anti-Rat (Rattus) CD25 antibody for Functional Studies

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Reactivity
Rat (Rattus)
626
452
144
22
16
8
7
6
6
5
4
4
1
1
1
1
Host
Mouse
593
385
164
12
6
4
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
130
123
95
83
29
26
25
22
20
15
14
13
11
10
10
10
10
10
9
9
9
9
9
6
6
6
6
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
940
201
163
152
117
109
78
75
47
20
20
18
17
17
16
13
8
7
7
6
5
4
2
2
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN120431
$ 1,149.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN120426 EIA Func FACS IHC (fro) IHC (p) IP Mouse IgG1 Log in to see OX-39 6
1 ABIN2479475 Func IHC (fro) FACS IHC (p) IP ELISA Mouse IgG1 Log in to see OX-39 4
1 ABIN217773 ELISA FACS Func IHC IHC (fro) IHC (p) IP Mouse IgG1 Log in to see OX-39 0
1 ABIN210184 ELISA FACS Func IHC IHC (fro) Mouse IgG1 Log in to see NDS 61 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Rat (Rattus)
(626), (452), (144), (22), (16), (8), (7), (6), (6), (5), (4), (4), (1), (1), (1), (1)
Host Mouse
(593), (385), (164), (12), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(130), (123), (95), (83), (29), (26), (25), (22), (20), (15), (14), (13), (11), (10), (10), (10), (10), (10), (9), (9), (9), (9), (9), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(940), (201), (163), (152), (117), (109), (78), (75), (47), (20), (20), (18), (17), (17), (16), (13), (8), (7), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Specificity This antibody recognises the CD25 cell surface antigen, a 55 kDa molecules that is the low affinity alpha chain of the IL-2 receptor. Clone NDS61 blocks IL-2 induced cell proliferation.
Purification Ion exchange chromatography
Immunogen Rat interleukin-2 receptor. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone NDS 61
Isotype IgG1
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 25704
NCBI Accession NP_037295
UniProt P26897
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes Flow cytometry: 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Functional Assays. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (ABIN120431) Staining of Con A activated rat spleen cells with MOUSE ANTI RAT CD25: AZIDE FREE