anti-Mouse (Murine) IL-4 antibody for Immunofluorescence

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Reactivity
Mouse (Murine)
393
199
51
32
24
20
19
15
14
11
11
9
9
6
6
6
6
5
2
1
Host
Rat
271
226
194
7
6
4
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
85
52
33
30
22
13
13
9
9
8
7
7
7
7
7
7
5
5
4
4
4
3
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Western Blotting (WB)
401
387
250
128
55
45
39
37
32
23
22
22
15
10
9
8
5
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN218112
$ 713.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN218113 FACS IF Neut ELISA WB Rat IgG2b, kappa Log in to see 0
1 ABIN957628 FACS IF ELISA WB Rat IgG2b, kappa Log in to see BVD4-1D11 0
1 ABIN2568255 Func FACS IF IHC ELISA Rat IgG1 kappa Log in to see 11B11 0
1 ABIN2568254 Func FACS IF IHC ELISA Rat IgG1 kappa Log in to see 11B11 0
1 ABIN2289227 FACS IF ELISA WB Rat IgG2b kappa Log in to see 5K310 0
1 ABIN2289229 FACS IF Neut ELISA WB Rat IgG2b kappa Log in to see 5K310 0

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Mouse (Murine)
(393), (199), (51), (32), (24), (20), (19), (15), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (1)
Host Rat
(271), (226), (194), (7), (6), (4)
Clonality
Monoclonal
Conjugate This IL-4 antibody is un-conjugated
(85), (52), (33), (30), (22), (13), (13), (9), (9), (8), (7), (7), (7), (7), (7), (7), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Western Blotting (WB)
(401), (387), (250), (128), (55), (45), (39), (37), (32), (23), (22), (22), (15), (10), (9), (8), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Specificity Recognizes mouse Interleukin-4 (IL-4).
Purification Purified
Immunogen E. coli-expressed recombinant mouse IL-4.
Isotype IgG2b, kappa

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL4 (IL4 Antibody Abstract)
Background Name/Gene ID: IL4
Family: Hormone

Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4
Gene ID 3565
UniProt P05112
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Approved: ELISA, Flo, IF, WB

Usage: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-4 protein levels.1-4 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b should be used as a specificity control. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-4 under reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Preservative Without preservative
Storage 4 °C,-20 °C
Storage Comment Long term: Add glycerol (40-50%) -20°C
Short term: +4°C