anti-Mouse (Murine) IL-4 antibody for Neutralization

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Reactivity
Mouse (Murine)
398
202
52
34
24
20
19
15
14
11
11
9
9
6
6
6
6
5
2
1
Host
Rat
273
230
199
7
6
4
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
86
52
33
30
22
13
13
9
9
8
8
7
7
7
7
7
5
5
4
4
4
3
2
2
2
2
1
1
Application
Neutralization (Neut), ELISA, Western Blotting (WB)
410
389
252
128
56
45
39
38
32
23
22
22
15
10
9
8
5
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2689762
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177017 FACS Neut ELISA WB Rat IgG1 Log in to see 11B11 5
1 ABIN1177294 Neut ELISA Rat IgG2b Log in to see BVD4-1D11 4
1 ABIN465884 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN465839 Neut ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN218110 Neut ELISA Rat IgG2b Log in to see 0
1 ABIN2689763 Neut ELISA WB Rat IgG2b Log in to see BVD4 1
1 ABIN218111 Neut ELISA Rat IgG2b Log in to see 0
1 ABIN208728 Func Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN218113 FACS IF Neut ELISA WB Rat IgG2b, kappa Log in to see 0
1 ABIN346573 Neut ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN2289250 Neut ELISA Rat IgG2b Log in to see 5J334 0
1 ABIN2289225 Neut ELISA Rat IgG2b Log in to see 5J334 0
1 ABIN2289229 FACS IF Neut ELISA WB Rat IgG2b kappa Log in to see 5K310 0
1 ABIN2289149 Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2289208 Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1983778 Neut WB Rat IgG1 AA 23-140 Log in to see RM0274-2L55 0
1 ABIN2289098 Neut ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal 0
1 ABIN2289100 Neut ELISA WB APC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2289104 Neut ELISA WB FITC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2289102 Neut ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Mouse (Murine)
(398), (202), (52), (34), (24), (20), (19), (15), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (1)
Host Rat
(273), (230), (199), (7), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(86), (52), (33), (30), (22), (13), (13), (9), (9), (8), (8), (7), (7), (7), (7), (7), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Neutralization (Neut), ELISA, Western Blotting (WB)
(410), (389), (252), (128), (56), (45), (39), (38), (32), (23), (22), (22), (15), (10), (9), (8), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN2689762) Images
Brand BD Pharmingen™
Characteristics The BVD4-1D11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD4-1D11 hybridoma was recombinant mouse IL-4. This is a neutralizing antibody. This antibody is routinely tested by intracellular staining and sandwich ELISA. Other applications were tested during antibody development only or reported in the literature. Expression of IL-4 by MiCK-2 positive control cells. MiCK-2 positive control cells (Cat. No. 554653) were stained with 0.125 μg of FITC-conjugated rat anti-mouse IL-4 antibody by using the BD Pharmingen staining protocol (see left panel). To demonstrate specificity of staining, the binding of FITC-BVD4-1D11 was blocked by the preincubation of the conjugated antibody with recombinant mouse IL-4 (0.25 μg, Cat. No. 550067, see middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled BVD4-1D11 antibody (5 μg, Cat. No. 554386, see right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.

BD Pharmingen™ Purified Rat Anti-Mouse IL-4 - Purified - Clone BVD4-1D11 - Isotype Rat IgG2b - Reactivity Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Recombinant Mouse IL-4
Clone BVD4
Isotype IgG2b

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 antibody (ABIN2689762) Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 antibody (ABIN2689762) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 antibody (ABIN2689762) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-IL-4 antibody (ABIN2689762)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Product cited in:

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Litton, Sander, Murphy, OGarra, Abrams: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, 1994

Finkelman, Madden, Morris, Holmes, Boiani, Katona, Maliszewski: "Anti-cytokine antibodies as carrier proteins. Prolongation of in vivo effects of exogenous cytokines by injection of cytokine-anti-cytokine antibody complexes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1235-44, 1993

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992