anti-Rat (Rattus) CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Epitope
Chain alpha
23
21
19
18
15
15
15
13
13
13
7
6
6
6
6
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Rat (Rattus)
643
477
171
23
19
15
9
7
6
6
5
4
4
4
3
1
Host
Mouse
632
398
169
7
5
3
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
133
126
102
82
29
28
27
23
22
17
17
15
13
11
11
11
11
11
10
10
9
8
8
7
7
7
7
6
6
6
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA
989
224
178
155
129
124
81
77
55
30
20
20
17
17
16
13
8
8
7
6
5
4
2
2
1
1
1
1
1
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Catalog No. ABIN4290997
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.403709 ABIN120429 EIA FACS IHC (fro) IHC (p) Mouse IgG1 Log in to see OX-39 3
13.403709 ABIN2479468 ELISA FACS IHC (fro) Mouse IgG1 Log in to see NDS 61 4
13.403709 ABIN4896463 FACS PE Mouse IgG1 AA 22-235 Log in to see 745520 0
13.403709 ABIN4896474 FACS PE Goat IgG AA 22-236 Log in to see Polyclonal 0
13.403709 ABIN2479465 IHC (fro) FACS IHC (p) IP ELISA Mouse IgG1 Log in to see OX-39 4
13.403709 ABIN120431 EIA Func FACS IHC (fro) Mouse IgG1 Log in to see NDS 61 0
13.403709 ABIN2663212 FACS IP PE Mouse IgG1 kappa Log in to see OX-39 3
13.403709 ABIN2661002 FACS Biotin Mouse IgG1 kappa Log in to see OX-39 3
13.403709 ABIN2657370 ELISA (Detection) FACS IHC Alexa Fluor 488 Mouse IgG1 kappa Log in to see OX-39 3
1 ABIN2689119 BR IHC (fro) FACS IP Mouse IgG1, kappa Log in to see OX 4
1 ABIN2689122 FACS Biotin Mouse IgG1, kappa Log in to see OX 3
1 ABIN120427 EIA FACS IHC (fro) IHC (p) Mouse IgG1 Log in to see OX-39 3
1 ABIN120430 FACS PE Mouse IgG1 Log in to see OX-39 1
1 ABIN120428 EIA FACS IHC (fro) IHC (p) IP Mouse IgG1 Log in to see OX-39 6
1 ABIN120426 EIA Func FACS IHC (fro) IHC (p) IP Mouse IgG1 Log in to see OX-39 6
1 ABIN476836 FACS IHC IHC (fro) APC Mouse IgG1 Log in to see OX-39 0
1 ABIN120425 FACS FITC Mouse IgG1 Log in to see OX-39 3
1 ABIN320308 FACS FITC Mouse IgG1 Log in to see OX-39 3
1 ABIN120434 EIA FACS IHC (fro) Mouse IgG1 Log in to see NDS 61 3
1 ABIN120433 EIA FACS IHC (fro) Mouse IgG1 Log in to see NDS 61 1

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope Chain alpha
(23), (21), (19), (18), (15), (15), (15), (13), (13), (13), (7), (6), (6), (6), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(643), (477), (171), (23), (19), (15), (9), (7), (6), (6), (5), (4), (4), (4), (3), (1)
Host Mouse
(632), (398), (169), (7), (5), (3), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(133), (126), (102), (82), (29), (28), (27), (23), (22), (17), (17), (15), (13), (11), (11), (11), (11), (11), (10), (10), (9), (8), (8), (7), (7), (7), (7), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA
(989), (224), (178), (155), (129), (124), (81), (77), (55), (30), (20), (20), (17), (17), (16), (13), (8), (8), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN4290997) Images
Specificity This antibody recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) ^(6). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts.
Purification Protein G purified
Immunogen Stimulated Rat T cells
Clone OX-39
Isotype IgG1
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN4290997) Images back to top
Antigen
Alternative Name CD25/IL-2 R alpha (IL2RA Antibody Abstract)
Background Gene Symbol: IL2RA
Gene ID 3559
UniProt P26897
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN4290997) Images back to top
Application Notes Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN4290997) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CD25 antibody (ABIN4290997)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Crook, Hunt: "Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82." in: Developmental immunology, Vol. 4, Issue 4, pp. 235-46, 1996

Lazo, Klein-Szanto, Tsichlis: "T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications." in: Journal of virology, Vol. 64, Issue 8, pp. 3948-59, 1990 (Sample species: Rat (Rattus)). Further details: Flow Cytometry,Immunohistochemistry (Frozen Sections)

Kampinga, Schuurman, Pol, Bartels, Broekhuizen, Vaessen, Tielen, Rozing, Blaauw, Roser: "Vascular thymus transplantation in rats. Technique, morphology, and function." in: Transplantation, Vol. 50, Issue 4, pp. 669-78, 1990

Bouchot, Anegon, Romaniuk, Jacques, Paineau, Soulillou: "Interleukin 2 receptor in rat heart allograft rejection." in: Transplantation, Vol. 48, Issue 6, pp. 918-22, 1990

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN4290997) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (Chain alpha) antibody (ABIN4290997) Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (OX-39) [ABIN2557811] - Rat...
Immunofluorescence (IF) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (Chain alpha) antibody (ABIN4290997) Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [ABIN25578...
Immunofluorescence (IF) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (Chain alpha) antibody (ABIN4290997) Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [ABIN25578...
Flow Cytometry (FACS) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (Chain alpha) antibody (ABIN4290997) Flow Cytometry: CD25/IL-2 R alpha Antibody (OX-39) [ABIN2557811] - Staining of Con A ...