anti-Human IL-4 antibody for Immunohistochemistry (Frozen Sections)

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Epitope
AA 66-120
34
25
10
10
9
7
7
6
6
6
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
338
228
54
26
25
22
21
16
15
13
12
9
8
6
6
6
4
3
2
1
Host
Rabbit
295
204
181
10
6
4
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
90
51
38
32
20
8
8
8
7
6
4
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
374
364
241
159
57
53
38
29
23
23
22
22
22
13
8
8
5
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Options
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Available images

Catalog No. ABIN1859431
$ 340.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16 ABIN3201264 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see 0
5.5 ABIN2475019 IHC (fro) FACS ELISA WB Rat IgG1 Log in to see MP4-25D2 4
4 ABIN119373 EIA FACS IHC (fro) WB Rat IgG1 Log in to see MP4-25D2 6
4 ABIN217644 ELISA FACS IHC IHC (fro) WB Rat IgG1 Log in to see 0
1 ABIN601663 ELISA FACS IHC IHC (fro) WB Rat IgG1 Log in to see 0
1 ABIN115763 EIA Func IHC (fro) Mouse IgG1 Log in to see 1
1 ABIN640525 IHC (fro) FACS ELISA WB Rat IgG1 Log in to see 0

General

Antigen Interleukin 4 (IL4) Antibodies
Epitope AA 66-120
(34), (25), (10), (10), (9), (7), (7), (6), (6), (6), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(338), (228), (54), (26), (25), (22), (21), (16), (15), (13), (12), (9), (8), (6), (6), (6), (4), (3), (2), (1)
Host Rabbit
(295), (204), (181), (10), (6), (4)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(90), (51), (38), (32), (20), (8), (8), (8), (7), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(374), (364), (241), (159), (57), (53), (38), (29), (23), (23), (22), (22), (22), (13), (8), (8), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Sequence MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- KETFC RAATVLRQFY SHHEKDTRCL GATAQQFHRH KQLIRFLKRL DRNLWGLAGL
Specificity It has been selected for its ability to recognize IL4 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen IL4 (AA 66-120)
Isotype IgG
Plasmids, Primers & others

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant IL4 (Lys66~Leu120) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 4 (IL4) (AA 66-120) antibody (ABIN1859431) anti-Interleukin 4 (IL4) (AA 66-120) antibody
Immunohistochemistry (IHC) image for anti-Interleukin 4 (IL4) (AA 66-120) antibody (ABIN1859431) Figure. DAB staining on IHC-P; Samples: Human Glioma Tissue.
Immunohistochemistry (IHC) image for anti-Interleukin 4 (IL4) (AA 66-120) antibody (ABIN1859431) Figure. DAB staining on IHC-P; Samples: Human Kidney Tissue.
Immunohistochemistry (IHC) image for anti-Interleukin 4 (IL4) (AA 66-120) antibody (ABIN1859431) Figure. DAB staining on IHC-P; Samples: Human Stomach Tissue.
Immunohistochemistry (IHC) image for anti-Interleukin 4 (IL4) (AA 66-120) antibody (ABIN1859431) Figure. DAB staining on IHC-P; Samples: Human Liver Tissue.