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|Antigen||Erythropoietin (EPO) Antibodies|
|Conjugate||This EPO antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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Product Details anti-EPO AntibodyTarget Details EPO Application Details Handling References for anti-EPO antibody (ABIN112633) Images
|Specificity||This antibody reacts with Erythropoietin, the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological levels of circulating erythrocyte mass. It is often used as a drug to treat anemia.|
|Purification||Affinity Chromatography on Protein A|
|Immunogen||CHO cell-derived, recombinant Human Erythropoietin.|
Target Details EPOProduct Details anti-EPO Antibody Application Details Handling References for anti-EPO antibody (ABIN112633) Images back to top
|Alternative Name||Erythropoietin / EPO (EPO Antibody Abstract)|
|Background||Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.Synonyms: Epoetin|
|Pathways||JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity|
Application DetailsProduct Details anti-EPO Antibody Target Details EPO Handling References for anti-EPO antibody (ABIN112633) Images back to top
ELISA: 0.5-1 μg/mL. Western Blot: 1-2 μg/mL (Reducing and Non-Reducing Conditions). Functional Assays: The ND50 was determined to be approximately 1-3 μg/mL, in thepresence of 0.3 units/mL of rhEpo, using a Human factor dependant cell line. Immunohistochemistry on Paraffin Sections: 15 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EPO Antibody Target Details EPO Application Details References for anti-EPO antibody (ABIN112633) Images back to top
|Buffer||PBS, pH 7.2, 0.09 % Sodium Azide, 5 % Trehalose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-EPO antibody (ABIN112633)Product Details anti-EPO Antibody Target Details EPO Application Details Handling Images back to top
Krystal: "A simple microassay for erythropoietin based on 3H-thymidine incorporation into spleen cells from phenylhydrazine treated mice." in: Experimental hematology, Vol. 11, Issue 7, pp. 649-60, 1983
Kessler: "Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 115, Issue 6, pp. 1617-24, 1976