anti-Cow (Bovine) IL-4 antibody for Enzyme Immunoassay

Recommended IL-4 Antibody

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Reactivity
Cow (Bovine)
311
168
49
25
21
21
16
14
13
12
9
8
6
6
6
4
3
2
1
Host
Mouse
280
143
135
8
7
4
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
73
40
30
25
18
8
4
3
2
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
325
304
154
140
51
50
32
23
22
22
21
20
19
11
6
6
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Options
Catalog No. ABIN120253
$ 676.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
1 ABIN317377 EIA Mouse IgG2a CC303 0

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Cow (Bovine)
(311), (168), (49), (25), (21), (21), (16), (14), (13), (12), (9), (8), (6), (6), (6), (4), (3), (2), (1)
Host Mouse
(280), (143), (135), (8), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(73), (40), (30), (25), (18), (8), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
(325), (304), (154), (140), (51), (50), (32), (23), (22), (22), (21), (20), (19), (11), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier

Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN120253) Images
Specificity This antibody is specific for interleukin 4 (IL-4). This reagent may be used as a capture antibody in ELISA assays for bovine IL-4 in combination with SM2242B as a detection reagent, and recombinant bovine IL-4 (SA1007) as a standard.
Purification Affinity chromatography on Protein G
Immunogen Recombinant bovine IL-4. Spleen cells form immunised BALB/c mice were fused with cells of the SP2/O myeloma cell line.
Clone CC313
Isotype IgG1
Plasmids, Primers & others

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 antibody (ABIN120253) Images back to top
Antigen
Alternative Name Interleukin-4 / IL4 (IL4 Antibody Abstract)
Background IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 280824
NCBI Accession NP_776346
UniProt P30367
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 antibody (ABIN120253) Images back to top
Application Notes Flow Cytometry. ELISA: 2 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 antibody (ABIN120253) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL-4 antibody (ABIN120253)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Abbott, Palmer, Kegerreis, Hetrick, Howard, Hope, Brown: "Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 6702-15, 2005

Hope, Kwong, Thom, Sopp, Mwangi, Brown, Palmer, Wattegedera, Entrican, Howard: "Development of detection methods for ruminant interleukin (IL)-4." in: Journal of immunological methods, Vol. 301, Issue 1-2, pp. 114-23, 2005