anti-Mouse (Murine) FYN antibody for Western Blotting

Recommended FYN Antibody (supplied by: Log in to see )

Antigen
FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
  • im:6908851
  • si:dkey-119m7.5
  • zgc:109996
  • c-fyn
  • cfyn-a
  • SLK
  • SYN
  • p59-FYN
  • PKC
  • fyn
  • zgc:86720
  • AI448320
  • AW552119
  • FYN proto-oncogene, Src family tyrosine kinase
  • FYN proto-oncogene, Src family tyrosine kinase S homeolog
  • FYN proto-oncogene, Src family tyrosine kinase b
  • FYN proto-oncogene, Src family tyrosine kinase L homeolog
  • FYN proto-oncogene, Src family tyrosine kinase a
  • Fyn proto-oncogene
  • FYN
  • fyn.S
  • fynb
  • fyn.L
  • Fyn
  • fyna
Epitope
AA 7-176
38
22
16
12
11
11
11
7
6
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
236
140
58
10
8
7
5
5
5
5
4
3
2
1
Host
Mouse
151
87
20
Clonality
Monoclonal
Conjugate
This FYN antibody is un-conjugated
9
9
8
7
7
7
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
241
140
83
49
31
28
27
25
7
4
2
2
2
2
2
Options
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Catalog No. ABIN94311
$ 428.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.556355 ABIN969160 ELISA WB Mouse IgG1 Log in to see 2H8 3
23.056355 ABIN3182518 ELISA IHC WB Rabbit IgG pTyr530 Log in to see Polyclonal 0
20.056355 ABIN3184708 ELISA WB Rabbit IgG Ser400 Log in to see Polyclonal 0
17.752602 ABIN966167 ELISA WB Mouse IgG1 Log in to see 2H8 2
16.803753 ABIN6256757 ELISA ICC IF IHC WB Rabbit IgG pTyr530 Log in to see Polyclonal 0
13.803753 ABIN6269809 ELISA IHC WB Rabbit IgG pTyr213, pTyr214 Log in to see Polyclonal 0
13.252602 ABIN4901570 ELISA WB Mouse IgG2b Log in to see 4B8-E7-A8 0
13.252602 ABIN3184707 ELISA WB Rabbit IgG Ser396 Log in to see Polyclonal 0
13.252602 ABIN4901569 ELISA WB Mouse IgG1 Log in to see 2H8 0
13.252602 ABIN634345 WB Rabbit N-Term Log in to see Polyclonal 0
13.252602 ABIN2141143 IHC ELISA WB Rabbit IgG pTyr530 Log in to see Polyclonal 0
10.803753 ABIN392098 FACS IHC (p) WB Rabbit Ig Fraction AA 13-43, N-Term Log in to see Polyclonal 0
10.803753 ABIN6261837 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10.803753 ABIN3031009 FACS IHC ELISA WB Rabbit Ig Fraction AA 13-43 Log in to see Polyclonal 0
10.803753 ABIN1846352 ELISA WB Mouse IgG1 Log in to see 0
10.803753 ABIN3003445 FACS IHC WB Rabbit IgG Log in to see Polyclonal 0
10.803753 ABIN1847258 IHC ELISA WB Rabbit pTyr530 Log in to see Polyclonal 0
10 ABIN5534198 FACS IHC (p) WB Rabbit Ig Fraction AA 13-43, N-Term Log in to see Polyclonal 0
9.283474 ABIN4312914 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
7.803753 ABIN2786269 WB Rabbit Middle Region Log in to see Polyclonal 0

General

Antigen FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
Epitope AA 7-176
(38), (22), (16), (12), (11), (11), (11), (7), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(236), (140), (58), (10), (8), (7), (5), (5), (5), (5), (4), (3), (2), (1)
Host Mouse
(151), (87), (20)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FYN antibody is un-conjugated
(9), (9), (8), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(241), (140), (83), (49), (31), (28), (27), (25), (7), (4), (2), (2), (2), (2), (2)
Pubmed 10 references available
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Product Details anti-FYN Antibody

Target Details FYN Application Details Handling References for anti-FYN antibody (ABIN94311) Images
Specificity The antibody FYN-01 reacts with Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase.
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Bacterially expressed recombinant fragment of human Fyn (aa 7-176).
Clone FYN-01
Isotype IgG2b
Plasmids, Primers & others

Target Details FYN

Product Details anti-FYN Antibody Application Details Handling References for anti-FYN antibody (ABIN94311) Images back to top
Antigen
Alternative Name FYN (FYN Antibody Abstract)
Background Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.
Pathways JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-FYN Antibody Target Details FYN Handling References for anti-FYN antibody (ABIN94311) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-FYN Antibody Target Details FYN Application Details References for anti-FYN antibody (ABIN94311) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-FYN antibody (ABIN94311)

Product Details anti-FYN Antibody Target Details FYN Application Details Handling Images back to top
Product cited in:

Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, 2007

Background publications

Rivera, Olivera: "A current understanding of Fc epsilon RI-dependent mast cell activation." in: Current allergy and asthma reports, Vol. 8, Issue 1, pp. 14-20, 2008

Makinodan, Manabe, Makinodan, Yamauchi, Matsuyoshi, Sakumura, Tatsumi, Wanaka: "A novel role for Fyn: change in sphere formation ability in murine embryonic stem cells." in: Neuroscience, Vol. 147, Issue 1, pp. 1-4, 2007

Samayawardhena, Kapur, Craig: "Involvement of Fyn kinase in Kit and integrin-mediated Rac activation, cytoskeletal reorganization, and chemotaxis of mast cells." in: Blood, Vol. 109, Issue 9, pp. 3679-86, 2007

Filby, Seddon, Kleczkowska, Salmond, Tomlinson, Smida, Lindquist, Schraven, Zamoyska: "Fyn regulates the duration of TCR engagement needed for commitment to effector function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 7, pp. 4635-44, 2007

Wu, Hsu, Gleichman, Baconguis, Coulter, Lynch: "Fyn-mediated phosphorylation of NR2B Tyr-1336 controls calpain-mediated NR2B cleavage in neurons and heterologous systems." in: The Journal of biological chemistry, Vol. 282, Issue 28, pp. 20075-87, 2007

Hattori, Uchino, Isosaka, Maekawa, Iyo, Sato, Kohsaka, Yagi, Yuasa: "Fyn is required for haloperidol-induced catalepsy in mice." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7129-35, 2006

Talmor-Cohen, Tomashov-Matar, Tsai, Kinsey, Shalgi: "Fyn kinase-tubulin interaction during meiosis of rat eggs." in: Reproduction (Cambridge, England), Vol. 128, Issue 4, pp. 387-93, 2004

Utting, Priatel, Teh, Teh: "p59fyn (Fyn) promotes the survival of anergic CD4-CD8- alpha beta TCR+ cells but negatively regulates their proliferative response to antigen stimulation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 3, pp. 1540-6, 2001

Images

Product Details anti-FYN Antibody Target Details FYN Application Details Handling References for anti-FYN antibody (ABIN94311) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats...
Immunofluorescence (IF) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunofluorescence staining of Fyn in human primary fibroblasts using anti-Fyn (; gre...
Immunofluorescence (IF) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunofluorescence staining of Fyn in human HeLa cell line using anti-Fyn
Immunofluorescence (IF) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunofluorescence staining of Fyn in murine transformed fibroblasts using anti-Fyn (...