anti-Rat (Rattus) CD25 antibody for Neutralization

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Epitope
AA 116-122, Extracellular Domain
23
19
15
15
13
13
12
10
8
7
7
6
6
5
5
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
625
451
144
22
16
8
7
6
6
5
4
4
1
1
1
1
Host
Rabbit
594
385
163
12
6
4
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
130
123
95
83
29
26
25
22
20
15
14
13
11
10
10
10
10
10
9
9
9
9
9
6
6
6
6
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Peptide (BP), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
940
200
162
152
116
109
79
75
48
20
20
17
17
16
16
13
8
8
7
6
5
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN4291069
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2224473 FACS Neut IHC ELISA Mouse IgG1 Log in to see 3H1161 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 116-122, Extracellular Domain
(23), (19), (15), (15), (13), (13), (12), (10), (8), (7), (7), (6), (6), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(625), (451), (144), (22), (16), (8), (7), (6), (6), (5), (4), (4), (1), (1), (1), (1)
Host Rabbit
(594), (385), (163), (12), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(130), (123), (95), (83), (29), (26), (25), (22), (20), (15), (14), (13), (11), (10), (10), (10), (10), (10), (9), (9), (9), (9), (9), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Peptide (BP), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
(940), (200), (162), (152), (116), (109), (79), (75), (48), (20), (20), (17), (17), (16), (16), (13), (8), (8), (7), (6), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Specificity Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit - binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R ( alpha beta yc) - T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
Characteristics Recombinant Monoclonal Antibody
Purification Protein A or G purified
Immunogen CD25
Clone Basiliximab
Isotype IgG
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD25/IL-2 R alpha (IL2RA Antibody Abstract)
Background Gene Symbol: IL2RA
Gene ID 3559
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes Western Blot 1:100 - 1:2000, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Blocking/Neutralizing 50 - 200 Molar excess, Immunofluorescence 1:10 - 1:500, CyTOF-ready
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Format Liquid
Buffer PBS
Buffer contains: 0.02 % Proclin 300
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.