anti-Mouse (Murine) IL-4 antibody for Flow Cytometry

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Reactivity
Mouse (Murine)
397
203
52
34
24
20
19
15
14
11
11
9
9
6
6
6
6
5
2
1
Host
Rat
273
229
199
7
6
4
Clonality
Monoclonal
Conjugate
This IL-4 antibody is conjugated to PE/Dazzle™ 594
86
52
33
30
22
13
13
9
9
8
8
7
7
7
7
7
5
5
4
4
3
3
2
2
2
2
1
1
Application
Flow Cytometry (FACS)
410
394
257
128
57
45
39
38
32
23
22
22
15
10
9
8
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2659779
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.93816 ABIN2663790 FACS PE Rat IgG1 kappa Log in to see 11B11 9
13.93816 ABIN2660209 FACS PerCP-Cy5.5 Rat IgG1 kappa Log in to see 11B11 9
13.93816 ABIN2659373 FACS PE-Cy7 Rat IgG1 kappa Log in to see 11B11 9
13.93816 ABIN2657548 FACS Alexa Fluor 488 Rat IgG1 kappa Log in to see 11B11 9
13.93816 ABIN2657962 FACS Alexa Fluor 647 Rat IgG1 kappa Log in to see 11B11 9
13.93816 ABIN2658785 FACS APC Rat IgG1 kappa Log in to see 11B11 9
13.93816 ABIN651434 FACS IHC (p) WB Rabbit Ig Fraction AA 122-150, C-Term Log in to see Polyclonal 1
13.93816 ABIN3031478 FACS IHC ELISA WB Rabbit Ig Fraction AA 122-150 Log in to see Polyclonal 0
1 ABIN118404 ELISA (Capture) EIA FACS IHC (fro) WB Rat IgG1 Log in to see BVD4-1D11 3
1 ABIN2666014 FACS Rat IgG1 kappa Log in to see 11B11 9
1 ABIN2661233 FACS Biotin Rat IgG1, IgG1 kappa Log in to see BVD6-24G2 9
1 ABIN255815 FACS IHC (fro) IHC ELISA WB Rat IgG1 Log in to see BVD4-1D11 0
1 ABIN217614 FACS IHC (fro) ELISA WB Rat IgG1 Log in to see 0
1 ABIN2665171 FACS Rat IgG1 kappa Log in to see 11B11 9
1 ABIN5531473 FACS IHC (p) WB Rabbit Ig Fraction AA 122-150, C-Term Log in to see Polyclonal 0
1 ABIN2659780 FACS PE/Dazzle™ 594 Rat IgG1 kappa Log in to see 11B11 11
1 ABIN2658786 FACS APC Rat IgG1 kappa Log in to see 11B11 10
1 ABIN1177017 FACS Neut ELISA WB Rat IgG1 Log in to see 11B11 5
1 ABIN181862 EIA FACS IHC (fro) Rat IgG1 Log in to see BVD6-24G2 4
1 ABIN2665938 FACS Rat IgG1 kappa Log in to see 11B11 9

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Mouse (Murine)
(397), (203), (52), (34), (24), (20), (19), (15), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (1)
Host Rat
(273), (229), (199), (7), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is conjugated to PE/Dazzle™ 594
(86), (52), (33), (30), (22), (13), (13), (9), (9), (8), (8), (7), (7), (7), (7), (7), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS)
(410), (394), (257), (128), (57), (45), (39), (38), (32), (23), (22), (22), (15), (10), (9), (8), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN2659779) Images
Purification The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.
Clone 11B11
Isotype IgG1 kappa

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 antibody (ABIN2659779) Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Background IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development.
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 antibody (ABIN2659779) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 antibody (ABIN2659779) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-IL-4 antibody (ABIN2659779)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Fan, Kouda, Nakamura, Takeuchi: "Effects of dietary restriction on spontaneous dermatitis in NC/Nga mice." in: Experimental biology and medicine (Maywood, N.J.), Vol. 226, Issue 11, pp. 1045-50, 2001

Hara, Kingsley, Niimi, Read, Turvey, Bushell, Morris, Powrie, Wood: "IL-10 is required for regulatory T cells to mediate tolerance to alloantigens in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 3789-96, 2001

Openshaw, Murphy, Hosken, Maino, Davis, Murphy, OGarra: "Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1357-67, 1995

Shirai, Sierra, Kelly, Klinman: "Individual cells simultaneously produce both IL-4 and IL-6 in vivo." in: Cytokine, Vol. 6, Issue 3, pp. 329-36, 1994

Assenmacher, Schmitz, Radbruch: "Flow cytometric determination of cytokines in activated murine T helper lymphocytes: expression of interleukin-10 in interferon-gamma and in interleukin-4-expressing cells." in: European journal of immunology, Vol. 24, Issue 5, pp. 1097-101, 1994

Litton, Sander, Murphy, OGarra, Abrams: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, 1994

Boulay, Paul: "The interleukin-4 family of lymphokines." in: Current opinion in immunology, Vol. 4, Issue 3, pp. 294-8, 1992

Dullens, De Wit: "Cancer treatment with interleukins 1, 4 and 6 and combinations of cytokines: a review." in: In vivo (Athens, Greece), Vol. 5, Issue 6, pp. 567-70, 1992

Paul: "Interleukin-4: a prototypic immunoregulatory lymphokine." in: Blood, Vol. 77, Issue 9, pp. 1859-70, 1991

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN2659779) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Interleukin 4 (IL4) antibody (PE/Dazzle™ 594) (ABIN2659779) anti-Interleukin 4 (IL4) antibody (PE/Dazzle™ 594)
Flow Cytometry (FACS) image for anti-Interleukin 4 (IL4) antibody (PE/Dazzle™ 594) (ABIN2659779) anti-Interleukin 4 (IL4) antibody (PE/Dazzle™ 594) (Image 2)