anti-Human IL-4 antibody for Flow Cytometry

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Reactivity
Human
407
230
56
33
25
22
21
16
15
14
13
12
9
8
6
6
6
5
3
2
1
Host
Rat
296
243
219
10
6
4
Clonality
Monoclonal
Conjugate
This IL-4 antibody is conjugated to PE
92
55
41
36
23
13
13
10
9
9
8
7
7
7
7
7
7
5
5
4
4
3
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
426
390
281
176
58
55
45
39
38
38
25
22
22
13
8
8
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2663795
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.363007 ABIN2661947 FACS FITC Rat IgG1 kappa Log in to see MP4-25D2 10
13.363007 ABIN2658787 FACS APC Mouse IgG1 kappa Log in to see 8D4-8 11
13.363007 ABIN4896834 FACS Fluorescein Mouse IgG1 AA 25-153 Log in to see 3007 3
13.363007 ABIN4896836 FACS PE Mouse IgG1 AA 25-153 Log in to see 3007 3
13.363007 ABIN2659777 FACS IF IHC PE/Dazzle™ 594 Rat IgG1 kappa Log in to see MP4-25D2 10
13.363007 ABIN2659592 FACS PE-Cy7 Rat IgG1 kappa Log in to see MP4-25D2 10
13.363007 ABIN2660448 FACS PerCP-Cy5.5 Rat IgG1 kappa Log in to see MP4-25D2 10
13.363007 ABIN2657546 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see 8D4-8 10
13.363007 ABIN2657547 FACS Alexa Fluor 488 Rat IgG1 kappa Log in to see MP4-25D2 10
13.363007 ABIN2657960 FACS Alexa Fluor 647 Mouse IgG1 kappa Log in to see 8D4-8 10
13.363007 ABIN2657961 FACS Alexa Fluor 647 Rat IgG1 kappa Log in to see MP4-25D2 10
13.363007 ABIN2658789 FACS APC Rat IgG1 kappa Log in to see MP4-25D2 10
13.363007 ABIN651434 FACS IHC (p) WB Rabbit Ig Fraction AA 122-150, C-Term Log in to see Polyclonal 1
13.363007 ABIN3031478 FACS IHC ELISA WB Rabbit Ig Fraction AA 122-150 Log in to see Polyclonal 0
1 ABIN119373 EIA FACS IHC (fro) WB Rat IgG1 Log in to see MP4-25D2 6
1 ABIN2661231 FACS Biotin Rat IgG1 kappa Log in to see MP4-25D2 10
1 ABIN1107850 FACS PE Mouse IgG1 Log in to see 8F-12 9
1 ABIN1107847 FACS Mouse IgG1 Log in to see 8F-12 9
1 ABIN255816 FACS IHC (fro) IHC ELISA WB Rat IgG1 Log in to see MP4-25D2 0
1 ABIN225255 FACS IP IHC ELISA Mouse IgG1 Log in to see 8D4-8 0

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Human
(407), (230), (56), (33), (25), (22), (21), (16), (15), (14), (13), (12), (9), (8), (6), (6), (6), (5), (3), (2), (1)
Host Rat
(296), (243), (219), (10), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is conjugated to PE
(92), (55), (41), (36), (23), (13), (13), (10), (9), (9), (8), (7), (7), (7), (7), (7), (7), (5), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(426), (390), (281), (176), (58), (55), (45), (39), (38), (38), (25), (22), (22), (13), (8), (8), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN2663795) Images
Cross-Reactivity Pig (Porcine), Rhesus Monkey
Purification The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Clone MP4-25D2
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 antibody (ABIN2663795) Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Background IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 antibody (ABIN2663795) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 antibody (ABIN2663795) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-IL-4 antibody (ABIN2663795)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Iwamoto, Iwai, Tsujiyama, Kurahashi, Takeshita, Naoe, Masunaga, Ogawa, Oguchi, Miyazaki: "TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1449-57, 2007

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Andersson, Abrams, Björk, Funa, Litton, Agren, Andersson: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995

Ramanathan, Ingram, Sullivan, Greenberg, Reim, Trotta, Le: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." in: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, 1993

Boulay, Paul: "The interleukin-4 family of lymphokines." in: Current opinion in immunology, Vol. 4, Issue 3, pp. 294-8, 1992

Dullens, De Wit: "Cancer treatment with interleukins 1, 4 and 6 and combinations of cytokines: a review." in: In vivo (Athens, Greece), Vol. 5, Issue 6, pp. 567-70, 1992

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Mahanty, Abrams, King, Limaye, Nutman: "Parallel regulation of IL-4 and IL-5 in human helminth infections." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3567-71, 1992

Paul: "Interleukin-4: a prototypic immunoregulatory lymphokine." in: Blood, Vol. 77, Issue 9, pp. 1859-70, 1991

Chrétien, Van Kimmenade, Pearce, Banchereau, Abrams: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." in: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, 1989

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN2663795) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Interleukin 4 (IL4) antibody (PE) (ABIN2663795) anti-Interleukin 4 (IL4) antibody (PE)