anti-Human IL-4 antibody for Flow Cytometry

Recommended IL-4 Antibody

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Epitope
AA 122-150
32
23
10
9
7
7
7
6
5
5
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
310
167
49
25
22
21
16
14
13
12
9
8
6
6
6
4
3
2
1
Host
Rabbit
279
143
136
8
7
4
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
73
40
30
25
18
8
4
3
2
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
324
303
154
139
51
50
32
23
22
22
21
21
19
11
6
6
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
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Available images

Catalog No. ABIN3031478
$ 452.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
15.319508 ABIN3002277 FACS IHC WB Rabbit IgG Polyclonal 0
13.819508 ABIN4896834 FACS Fluorescein Mouse IgG1 AA 25-153 3007 3
13.819508 ABIN4896836 FACS PE Mouse IgG1 AA 25-153 3007 3
13.448064 ABIN1383959 FACS PE Mouse IgG1 B-G28 0
13.448064 ABIN2144132 Func FACS Rat IgG1 kappa 11B11 0
13.448064 ABIN2144142 FACS PE Mouse IgG1 kappa 8D4-8 0
13.448064 ABIN2144145 FACS APC Mouse IgG1 kappa 8D4-8 0
12.319508 ABIN2658787 FACS APC Mouse IgG1 kappa 8D4-8 11
12.319508 ABIN2659592 FACS PE-Cy7 Rat IgG1 kappa MP4-25D2 10
12.319508 ABIN2659777 FACS IF IHC PE/Dazzle™ 594 Rat IgG1 kappa MP4-25D2 10
12.319508 ABIN2657546 FACS Alexa Fluor 488 Mouse IgG1 kappa 8D4-8 10
12.319508 ABIN2657960 FACS Alexa Fluor 647 Mouse IgG1 kappa 8D4-8 10
12.319508 ABIN2657961 FACS Alexa Fluor 647 Rat IgG1 kappa MP4-25D2 10
10 ABIN5531473 FACS IHC (p) WB Rabbit Ig Fraction AA 122-150, C-Term Polyclonal 0
9.319508 ABIN2663795 FACS PE Rat IgG1 kappa MP4-25D2 10
9.319508 ABIN2661947 FACS FITC Rat IgG1 kappa MP4-25D2 10
9.319508 ABIN2657547 FACS Alexa Fluor 488 Rat IgG1 kappa MP4-25D2 10
9.319508 ABIN2660448 FACS PerCP-Cy5.5 Rat IgG1 kappa MP4-25D2 10
9.319508 ABIN2658789 FACS APC Rat IgG1 kappa MP4-25D2 10
7 ABIN474012 FACS PE Mouse IgG1, kappa 8D4-8 0

General

Antigen Interleukin 4 (IL4) Antibodies
Epitope AA 122-150
(32), (23), (10), (9), (7), (7), (7), (6), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(310), (167), (49), (25), (22), (21), (16), (14), (13), (12), (9), (8), (6), (6), (6), (4), (3), (2), (1)
Host Rabbit
(279), (143), (136), (8), (7), (4)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(73), (40), (30), (25), (18), (8), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(324), (303), (154), (139), (51), (50), (32), (23), (22), (22), (21), (21), (19), (11), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Supplier

Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Cross-Reactivity (Details) Expected species reactivity: Primate
Purification Antigen affinity purified
Immunogen A portion of amino acids 122-150 from the human protein was used as the immunogen for this IL-4 antibody.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Background IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.
UniProt P05112
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Titration of the IL-4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Liquid
Buffer In 1X PBS pH 7.4 with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot the IL-4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) (Left) Western blot analysis of IL-4 antibody and WiDr lysate; (Right) Western blot a...
Flow Cytometry (FACS) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) IL-4 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a ...
Immunohistochemistry (IHC) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) IL-4 antibody IHC analysis in formalin fixed and paraffin embedded tonsil
Western Blotting (WB) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) (Left) Western blot analysis of IL-4 antibody and WiDr lysate
Flow Cytometry (FACS) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) IL-4 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a ...