anti-Human IL-4 antibody for ELISpot

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Reactivity
Human
338
228
54
26
25
22
21
16
15
13
12
9
8
6
6
6
4
3
2
1
Host
Mouse
296
204
179
10
6
4
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
90
51
38
32
20
8
8
8
7
6
4
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
ELISpot, ELISA
374
363
241
158
57
53
38
30
24
23
22
22
22
12
8
8
5
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN476578
$ 346.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1107851 ELISA (Detection) ELISpot Biotin Mouse IgG1 Log in to see B-G28 0
1 ABIN1107848 ELISA (Capture) ELISpot Mouse IgG1 Log in to see B-R14 0
1 ABIN1724550 ELISpot ELISA Mouse IgG1 Log in to see B-R14 0
1 ABIN2748987 ELISpot Neut IHC ELISA Mouse IgG1 Log in to see 5B5 0
1 ABIN1448855 ELISpot Log in to see 0

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Human
(338), (228), (54), (26), (25), (22), (21), (16), (15), (13), (12), (9), (8), (6), (6), (6), (4), (3), (2), (1)
Host Mouse
(296), (204), (179), (10), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(90), (51), (38), (32), (20), (8), (8), (8), (7), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISpot, ELISA
(374), (363), (241), (158), (57), (53), (38), (30), (24), (23), (22), (22), (22), (12), (8), (8), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Purification Affinity purified
Immunogen Human IL4
Clone 8D4-8
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL4 (IL4 Antibody Abstract)
Background Name/Gene ID: IL4
Family: Hormone

Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4
Gene ID 3565
UniProt P05112
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Approved: ELISA, ELISPOT

Usage: A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate. The fluorochrome-conjugated 8D4-8 antibody is offered in 2 formats:- μg size: has been tested by intracellular staining for flow cytometric analysis. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining for flow cytometric analysis. This can be used at 20 μL per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS,  pH 7.2, 150 mM sodium chloride, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freezing and store undiluted.
Storage 4 °C
Storage Comment Store at 4°C, avoid freeze-thaw cycles.

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
 image for anti-Interleukin 4 (IL4) antibody (ABIN476578) anti-Interleukin 4 (IL4) antibody