anti-Mouse (Murine) IL-4 antibody for Western Blotting

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Epitope
AA 122-150
34
25
10
10
8
7
7
6
6
6
5
4
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
343
231
54
26
25
22
21
16
15
13
12
9
8
6
6
6
4
3
2
1
Host
Rabbit
294
211
179
10
7
4
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
82
52
38
34
20
8
8
8
7
6
4
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
369
360
247
160
55
55
35
30
24
24
23
21
21
11
8
8
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Options
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Available images

Catalog No. ABIN3031478
$ 452.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.212444 ABIN651434 FACS IHC (p) WB Rabbit Ig Fraction AA 122-150, C-Term Log in to see Polyclonal 2
12.073499 ABIN289356 Inhibition ELISA WB Rabbit Log in to see Polyclonal 0
12.073499 ABIN2144136 IP IHC ELISA WB Rat IgG1 Log in to see NYRmIL-4 0
12.073499 ABIN2748988 ELISpot ICS Neut ELISA WB Rat IgG1 Log in to see 11B11 0
10.712444 ABIN6262627 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10.712444 ABIN3002277 FACS IHC WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN5531473 FACS IHC (p) WB Rabbit Ig Fraction AA 122-150, C-Term Log in to see Polyclonal 0
9.212444 ABIN3043168 WB Rabbit IgG AA 123-140, C-Term Log in to see Polyclonal 4
7.7124443 ABIN5013801 ICC IHC WB Rabbit IgG AA 21-140 Log in to see Polyclonal 0
7.7124443 ABIN6000114 IF/ICC IHC IP WB Rabbit AA 21-140 Log in to see Polyclonal 0
7.7124443 ABIN3031479 WB Rabbit IgG C-Term Log in to see Polyclonal 0
7 ABIN465884 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal 0
7 ABIN465839 Neut ELISA WB Rabbit Log in to see Polyclonal 0
5.5 ABIN1177017 FACS Neut ELISA WB Rat IgG1 Log in to see 11B11 5
5.5 ABIN2689762 Neut ELISA WB Rat IgG2b Log in to see BVD4 4
5.5 ABIN2475022 IHC (fro) FACS ELISA WB Rat IgG2b Log in to see BVD4-1D11 4
5.5 ABIN2689763 Neut ELISA WB Rat IgG2b Log in to see BVD4 1
5.5 ABIN2475015 Func ELISA WB Rabbit IgG Log in to see Polyclonal 1
4.7124443 ABIN2704922 WB Rabbit C-Term Log in to see Polyclonal 0
4.7124443 ABIN118404 ELISA (Capture) EIA FACS IHC (fro) WB Rat IgG1 Log in to see BVD4-1D11 3

General

Antigen Interleukin 4 (IL4) Antibodies
Epitope AA 122-150
(34), (25), (10), (10), (8), (7), (7), (6), (6), (6), (5), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(343), (231), (54), (26), (25), (22), (21), (16), (15), (13), (12), (9), (8), (6), (6), (6), (4), (3), (2), (1)
Host Rabbit
(294), (211), (179), (10), (7), (4)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(82), (52), (38), (34), (20), (8), (8), (8), (7), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(369), (360), (247), (160), (55), (55), (35), (30), (24), (24), (23), (21), (21), (11), (8), (8), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Cross-Reactivity (Details) Expected species reactivity: Primate
Purification Antigen affinity purified
Immunogen A portion of amino acids 122-150 from the human protein was used as the immunogen for this IL-4 antibody.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Background IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.
UniProt P05112
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Titration of the IL-4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Liquid
Buffer In 1X PBS pH 7.4 with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot the IL-4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) (Left) Western blot analysis of IL-4 antibody and WiDr lysate; (Right) Western blot a...
Flow Cytometry (FACS) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) IL-4 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a ...
Immunohistochemistry (IHC) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) IL-4 antibody IHC analysis in formalin fixed and paraffin embedded tonsil
Western Blotting (WB) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) (Left) Western blot analysis of IL-4 antibody and WiDr lysate
Flow Cytometry (FACS) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) IL-4 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a ...