anti-Human CISH antibody for Western Blotting

Recommended CISH Antibody

Antigen
Cytokine Inducible SH2-Containing Protein (CISH) Antibodies
  • CISH
  • cis
  • cish.a
  • ptk9l
  • twf1l
  • wu:fk57g04
  • zgc:154062
  • zgc:91817
  • g18
  • socs
  • cis-1
  • cish
  • BACTS2
  • CIS
  • CIS-1
  • G18
  • SOCS
  • AI385595
  • CIS1
  • Cis
  • F17
  • F23
  • cytokine inducible SH2 containing protein
  • cytokine inducible SH2-containing protein
  • CISH
  • cish
  • Cish
Epitope
C-Term
6
4
3
2
2
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
53
33
29
4
3
3
3
2
2
2
1
Host
Rabbit
54
2
Clonality
Polyclonal
Conjugate
This CISH antibody is un-conjugated
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
32
10
6
6
6
3
2
2
1
Options

Available images

Catalog No. ABIN2854656
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
17.452406 ABIN928660 WB Rabbit N-Term Polyclonal 0
13.594599 ABIN2137676 ICC IHC (p) WB Rabbit IgG C-Term Polyclonal 0
13.594599 ABIN6138639 WB Rabbit Polyclonal 0
10.857807 ABIN3044172 ICC IHC (p) WB Rabbit IgG AA 241-258, C-Term Polyclonal 0
10.857807 ABIN514421 IF WB Rabbit AA 1-258 Polyclonal 0
10.857807 ABIN514420 IF WB Mouse AA 1-258 Polyclonal 0
10.857807 ABIN3030694 ICC IHC (p) WB Rabbit IgG AA 241-258 Polyclonal 0
7.857807 ABIN2785100 WB Rabbit N-Term Polyclonal 0
7.857807 ABIN5013433 ICC IHC WB Rabbit IgG AA 1-258 Polyclonal 0
7.857807 ABIN6000418 IF/ICC IHC IP WB Rabbit AA 1-258 Polyclonal 0
4.857807 ABIN6260845 ELISA WB Rabbit IgG Polyclonal 0
4.857807 ABIN1980302 IHC WB Rabbit IgG Polyclonal 0
4 ABIN6746363 WB Rabbit AA 78-127 Polyclonal 0
2.7452528 ABIN440988 ICC IF IHC IHC (p) WB Rabbit IgG C-Term Polyclonal 0
1 ABIN2619282 ICC IHC IHC (p) WB Rabbit IgG AA 241-258 Polyclonal 0
1 ABIN217065 ELISA WB Rabbit C-Term Polyclonal 0
1 ABIN2233951 IC IHC WB Rabbit IgG C-Term Polyclonal 0
1 ABIN594315 IF WB Mouse AA 1-258 Polyclonal 0
1 ABIN6759616 IHC WB Rabbit IgG Polyclonal 0
1 ABIN2595181 WB Rabbit Internal Region Polyclonal 0

General

Antigen Cytokine Inducible SH2-Containing Protein (CISH) Antibodies
Epitope C-Term
(6), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(53), (33), (29), (4), (3), (3), (3), (2), (2), (2), (1)
Host Rabbit
(54), (2)
Clonality
Polyclonal
Conjugate This CISH antibody is un-conjugated
(3), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(32), (10), (6), (6), (6), (3), (2), (2), (1)
Supplier

Product Details anti-CISH Antibody

Target Details CISH Application Details Handling Images
Cross-Reactivity Mouse (Murine), Chicken, Rhesus Monkey, Cow (Bovine)
Cross-Reactivity (Details) Mouse (100 %), Chicken (85 %), Rhesus Monkey (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to CIS (cytokine inducible SH2-containing protein)
CIS antibody [C2C3], C-term
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CIS. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details CISH

Product Details anti-CISH Antibody Application Details Handling Images back to top
Antigen
Alternative Name CIS (CISH Antibody Abstract)
Background The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight 29 kDa
Gene ID 1154
Pathways JAK-STAT Signaling, Response to Growth Hormone Stimulus

Application Details

Product Details anti-CISH Antibody Target Details CISH Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*
Comment

Positive Control: HeLa , PC-12 , Rat2

Restrictions For Research Use only

Handling

Product Details anti-CISH Antibody Target Details CISH Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-CISH Antibody Target Details CISH Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Cytokine Inducible SH2-Containing Protein (CISH) (C-Term) antibody (ABIN2854656) IHC-P Image Immunohistochemical analysis of paraffin-embedded human lung cancer, usin...
Immunofluorescence (IF) image for anti-Cytokine Inducible SH2-Containing Protein (CISH) (C-Term) antibody (ABIN2854656) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using CIS, a...
Western Blotting (WB) image for anti-Cytokine Inducible SH2-Containing Protein (CISH) (C-Term) antibody (ABIN2854656) WB Image Sample (1 ug of whole cell lysate) A: HeLa 10% SDS PAGE antibody diluted at ...
Western Blotting (WB) image for anti-Cytokine Inducible SH2-Containing Protein (CISH) (C-Term) antibody (ABIN2854656) WB Image CIS antibody [C2C3], C-term detects CISH protein by Western blot analysis. A...