We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD25 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
|Supplier||Log in to see|
Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling Images
|Specificity||This antibody recognises the CD25 cell surface antigen, a 55 kDa molecules that is the low affinity alpha chain of the IL-2 receptor. Clone NDS61 blocks IL-2 induced cell proliferation.|
|Purification||Ion exchange chromatography|
|Immunogen||Rat interleukin-2 receptor. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.|
|Plasmids, Primers & others|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling Images back to top
|Alternative Name||CD25 / IL2RA (IL2RA Antibody Abstract)|
|Background||The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55|
|Pathways||JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Flow cytometry: 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Functional Assays. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
|Buffer||PBS, pH 7.4|
|Handling Advice||Avoid repeated freezing and thawing.|
ImagesProduct Details anti-CD25 Antibody Target Details CD25 Application Details Handling back to top