTOF FACS IHC IHC (fro) IHC (p) IP Rat IgG1 Log in to see PC61-5-3 0
1 ABIN1302435 FACS Func IHC (f) IP Rat IgG1 Log in to see PC61-5 6
1 ABIN2479481 IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3 4
1 ABIN2479486 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3 4
1 ABIN5580827 FACS IP APC Rat IgM kappa Log in to see 7D4 0
1 ABIN5580828 FACS IP Biotin Rat IgM kappa Log in to see 7D4 0
1 ABIN5580829 FACS IP FITC Rat IgM kappa Log in to see 7D4 0
1 ABIN5580830 FACS IP PE Rat IgM kappa Log in to see 7D4 0
1 ABIN5580831 FACS IP Cy7,PE Rat IgM kappa Log in to see 7D4 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(626), (451), (145), (22), (16), (8), (7), (6), (6), (5), (4), (4), (1), (1), (1), (1)
Host Rat
(594), (384), (164), (12), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(130), (123), (95), (83), (29), (26), (25), (22), (20), (15), (14), (13), (11), (10), (10), (10), (10), (10), (9), (9), (9), (9), (9), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(940), (201), (163), (151), (117), (109), (79), (75), (47), (20), (20), (18), (17), (17), (16), (13), (8), (8), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 15 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN370891) Images
Specificity This antibody is specific to Low affinity alpha chain IL-2 Receptor (IL-2Ra), Mr 55 kDa. The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4. (Ref.2,11,12) Clone 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone. (Ref.2,13)
Characteristics Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen
Endotoxin Level Low Endotoxin
Clone 3C7
Isotype IgG2b
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN370891) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. (Ref.14) CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. (Ref.2,3,15) Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated. (Ref.4) CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. (Ref.5) It is expressed at a higher level on CD4+CD8+ thymocytes. (Ref.5,6) It is also expressed on cultured epidermal Langerhans cells. (Ref.7) The biochemical features of murine CD25 have been characterized in detail. (Ref.8-10)Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 16184
UniProt P01590
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN370891) Images back to top
Application Notes Flow Cytometry: Identification and enumeration of CD25+ (Ref.1-4)Immunoprecipitation. (Ref.1,2,8,10)Functional Assay: Inhibition of IL-2 or mitogen-induced T cell proliferation. (Ref.13)In vitro blocking of IL-2 binding. (Ref.2,12)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN370891) Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing no preservatives.
Preservative Without preservative
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD25 antibody (ABIN370891)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Chen, Ma, Young, Alt: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, 1995

Rolink, Grawunder, Winkler, Karasuyama, Melchers: "IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development." in: International immunology, Vol. 6, Issue 8, pp. 1257-64, 1995

Lorenzo, Jaulin, Vita, Froussard, Ferrara, Jankovic, Theze: "Structure-function study of the p55 subunit of murine IL-2 receptor by epitope mapping." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 9, pp. 2970-7, 1991

Saragovi, Malek: "Direct identification of the murine IL-2 receptor p55-p75 heterodimer in the absence of IL-2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 2, pp. 476-82, 1988

Moreau, Nabholz, Diamantstein, Malek, Shevach, Thèze: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, 1987

Steiner, Tschachler, Tani, Malek, Shevach, Holter, Knapp, Wolff, Stingl: "Interleukin 2 receptors on cultured murine epidermal Langerhans cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 1, pp. 155-9, 1986

Malek, Korty: "The murine interleukin 2 receptor. IV. Biochemical characterization." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 11, pp. 4092-8, 1986

Malek, Ashwell, Germain, Shevach, Miller: "The murine interleukin-2 receptor: biochemical structure and regulation of expression." in: Immunological reviews, Vol. 92, pp. 81-101, 1986

Malek, Schmidt, Shevach: "The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 4, pp. 2405-13, 1985

Andrew, Churilla, Malek, Braciale, Braciale: "Activation of virus specific CTL clones: antigen-dependent regulation of interleukin 2 receptor expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 2, pp. 920-5, 1985

Habu, Okumura, Diamantstein, Shevach: "Expression of interleukin 2 receptor on murine fetal thymocytes." in: European journal of immunology, Vol. 15, Issue 5, pp. 456-60, 1985

Ortega, Robb, Shevach, Malek: "The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1970-5, 1984

Malek, Ortega, Jakway, Chan, Shevach: "The murine IL 2 receptor. II. Monoclonal anti-IL 2 receptor antibodies as specific inhibitors of T cell function in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1976-82, 1984

Leonard, Depper, Crabtree, Rudikoff, Pumphrey, Robb, Krönke, Svetlik, Peffer, Waldmann: "Molecular cloning and expression of cDNAs for the human interleukin-2 receptor." in: Nature, Vol. 311, Issue 5987, pp. 626-31, 1984

Malek, Robb, Shevach: "Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin 2 receptor-ligand complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 18, pp. 5694-8, 1983