anti-Human IL-4 antibody for Neutralization

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Reactivity
Human
396
204
52
34
24
20
19
15
14
11
11
9
9
6
6
6
6
5
2
1
Host
Rabbit
272
229
200
7
6
4
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
86
52
33
30
22
13
13
9
9
8
8
7
7
7
7
7
5
5
4
4
4
3
2
2
2
2
1
1
Application
Immunohistochemistry (IHC), Neutralization (Neut), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
409
393
258
127
56
45
39
38
32
23
22
22
15
10
9
8
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN964756
$ 389.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1102500 IHC (p) Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1177267 ICS Neut ELISA Rat IgG1 Log in to see MP4-25D2 4
1 ABIN2451297 IP IHC (p) Neut ELISA WB Rabbit IgG AA 25-153 Log in to see Polyclonal 0
1 ABIN1889568 Neut IHC WB Mouse IgG1 AA 25-153 Log in to see MM0404-4Z33 0
1 ABIN2290325 IP Neut IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN962202 Neut Rat IgG1, kappa Log in to see MP4-25D2 0
1 ABIN346565 Neut ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN2290130 Neut Rabbit IgG Log in to see 13G43 0
1 ABIN4325241 BP IHC IHC (fro) Neut WB Mouse IgG1 Log in to see MM0404-4Z33 0
1 ABIN2568260 Func Neut Rat IgG1 kappa Log in to see MP4-25D2 0
1 ABIN2568261 Func Neut Rat IgG1 kappa Log in to see MP4-25D2 0
1 ABIN2289255 FACS IF Neut ELISA WB Rat IgG1 kappa Log in to see 5K302 0
1 ABIN4325267 BP ICC IF Neut WB Mouse IgG1 Log in to see 4D9 0
1 ABIN2289213 Neut IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2289109 Neut IHC ELISA WB APC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2289113 Neut IHC ELISA WB FITC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2289117 Neut IHC ELISA WB PE Rabbit IgG Log in to see Polyclonal 0
1 ABIN2289112 Neut IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN2289115 Neut IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN2289216 Neut IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal 0

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Human
(396), (204), (52), (34), (24), (20), (19), (15), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (1)
Host Rabbit
(272), (229), (200), (7), (6), (4)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(86), (52), (33), (30), (22), (13), (13), (9), (9), (8), (8), (7), (7), (7), (7), (7), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Immunohistochemistry (IHC), Neutralization (Neut), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(409), (393), (258), (127), (56), (45), (39), (38), (32), (23), (22), (22), (15), (10), (9), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Specificity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-4 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics IL-4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. Anti-IL-4 antibody is ideal for investigators involved in Cancer and Immunology research.
Purification purified
Immunogen This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-4 protein.
Immunogen Type: RecombinantProtein
Isotype IgG

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Background IL-4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. Anti-IL-4 antibody is ideal for investigators involved in Cancer and Immunology research.
Synonyms: BCGF-1, BCGF1, Binetrakin, BSF 1, BSF1, HCGF, IA Inducing Factor, IGG1 induction factor, IL 4, Il4e12, Interleukin 4, Interleukin 4, isoform 1, Interleukin4, Lymphocyte stimulatory factor 1, MCGF2, MFF, Pitrakinra, TCGF2
Gene ID 3565
NCBI Accession NP_000580
UniProt P05112
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 15 kDa in size corresponding to native human IL-4 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Comment

Gene Name: IL4

Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 4 (IL4) antibody (ABIN964756) anti-Human IL-4 antibody shows detection of a band ~15 kDa in size corresponding to r...
Western Blotting (WB) image for anti-Interleukin 4 (IL4) antibody (ABIN964756) anti-Human IL-4 antibody shows detection of a band ~15 kDa in size corresponding to r...