anti-Rat (Rattus) IL12B antibody for Western Blotting

Recommended IL12B Antibody (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) Antibodies
  • CLMF
  • CLMF2
  • IL-12B
  • NKSF
  • NKSF2
  • p40
  • Il-12b
  • Il12p40
  • Il-12p40
  • Il12
  • IL-12p40
  • IL-12
  • IL-12 p40
  • il12b
  • il12p40.b
  • zgc:152789
  • IL-12.p40
  • IL12p40
  • IL12B
  • CLMF p40
  • interleukin 12B
  • interleukin 12b
  • interleukin 12Ba
  • IL12B
  • Il12b
  • il12ba
Epitope
AA 23-250
24
15
13
10
10
9
8
8
7
7
7
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
157
69
29
25
19
5
3
3
2
2
2
1
1
1
1
Host
Rabbit
152
64
35
11
4
1
Clonality
Polyclonal
Conjugate
This IL12B antibody is un-conjugated
21
16
15
7
6
6
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
202
167
85
27
26
21
18
17
6
5
5
3
3
2
1
1
1
Options
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Catalog No. ABIN5519010
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.302607 ABIN2176964 IHC (p) WB Rabbit IgG AA 220-255 Log in to see Polyclonal 1
9.302607 ABIN3042735 WB Rabbit IgG AA 23-321 Log in to see Polyclonal 1
9.302607 ABIN5519009 ELISA WB Rabbit IgG AA 23-250 Log in to see Polyclonal 1
7.8026066 ABIN1868574 ICC IHC WB Rabbit IgG AA 30-311 Log in to see Polyclonal 0
7.8026066 ABIN2935363 IF/ICC IHC IP WB Rabbit AA 30-311 Log in to see Polyclonal 0
4.8026066 ABIN4904029 WB Rabbit IgG Log in to see Polyclonal 0
4.8026066 ABIN2465843 ELISA WB Goat Internal Region Log in to see Polyclonal 0
4.8026066 ABIN5995886 WB Rabbit IgG Log in to see Polyclonal 0
4.8026066 ABIN2563391 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
4.8026066 ABIN3028751 ELISA WB Rabbit Ig Fraction Log in to see Polyclonal 0
4 ABIN296296 ELISA WB Goat AA 211-222 Log in to see Polyclonal 0
1 ABIN5553442 WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN2741943 WB Rabbit IgG AA 23-321 Log in to see Polyclonal 0
1 ABIN2176966 IHC (p) WB HRP Rabbit IgG AA 220-255 Log in to see Polyclonal 0
1 ABIN2176965 IHC (p) WB Biotin Rabbit IgG AA 220-255 Log in to see Polyclonal 0
1 ABIN2750281 ELISA IP WB FITC Rabbit IgG AA 300-350 Log in to see Polyclonal 0
1 ABIN2750280 ELISA IP WB Rabbit IgG AA 300-350 Log in to see Polyclonal 0
1 ABIN2741942 ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN4951447 ELISA WB Goat Ig Fraction Log in to see Polyclonal 0
1 ABIN3031447 WB Rabbit IgG AA 23-321 Log in to see Polyclonal 0

General

Antigen Interleukin 12b (IL12B) Antibodies
Epitope AA 23-250
(24), (15), (13), (10), (10), (9), (8), (8), (7), (7), (7), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(157), (69), (29), (25), (19), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(152), (64), (35), (11), (4), (1)
Clonality
Polyclonal
Conjugate This IL12B antibody is un-conjugated
(21), (16), (15), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(202), (167), (85), (27), (26), (21), (18), (17), (6), (5), (5), (3), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL12B Antibody

Target Details IL12B Application Details Handling References for anti-IL12B antibody (ABIN5519010) Images
Purpose Rabbit IgG polyclonal antibody for IL12B detection. Tested with WB, IHC-P, Direct ELISA in Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for IL12B detection. Tested with WB, IHC-P, Direct ELISA in Mouse,Rat.
Gene Name: interleukin 12B
Protein Name: Interleukin-12 subunit beta
Purification Immunogen affinity purified.
Immunogen E. coli-derived rat IL12B recombinant protein (Position: M23-E250).
Isotype IgG
Plasmids, Primers & others

Target Details IL12B

Product Details anti-IL12B Antibody Application Details Handling References for anti-IL12B antibody (ABIN5519010) Images back to top
Antigen
Alternative Name Il12b (IL12B Antibody Abstract)
Background Subunit beta of interleukin 12 (IL12B), also known as interleukin 12B, or interleukin-12 subunit p40, is a subunit of human interleukin 12. It is mapped to 5q33.3. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease.

Synonyms: Interleukin-12 subunit beta
Gene ID 64546
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-IL12B Antibody Target Details IL12B Handling References for anti-IL12B antibody (ABIN5519010) Images back to top
Application Notes Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-IL12B Antibody Target Details IL12B Application Details References for anti-IL12B antibody (ABIN5519010) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-IL12B antibody (ABIN5519010)

Product Details anti-IL12B Antibody Target Details IL12B Application Details Handling Images back to top
Product cited in:

Dong, Xia, Chen, Chen, Zhang: "Anti-arthritis activity of cationic materials." in: Journal of cellular and molecular medicine, Vol. 14, Issue 7, pp. 2015-24, 2011

Images

Product Details anti-IL12B Antibody Target Details IL12B Application Details Handling References for anti-IL12B antibody (ABIN5519010) back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 12b (IL12B) (AA 23-250) antibody (ABIN5519010) Western blot analysis of IL12B using anti-IL12B antibody . Electrophoresis was perfor...
Immunohistochemistry (IHC) image for anti-Interleukin 12b (IL12B) (AA 23-250) antibody (ABIN5519010) IHC analysis of IL12B using anti-IL12B antibody .IL12B was detected in paraffin-embed...