anti-Mouse (Murine) IL-4 antibody for Functional Studies

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Reactivity
Mouse (Murine)
408
229
56
33
25
22
21
16
15
14
13
12
9
8
6
6
6
5
3
2
1
Host
Rat
296
243
219
10
6
4
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
92
56
41
36
23
13
13
10
9
9
8
7
7
7
7
7
7
5
5
4
4
3
2
2
2
2
1
1
1
1
1
1
Application
Functional Studies (Func), Western Blotting (WB)
425
397
282
176
59
55
45
39
38
38
25
22
21
13
8
8
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN5610815
$ 1,242.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115882 EIA Func WB Rabbit Log in to see Polyclonal 2
1 ABIN115881 EIA Func WB Rabbit Log in to see Polyclonal 2
1 ABIN208728 Func Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2475015 Func ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN2568255 Func FACS IF IHC ELISA Rat IgG1 kappa Log in to see 11B11 0
1 ABIN2568254 Func FACS IF IHC ELISA Rat IgG1 kappa Log in to see 11B11 0
1 ABIN2144133 Func FACS Mouse IgG1 kappa Log in to see 8D4-8 0
1 ABIN641027 Func ELISA WB Rabbit Log in to see 0

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Mouse (Murine)
(408), (229), (56), (33), (25), (22), (21), (16), (15), (14), (13), (12), (9), (8), (6), (6), (6), (5), (3), (2), (1)
Host Rat
(296), (243), (219), (10), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(92), (56), (41), (36), (23), (13), (13), (10), (9), (9), (8), (7), (7), (7), (7), (7), (7), (5), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Western Blotting (WB)
(425), (397), (282), (176), (59), (55), (45), (39), (38), (38), (25), (22), (21), (13), (8), (8), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Specificity This antibody is specific to IL-4.
Characteristics Synonyms: IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra, B-cell stimulatory factor1
Purification S-Sepharose and Protein A chromatography
Immunogen Recombinant mouse IL-4
Clone I4-F5
Isotype IgG1
Plasmids, Primers & others

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-4 / IL4 (IL4 Antibody Abstract)
Background IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 16189
UniProt P07750
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes 1: 50 dilution will block 80-85 % of CTLL or HT-2 proliferative response to 20 ng/mLrecombinant murine IL-4.1: 3,000 in Western blot using recombinant IL-4.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Reconstitution Restore with 0.2 mL water. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Concentration 1,0 mg/mL (prior to lyophilization)
Buffer PBS
Preservative Azide free
Storage -20 °C
Storage Comment Prior to and following reconstitution store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months