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|Antigen||Interleukin 4 (IL4) Antibodies|
|Reactivity||Cow (Bovine) Alternatives|
|Conjugate||This IL-4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
|2 references available|
Product Details anti-IL-4 AntibodyTarget Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN120253) Images
|Specificity||This antibody is specific for interleukin 4 (IL-4). This reagent may be used as a capture antibody in ELISA assays for bovine IL-4 in combination with SM2242B as a detection reagent, and recombinant bovine IL-4 (SA1007) as a standard.|
|Purification||Affinity chromatography on Protein G|
|Immunogen||Recombinant bovine IL-4. Spleen cells form immunised BALB/c mice were fused with cells of the SP2/O myeloma cell line.|
|Plasmids, Primers & others|
Target Details IL-4Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 antibody (ABIN120253) Images back to top
|Alternative Name||Interleukin-4 / IL4 (IL4 Antibody Abstract)|
|Background||IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra|
|Pathways||JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation|
Application DetailsProduct Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 antibody (ABIN120253) Images back to top
Flow Cytometry. ELISA: 2 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 antibody (ABIN120253) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-IL-4 antibody (ABIN120253)Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Abbott, Palmer, Kegerreis, Hetrick, Howard, Hope, Brown: "Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 6702-15, 2005
Hope, Kwong, Thom, Sopp, Mwangi, Brown, Palmer, Wattegedera, Entrican, Howard: "Development of detection methods for ruminant interleukin (IL)-4." in: Journal of immunological methods, Vol. 301, Issue 1-2, pp. 114-23, 2005