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|Antigen||Interleukin 4 (IL4) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This IL-4 antibody is un-conjugated Alternatives|
ELISA (Capture), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|3 references available|
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Product Details anti-IL-4 AntibodyTarget Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN118404) Images
|Specificity||This antibody is specific for Mouse Interleukin-4 (IL-4). Clone BVD4-1D11 is reported to neutralise the effects of IL-4. We recommend the use of SM101A for functional studies.|
|No Cross-Reactivity||Rat (Rattus)|
|Cross-Reactivity (Details)||Species reactivity (tested):Mouse.|
|Purification||Affinity Chromatography on protein G|
|Immunogen||Recombinant Mouse IL-4. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.|
Target Details IL-4Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 antibody (ABIN118404) Images back to top
|Alternative Name||Interleukin-4 / IL4 (IL4 Antibody Abstract)|
|Background||Interleukin-4 is a potent lymphoid growth factor, which stimulates the growth and survival of certain B and T cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra|
|Pathways||JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation|
Application DetailsProduct Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 antibody (ABIN118404) Images back to top
Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required. Immunohistochemistry on frozen sections. ELISA: 1 μg/mL - 5 μg/mL. Western Blot: 1 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 antibody (ABIN118404) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-IL-4 antibody (ABIN118404)Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Hochrein, OKeeffe, Luft, Vandenabeele, Grumont, Maraskovsky, Shortman: "Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells." in: The Journal of experimental medicine, Vol. 192, Issue 6, pp. 823-33, 2000
Sander, Höidén, Andersson, Möller, Abrams: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994
Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992
ImagesProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN118404) back to top