anti-Human IL2 antibody for Radioimmunoassay

Recommended IL2 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) Antibodies
  • IL2
  • IL-2
  • il2
  • TCGF
  • lymphokine
  • Il-2
  • POIL2
  • interleukin 2
  • interleukin-15
  • IL2
  • il2
  • LOC100303688
  • Il2
Reactivity
Human
481
247
99
29
17
10
6
6
5
4
3
3
1
1
Host
Rabbit
314
281
210
18
5
4
4
Clonality
Polyclonal
Conjugate
This IL2 antibody is un-conjugated
93
50
50
29
28
18
17
14
12
11
9
8
7
7
7
7
7
7
7
6
6
6
4
4
4
4
4
4
4
3
3
2
2
2
1
1
Application
Immunohistochemistry (IHC), Neutralization (Neut), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
468
404
225
92
72
50
49
42
41
40
27
26
15
11
11
7
7
7
5
5
4
3
3
2
2
1
1
1
1
1
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Available images

Catalog No. ABIN964758
$ 389.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN603354 IP Neut RIA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5565656 EIA RIA Biotin Rabbit AA 22-153 Log in to see Polyclonal 0
1 ABIN5565654 EIA RIA Rabbit AA 22-153 Log in to see Polyclonal 0
1 ABIN2288455 IP Neut RIA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2192177 IA Neut RIA WB Rabbit Log in to see Polyclonal 0

General

Antigen Interleukin 2 (IL2) Antibodies
Reactivity Human
(481), (247), (99), (29), (17), (10), (6), (6), (5), (4), (3), (3), (1), (1)
Host Rabbit
(314), (281), (210), (18), (5), (4), (4)
Clonality
Polyclonal
Conjugate This IL2 antibody is un-conjugated
(93), (50), (50), (29), (28), (18), (17), (14), (12), (11), (9), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Application Immunohistochemistry (IHC), Neutralization (Neut), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(468), (404), (225), (92), (72), (50), (49), (42), (41), (40), (27), (26), (15), (11), (11), (7), (7), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-IL2 Antibody

Target Details IL2 Application Details Handling Images
Specificity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-2 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics IL-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. Anti-IL-2 antibody is ideal for investigators involved in Immunology research.
Purification purified
Immunogen IL-2 antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-2 protein.
Immunogen Type: RecombinantProtein
Isotype IgG

Target Details IL2

Product Details anti-IL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-2 (IL2 Antibody Abstract)
Background IL-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. Anti-IL-2 antibody is ideal for investigators involved in Immunology research.
Synonyms: Interleukin-2, IL-2, IL2, T-cell growth factor, TCGF, Aldesleukin, Lymphokine
Gene ID 3558
NCBI Accession NP_000577
UniProt P60568
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation

Application Details

Product Details anti-IL2 Antibody Target Details IL2 Handling Images back to top
Application Notes This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 15 kDa in size corresponding to native human IL-2 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Comment

Gene Name: IL2

Restrictions For Research Use only

Handling

Product Details anti-IL2 Antibody Target Details IL2 Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

Images

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 2 (IL2) antibody (ABIN964758) anti-Human IL-2 antibody shows detection of a band ~15 kDa in size corresponding to r...
Western Blotting (WB) image for anti-Interleukin 2 (IL2) antibody (ABIN964758) anti-Human IL-2 antibody shows detection of a band ~15 kDa in size corresponding to r...