anti-Human IL12 antibody for Western Blotting

Recommended IL12 Antibody (supplied by: Log in to see )

Antigen
Interleukin 12 (IL12) Antibodies
  • CLMF
  • CLMF2
  • IL-12B
  • NKSF
  • NKSF2
  • p40
  • Il-12b
  • Il12p40
  • Il-12p40
  • Il12
  • IL-12p40
  • IL-12
  • LOC100217488
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • IL12p40
  • IL12B
  • IL-12 p35
  • interleukin 12B
  • interleukin 12A
  • interleukin 12b
  • interleukin 12a
  • Interleukin 12 level
  • IL12B
  • IL12A
  • Il12b
  • Il12a
  • IL12
  • il12a
Epitope
AA 23-219
17
15
13
5
4
2
2
1
1
1
1
1
1
1
Reactivity
Human
181
75
18
9
6
4
3
3
2
2
2
1
1
1
Host
Mouse
139
52
43
43
2
1
Clonality
Monoclonal
Conjugate
This IL12 antibody is un-conjugated
37
15
10
9
8
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
186
160
109
40
30
27
25
19
19
13
11
6
6
5
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN3201242
$ 235.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.8412423 ABIN652878 FACS IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal 1
2.8412423 ABIN1172115 IHC ELISA WB Biotin Rabbit IgG AA 23-219 Log in to see Polyclonal 0
2.8412423 ABIN1172113 ICC IHC WB Rabbit IgG AA 23-219 Log in to see Polyclonal 0
2.8412423 ABIN3031445 FACS IHC ELISA WB Rabbit Ig Fraction AA 168-195 Log in to see Polyclonal 0
2.8412423 ABIN5662287 IHC WB Mouse AA 23-219 Log in to see 0
2.8412423 ABIN5662288 IHC WB Mouse AA 23-219 Log in to see 0
2.8412423 ABIN2935354 IF/ICC IHC IP WB Rabbit AA 23-219 Log in to see Polyclonal 0
2.8412423 ABIN6002591 IF/ICC IHC IP WB Mouse AA 23-219 Log in to see 0
2.8412423 ABIN6002589 IF/ICC IHC IP WB Mouse AA 23-219 Log in to see 0
2.8412423 ABIN6002587 IF/ICC IHC IP WB Mouse AA 23-219 Log in to see 0
1 ABIN734453 IF (p) IHC (p) WB Rabbit IgG AA 60-85 Log in to see Polyclonal 1
1 ABIN411514 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN734462 IHC (p) WB HRP Rabbit IgG AA 60-85 Log in to see Polyclonal 0
1 ABIN465143 ELISA WB Mouse IgG1 Log in to see 0
1 ABIN734455 IHC (p) WB Biotin Rabbit IgG AA 60-85 Log in to see Polyclonal 0
1 ABIN465123 ELISA WB Biotin Goat Log in to see Polyclonal 0
1 ABIN465439 Neut ELISA WB Goat Log in to see Polyclonal 0
1 ABIN2289958 WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN4324478 BP ELISA Neut WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4224700 ELISA IHC RIA WB Mouse IgG1 Log in to see 1-1A4 0

General

Antigen Interleukin 12 (IL12) Antibodies
Epitope AA 23-219
(17), (15), (13), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(181), (75), (18), (9), (6), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(139), (52), (43), (43), (2), (1)
Clonality
Monoclonal
Conjugate This IL12 antibody is un-conjugated
(37), (15), (10), (9), (8), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(186), (160), (109), (40), (30), (27), (25), (19), (19), (13), (11), (6), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL12 Antibody

Target Details IL12 Application Details Handling Images
Sequence MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EFELRRQ- RNLPVATP DPGMFPCLHH SQNLLRAVSN MLQKARQTLE FYPCTSEEID HEDITKDKTS TVEACLPLEL TKNESCLNSR ETSFITNGSC LASRKTSFMM ALCLSSIYED LKMYQVEFKT MNAKLLMDPK RQIFLDQNML AVIDELMQAL NFNSETVPQK SSLEEPDFYK TKIKLCILLH AFRIRAVTID RVMSYLNAS
Specificity It has been selected for its ability to recognize IL12A in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen IL12A (AA 23-219)
Isotype IgG
Plasmids, Primers & others

Target Details IL12

Product Details anti-IL12 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin 12A (IL12A) (IL12 Antibody Abstract)
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-IL12 Antibody Target Details IL12 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Restrictions For Research Use only

Handling

Product Details anti-IL12 Antibody Target Details IL12 Application Details Images back to top
Format Liquid
Concentration 500 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-IL12 Antibody Target Details IL12 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 12 (IL12) (AA 23-219) antibody (ABIN3201242) anti-Interleukin 12 (IL12) (AA 23-219) antibody
Immunohistochemistry (IHC) image for anti-Interleukin 12 (IL12) (AA 23-219) antibody (ABIN3201242) Figure. DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.
Immunohistochemistry (IHC) image for anti-Interleukin 12 (IL12) (AA 23-219) antibody (ABIN3201242) Figure. DAB staining on IHC-P; Samples: Human Skin Cancer Tissue.
Immunohistochemistry (IHC) image for anti-Interleukin 12 (IL12) (AA 23-219) antibody (ABIN3201242) Figure. DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.
Immunohistochemistry (IHC) image for anti-Interleukin 12 (IL12) (AA 23-219) antibody (ABIN3201242) Figure. DAB staining on IHC-P; Samples: Human Stomach Tissue.
Western Blotting (WB) image for anti-Interleukin 12 (IL12) (AA 23-219) antibody (ABIN3201242) Western Blot; Sample: Mouse RAW264.7 cell lysate; Primary Ab: 3µg/ml Mouse Anti-Human...