anti-Mouse (Murine) CD25 antibody for Immunoprecipitation

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Reactivity
Mouse (Murine)
643
476
172
23
19
15
9
7
6
6
5
4
4
4
3
1
Host
Rat
633
397
169
7
5
3
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
133
126
102
82
29
28
27
23
22
17
17
15
13
11
11
11
11
11
10
10
9
8
8
7
7
7
7
6
6
6
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA
989
225
178
155
129
124
81
78
55
30
20
20
17
17
16
13
8
8
7
6
5
4
2
2
1
1
1
1
Options
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Catalog No. ABIN2689117
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2689118 BR FACS IP IHC Rat IgG1, lambda Log in to see PC61 13
1 ABIN1302515 FACS Func IHC (f) IP Rat IgG1 Log in to see PC61-5 8
1 ABIN1302633 FACS Func IHC (f) IP APC Rat IgG1 Log in to see PC61-5 8
1 ABIN1302926 FACS Func IHC (f) IP FITC Rat IgG1 Log in to see PC61-5 8
1 ABIN1303127 FACS Func IHC (f) IP PE Rat IgG1 Log in to see PC61-5 8
1 ABIN1302435 FACS Func IHC (f) IP Rat IgG1 Log in to see PC61-5 6
1 ABIN370891 Func FACS IP Rat IgG2b Log in to see 3C7 15
1 ABIN370892 Func FACS IP Rat IgG2b Log in to see 3C7 15
1 ABIN2658949 FACS IP IHC PE-Cy5 Rat IgG1 lambda Log in to see PC61 12
1 ABIN370882 FACS IP Rat IgM Log in to see 7D4 8
1 ABIN370883 FACS IP Rat IgM Log in to see 7D4 8
1 ABIN951247 IHC (fro) FACS IP Biotin Rat IgG1 Log in to see PC61-5-3 0
1 ABIN153558 CyTOF FACS IHC IHC (fro) IHC (p) IP Rat IgG1 Log in to see PC61-5-3 0
1 ABIN2479481 IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3 4
1 ABIN2479486 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3 4
1 ABIN601656 FACS IHC IHC (fro) IP Rat IgG1 Log in to see 0
1 ABIN2609851 FACS IP Rat IgG2b Log in to see 3C7 0
1 ABIN2609852 FACS IP Rat IgM Log in to see 7D4 0
1 ABIN601654 FACS Func IHC IHC (fro) IP Rat IgG1 Log in to see 0
1 ABIN2609850 FACS IP Biotin Rat IgG2b Log in to see 3C7 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(643), (476), (172), (23), (19), (15), (9), (7), (6), (6), (5), (4), (4), (4), (3), (1)
Host Rat
(633), (397), (169), (7), (5), (3), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(133), (126), (102), (82), (29), (28), (27), (23), (22), (17), (17), (15), (13), (11), (11), (11), (11), (11), (10), (10), (9), (8), (8), (7), (7), (7), (7), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA
(989), (225), (178), (155), (129), (124), (81), (78), (55), (30), (20), (20), (17), (17), (16), (13), (8), (8), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1)
Pubmed 15 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN2689117) Images
Brand BD Pharmingen™
Characteristics The 7D4 antibody reacts with CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γ (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. no. 553068). The 3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by mAbs 7D4 and PC61 (Cat. no. 553866), and it blocks binding of IL-2 to CD25. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD25 - Purified - Clone 7D4 - Isotype Rat IgM, κ - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Clone 7D4
Isotype IgM, kappa
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN2689117) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background Synonyms: IL-2 Receptor α chain, p55
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN2689117) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN2689117) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD25 antibody (ABIN2689117)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, 2000

Takahashi, Tagami, Yamazaki, Uede, Shimizu, Sakaguchi, Mak, Sakaguchi: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, 2000

Chen, Ma, Young, Alt: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, 1995

Rolink, Grawunder, Winkler, Karasuyama, Melchers: "IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development." in: International immunology, Vol. 6, Issue 8, pp. 1257-64, 1995

Godfrey, Kennedy, Mombaerts, Tonegawa, Zlotnik: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, 1994

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Lorenzo, Jaulin, Vita, Froussard, Ferrara, Jankovic, Theze: "Structure-function study of the p55 subunit of murine IL-2 receptor by epitope mapping." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 9, pp. 2970-7, 1991

Pollard, Lipscomb: "Characterization of murine lung dendritic cells: similarities to Langerhans cells and thymic dendritic cells." in: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 159-67, 1990

Garni-Wagner, Witte, Tutt, Kuziel, Tucker, Bennett, Kumar: "Natural killer cells in the thymus. Studies in mice with severe combined immune deficiency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 796-803, 1990

Crowley, Inaba, Witmer-Pack, Steinman: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, 1989

Osawa, Josimovic-Alasevic, Diamantstein: "Interleukin 2 receptors are released by cells in vitro and in vivo. I. Detection of soluble IL 2 receptors in cell culture supernatants and in the serum of mice by an immunoradiometric assay." in: European journal of immunology, Vol. 16, Issue 4, pp. 467-9, 1986

Habu, Okumura, Diamantstein, Shevach: "Expression of interleukin 2 receptor on murine fetal thymocytes." in: European journal of immunology, Vol. 15, Issue 5, pp. 456-60, 1985

Malek, Schmidt, Shevach: "The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 4, pp. 2405-13, 1985

Ortega, Robb, Shevach, Malek: "The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1970-5, 1984

Malek, Robb, Shevach: "Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin 2 receptor-ligand complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 18, pp. 5694-8, 1983