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|Antigen||Interleukin 4 (IL4) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This IL-4 antibody is un-conjugated Alternatives|
Functional Studies (Func), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-IL-4 AntibodyTarget Details IL-4 Application Details Handling Images
|Specificity||This antibody is specific to IL-4.|
|Characteristics||Synonyms: IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra, B-cell stimulatory factor1|
|Purification||S-Sepharose and Protein A chromatography|
|Immunogen||Recombinant mouse IL-4|
Target Details IL-4Product Details anti-IL-4 Antibody Application Details Handling Images back to top
|Alternative Name||Interleukin-4 / IL4 (IL4 Antibody Abstract)|
|Background||IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra|
|Pathways||JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation|
Application DetailsProduct Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
1: 50 dilution will block 80-85 % of CTLL or HT-2 proliferative response to 20 ng/mLrecombinant murine IL-4.1: 3,000 in Western blot using recombinant IL-4.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
|Reconstitution||Restore with 0.2 mL water. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.|
|Concentration||1,0 mg/mL (prior to lyophilization)|
Prior to and following reconstitution store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|