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|Antigen||Ciliary Neurotrophic Factor (CNTF) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CNTF antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Product Details anti-CNTF AntibodyTarget Details CNTF Application Details Handling Images
|Specificity||This antibody recognises Rat CNTF.|
|Immunogen||Highly purified recombinant CNTF.|
Target Details CNTFProduct Details anti-CNTF Antibody Application Details Handling Images back to top
|Alternative Name||CNTF (CNTF Antibody Abstract)|
|Background||CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity.Synonyms: Ciliary Neurotrophic Factor|
|Research Area||Stem Cells|
Application DetailsProduct Details anti-CNTF Antibody Target Details CNTF Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat CNTF (0.15 ng/mL), a concentration of 0.002-0.005 μg/mL of this antibody is required. ELISAIndirect: To detect Rat CNTF by indirect ELISA (using 100 l/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Rat CNTF. Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 l/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Rat CNTF as a detection antibody, allowsthe detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western BlotTo detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CNTF Antibody Target Details CNTF Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|