anti-Mouse (Murine) IL-4 antibody for Western Blotting

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
Epitope
AA 123-140, C-Term
30
22
13
10
10
8
7
7
6
6
4
4
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
408
229
56
33
25
22
21
16
15
14
13
12
9
8
6
6
6
5
3
2
1
Host
Rabbit
295
243
220
10
6
4
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
92
56
41
36
23
13
13
10
9
9
8
7
7
7
7
7
7
5
5
4
4
3
2
2
2
2
1
1
1
1
1
1
Application
Western Blotting (WB)
425
390
282
176
58
55
45
39
38
38
25
22
22
13
8
8
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN3043168
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1680813 ABIN2704922 WB Rabbit C-Term Log in to see Polyclonal 0
3.1680813 ABIN118404 ELISA (Capture) EIA FACS IHC (fro) WB Rat IgG1 Log in to see BVD4-1D11 3
3.1680813 ABIN651434 FACS IHC (p) WB Rabbit Ig Fraction AA 122-150, C-Term Log in to see Polyclonal 1
3.1680813 ABIN255815 FACS IHC (fro) IHC ELISA WB Rat IgG1 Log in to see BVD4-1D11 0
3.1680813 ABIN439189 IHC (p) WB Rabbit Log in to see Polyclonal 0
3.1680813 ABIN1862772 ICC IHC WB Rabbit IgG AA 23-140 Log in to see Polyclonal 0
3.1680813 ABIN3031478 FACS IHC ELISA WB Rabbit Ig Fraction AA 122-150 Log in to see Polyclonal 0
3.1680813 ABIN5013801 ICC IHC WB Rabbit IgG AA 21-140 Log in to see Polyclonal 0
3.1680813 ABIN3031479 WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2689762 Neut ELISA WB Rat IgG2b Log in to see BVD4 4
1 ABIN217614 ELISA FACS IHC IHC (fro) WB Rat IgG1 Log in to see 0
1 ABIN5531473 FACS IHC (p) WB Rabbit Ig Fraction AA 122-150, C-Term Log in to see Polyclonal 0
1 ABIN465884 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN1584604 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN465839 Neut ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN1177017 FACS Neut ELISA WB Rat IgG1 Log in to see 11B11 5
1 ABIN2624132 WB Rabbit IgG AA 123-140 Log in to see Polyclonal 0
1 ABIN2624138 ELISA WB Biotin Rat IgG1 Log in to see 0
1 ABIN1825563 IP IHC ELISA WB Rat IgG1 Log in to see NYRmIL-4 0
1 ABIN218103 ELISA FACS IHC IHC (fro) WB Rat IgG1 Log in to see 0

General

Antigen Interleukin 4 (IL4) Antibodies
Epitope AA 123-140, C-Term
(30), (22), (13), (10), (10), (8), (7), (7), (6), (6), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(408), (229), (56), (33), (25), (22), (21), (16), (15), (14), (13), (12), (9), (8), (6), (6), (6), (5), (3), (2), (1)
Host Rabbit
(295), (243), (220), (10), (6), (4)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(92), (56), (41), (36), (23), (13), (13), (10), (9), (9), (8), (7), (7), (7), (7), (7), (7), (5), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(425), (390), (282), (176), (58), (55), (45), (39), (38), (38), (25), (22), (22), (13), (8), (8), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN3043168) Images
Purpose Rabbit IgG polyclonal antibody for Interleukin-4(IL4) detection. Tested with WB in Mouse.
Sequence SLKDFLESLK SIMQMDYS
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Interleukin-4(IL4) detection. Tested with WB in Mouse.
Gene Name: interleukin 4
Protein Name: Interleukin-4(IL-4)
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL-4(123-140aa SLKDFLESLKSIMQMDYS).
Isotype IgG
Plasmids, Primers & others

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 antibody (ABIN3043168) Images back to top
Antigen
Alternative Name IL4 (IL4 Antibody Abstract)
Background Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo, in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2 The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Synonyms: B cell growth factor 1 antibody|B cell IgG differentiation factor antibody|B Cell Stimulatory Factor 1 antibody|B-cell stimulatory factor 1 antibody|BCGF 1 antibody|BCGF1 antibody|Binetrakin antibody|BSF 1 antibody|BSF-1 antibody|BSF1 antibody|HCGF antibody|Hodgkin's Cell Growth Factor antibody|IA Inducing Factor antibody|IGG1 induction factor antibody|IL 4 antibody|IL-4 antibody|IL4 antibody|IL4_HUMAN antibody|Il4e12 antibody|Interleukin 4 antibody|Interleukin 4, isoform 1 antibody|Interleukin-4 antibody|Interleukin4 antibody|Lymphocyte stimulatory factor 1 antibody|Macrophage fusion factor antibody|Mast cell growth factor 2 antibody|MCGF2 antibody|MFF antibody|MGC79402 antibody|Pitrakinra antibody|T cell growth factor 2 antibody|TCGF2 antibody
UniProt P07750
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 antibody (ABIN3043168) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 antibody (ABIN3043168) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-IL-4 antibody (ABIN3043168)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Product cited in:

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, 2015

Yan, Wang, Liang, Fu, Guo: "HPV16L1-attenuated Shigella recombinant vaccine induced strong vaginal and systemic immune responses in guinea pig model." in: Human vaccines & immunotherapeutics, Vol. 10, Issue 12, pp. 3491-8, 2015

Wang, Feng, Shi, Hao, Yang, Zhang, Kong: "Silencing of miR155 promotes the production of inflammatory mediators in Guillain-Barré syndrome in vitro." in: Inflammation, Vol. 36, Issue 2, pp. 337-45, 2013

Tan, Liu, Yang, Wang, Qin: "An inactivated Pseudomonas aeruginosa medicament inhibits airway allergic inflammation and improves epithelial functions." in: The journal of physiological sciences : JPS, Vol. 63, Issue 1, pp. 63-9, 2012

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 antibody (ABIN3043168) back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 4 (IL4) (AA 123-140), (C-Term) antibody (ABIN3043168) Anti-IL4 antibody, Western blotting Lane 1: Recombinant Mouse IL-4 Protein 10ng Lane ...