anti-Rat (Rattus) CD25 antibody for Flow Cytometry
Recommended CD25 Antibody (supplied by: Log in to see )
- Antigen
-
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
show synonyms for this antigen- CD25
- IDDM10
- IL2R
- TCGFR
- IL2RAC
- IL-2RA
- Il2r
- Ly-43
- interleukin 2 receptor subunit alpha
- interleukin-2 receptor subunit alpha-like
- interleukin 2 receptor, alpha chain
- IL2RA
- Il2ra
- LOC100222957
- Epitope
-
Chain alpha
Alternatives - Reactivity
-
Rat (Rattus)
Alternatives - Host
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Mouse
Alternatives - Clonality
- Conjugate
-
This CD25 antibody is un-conjugated
Alternatives - Application
-
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA
Alternatives - Supplier
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- Supplier Product No.
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 13.403709 | ABIN120429 | EIA FACS IHC (fro) IHC (p) | Mouse | IgG1 | Log in to see | OX-39 | 3 | |||
| 13.403709 | ABIN2479468 | ELISA FACS IHC (fro) | Mouse | IgG1 | Log in to see | NDS 61 | 4 | |||
| 13.403709 | ABIN4896463 | FACS | PE | Mouse | IgG1 | AA 22-235 | Log in to see | 745520 | 0 | |
| 13.403709 | ABIN4896474 | FACS | PE | Goat | IgG | AA 22-236 | Log in to see | Polyclonal | 0 | |
| 13.403709 | ABIN2479465 | IHC (fro) FACS IHC (p) IP ELISA | Mouse | IgG1 | Log in to see | OX-39 | 4 | |||
| 13.403709 | ABIN120431 | EIA Func FACS IHC (fro) | Mouse | IgG1 | Log in to see | NDS 61 | 0 | |||
| 13.403709 | ABIN2663212 | FACS IP | PE | Mouse | IgG1 kappa | Log in to see | OX-39 | 3 | ||
| 13.403709 | ABIN2661002 | FACS | Biotin | Mouse | IgG1 kappa | Log in to see | OX-39 | 3 | ||
| 13.403709 | ABIN2657370 | ELISA (Detection) FACS IHC | Alexa Fluor 488 | Mouse | IgG1 kappa | Log in to see | OX-39 | 3 | ||
| 1 | ABIN2689119 | BR IHC (fro) FACS IP | Mouse | IgG1, kappa | Log in to see | OX | 4 | |||
| 1 | ABIN2689122 | FACS | Biotin | Mouse | IgG1, kappa | Log in to see | OX | 3 | ||
| 1 | ABIN120427 | EIA FACS IHC (fro) IHC (p) | Mouse | IgG1 | Log in to see | OX-39 | 3 | |||
| 1 | ABIN120430 | FACS | PE | Mouse | IgG1 | Log in to see | OX-39 | 1 | ||
| 1 | ABIN120428 | EIA FACS IHC (fro) IHC (p) IP | Mouse | IgG1 | Log in to see | OX-39 | 6 | |||
| 1 | ABIN120426 | EIA Func FACS IHC (fro) IHC (p) IP | Mouse | IgG1 | Log in to see | OX-39 | 6 | |||
| 1 | ABIN476836 | FACS IHC IHC (fro) | APC | Mouse | IgG1 | Log in to see | OX-39 | 0 | ||
| 1 | ABIN120425 | FACS | FITC | Mouse | IgG1 | Log in to see | OX-39 | 3 | ||
| 1 | ABIN320308 | FACS | FITC | Mouse | IgG1 | Log in to see | OX-39 | 3 | ||
| 1 | ABIN120434 | EIA FACS IHC (fro) | Mouse | IgG1 | Log in to see | NDS 61 | 3 | |||
| 1 | ABIN120433 | EIA FACS IHC (fro) | Mouse | IgG1 | Log in to see | NDS 61 | 1 |
General |
|
|---|---|
| Antigen | Interleukin 2 Receptor, alpha (IL2RA) Antibodies |
| Epitope | Chain alpha Alternatives |
| Reactivity | Rat (Rattus) Alternatives |
| Host | Mouse Alternatives |
| Clonality (Clone) | |
| Conjugate | This CD25 antibody is un-conjugated Alternatives |
| Application |
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA
Alternatives
|
|
4 references available |
| Supplier | Log in to see |
Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN4290997) Images |
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| Specificity | This antibody recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) ^(6). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. |
| Purification | Protein G purified |
| Immunogen | Stimulated Rat T cells |
| Clone | OX-39 |
| Isotype | IgG1 |
| Plasmids, Primers & others | |
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN4290997) Images back to top |
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| Antigen | |
| Alternative Name | CD25/IL-2 R alpha (IL2RA Antibody Abstract) |
| Background | Gene Symbol: IL2RA |
| Gene ID | 3559 |
| UniProt | P26897 |
| Pathways | JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation |
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN4290997) Images back to top |
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| Application Notes | Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500 |
| Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
| Restrictions | For Research Use only |
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN4290997) Images back to top |
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| Format | Liquid |
| Concentration | 1.0 mg/mL |
| Buffer |
PBS ( pH 7.4) Buffer contains: 0.09 % Sodium Azide |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Handling Advice | Avoid freeze-thaw cycles |
| Storage | 4 °C,-20 °C |
| Storage Comment | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
References for anti-CD25 antibody (ABIN4290997)Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top |
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| Product cited in: |
Crook, Hunt: "Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82." in: Developmental immunology, Vol. 4, Issue 4, pp. 235-46, 1996 Lazo, Klein-Szanto, Tsichlis: "T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications." in: Journal of virology, Vol. 64, Issue 8, pp. 3948-59, 1990 (Sample species: Rat (Rattus)). Further details: Flow Cytometry,Immunohistochemistry (Frozen Sections) Kampinga, Schuurman, Pol, Bartels, Broekhuizen, Vaessen, Tielen, Rozing, Blaauw, Roser: "Vascular thymus transplantation in rats. Technique, morphology, and function." in: Transplantation, Vol. 50, Issue 4, pp. 669-78, 1990 Bouchot, Anegon, Romaniuk, Jacques, Paineau, Soulillou: "Interleukin 2 receptor in rat heart allograft rejection." in: Transplantation, Vol. 48, Issue 6, pp. 918-22, 1990 |
ImagesProduct Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN4290997) back to top |
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| Supplier Images |
Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (OX-39) [ABIN2557811] - Rat...
Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [ABIN25578...
Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [ABIN25578...
Flow Cytometry: CD25/IL-2 R alpha Antibody (OX-39) [ABIN2557811] - Staining of Con A ...
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