anti-Mouse (Murine) IL2 Receptor beta antibody for Flow Cytometry

Recommended IL2 Receptor beta Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, beta (IL2RB) Antibodies
  • IL2RB
  • il-2rb
  • CD122
  • IL15RB
  • P70-75
  • IL2RBC
  • IL-15Rbeta
  • IL15Rbeta
  • Il-2/15Rbeta
  • Il-2Rbeta
  • p70
  • interleukin 2 receptor subunit beta
  • interleukin-2 receptor subunit beta
  • interleukin 2 receptor, beta
  • interleukin 2 receptor, beta chain
  • IL2RB
  • LOC510185
  • il2rb
  • Il2rb
Reactivity
Mouse (Murine)
210
181
81
29
10
1
1
Host
Rat
143
120
68
Clonality
Monoclonal
Conjugate
This IL2 Receptor beta antibody is un-conjugated
28
28
20
14
6
5
4
4
4
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
180
128
66
62
41
26
15
14
12
9
8
4
4
3
2
1
1
1
1
Options
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Catalog No. ABIN135408
$ 302.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.810674 ABIN135409 FACS FITC Rat IgG2a Log in to see 5H4 2
15.810674 ABIN375796 FACS APC Rat IgG2a Log in to see 5H4 0
15.810674 ABIN376503 FACS PE Rat IgG2a Log in to see 5H4 0
15.810674 ABIN375945 FACS Biotin Rat IgG2a Log in to see 5H4 0
15.810674 ABIN376384 FACS Rat IgG2a Log in to see 5H4 0
14.5 ABIN1177313 BR FACS IP Rat IgG2b, kappa Log in to see TM-beta 1 18
14.5 ABIN2688932 FACS Biotin Rat IgG2b, kappa Log in to see TM 17
12.810674 ABIN288635 FACS FITC Rat IgG2a Log in to see 5H4 0
12.810674 ABIN288634 FACS IP Biotin Rat IgG2a Log in to see 5H4 0
12.810674 ABIN288633 FACS APC Rat IgG2a Log in to see 5H4 0
12.810674 ABIN284107 FACS IP Rat IgG2a Log in to see 5H4 0
12.810674 ABIN2135947 FACS IHC Rat IgG2b Log in to see TM-b1 0
12.810674 ABIN2135951 FACS PE Rat IgG2a kappa Log in to see 5H4 0
12.810674 ABIN2135955 FACS IHC Biotin Rat IgG2a kappa Log in to see 5H4 0
12.810674 ABIN2135949 FACS FITC Rat IgG2b Log in to see TM-b1 0
12.810674 ABIN2135953 FACS PE Rat IgG2b Log in to see TM-b1 0
12.810674 ABIN2135957 FACS Biotin Rat IgG2b Log in to see TM-b1 0
12.810674 ABIN284106 FACS IP Rat IgG2a Log in to see 5H4 0
9.792221 ABIN371155 FACS FITC Rat IgG2a Log in to see 5H4 4
9.792221 ABIN4900011 CyTOF FACS Rat IgG2a AA 27-240 Log in to see 130122 0

General

Antigen Interleukin 2 Receptor, beta (IL2RB) Antibodies
Reactivity Mouse (Murine)
(210), (181), (81), (29), (10), (1), (1)
Host Rat
(143), (120), (68)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL2 Receptor beta antibody is un-conjugated
(28), (28), (20), (14), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(180), (128), (66), (62), (41), (26), (15), (14), (12), (9), (8), (4), (4), (3), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL2 Receptor beta Antibody

Target Details IL2 Receptor beta Application Details Handling References for anti-IL2 Receptor beta antibody (ABIN135408) Images
Specificity Mouse CD122, 90-100 kDa
Cross-Reactivity (Details) Cross-reactivity with Stat1 from other sources has not been determined.
Predicted Reactivity A BLAST analysis was used to suggest cross-reactivity with Stat1 from mouse and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against Stat1 from human sources as 90% homology is noted.
Characteristics Rat Anti-Mouse CD122-UNLB
Purification Purified
Immunogen Rat myeloma YB2/0 transfected with truncated IL-2Rbeta cDNA (YB2/0-mbetat-28)
Clone 5H4
Isotype IgG2a
Plasmids, Primers & others

Target Details IL2 Receptor beta

Product Details anti-IL2 Receptor beta Antibody Application Details Handling References for anti-IL2 Receptor beta antibody (ABIN135408) Images back to top
Antigen
Alternative Name CD122 (IL2RB Antibody Abstract)
Background The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the chain which binds IL-2 with low affinity, (ii) the chain that binds IL-2 with high affinity, and (ii) the common chain (C) that does not bind IL-2. The high affinity receptor complex is an α/β/γ heterotrimer with a kDa of 1.3 x 10-11 M.2 In mouse spleen, CD122 is expresed on 30 % of CD8+ cells and all NK cells, but < 1 % of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells. Cytoplasmic regions of the IL-2R chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes.
Pathways JAK-STAT Signaling, Growth Factor Binding

Application Details

Product Details anti-IL2 Receptor beta Antibody Target Details IL2 Receptor beta Handling References for anti-IL2 Receptor beta antibody (ABIN135408) Images back to top
Application Notes
  • Applications: FC - Quality tested , IP - Reported in literature
  • Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and APC conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Sample Volume 1 mL
Restrictions For Research Use only

Handling

Product Details anti-IL2 Receptor beta Antibody Target Details IL2 Receptor beta Application Details References for anti-IL2 Receptor beta antibody (ABIN135408) Images back to top
Concentration 0.5 mg/mL
Buffer 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added
Preservative Without preservative
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Storage Comment Store at 2-8°C

References for anti-IL2 Receptor beta antibody (ABIN135408)

Product Details anti-IL2 Receptor beta Antibody Target Details IL2 Receptor beta Application Details Handling Images back to top
Background publications

Malek, Furse, Fleming, Fadell, He: "Biochemical identity and characterization of the mouse interleukin-2 receptor beta and gamma c subunits." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 15, Issue 5, pp. 447-54, 1995

Tanaka, Tsudo, Karasuyama, Kitamura, Kono, Hatakeyama, Taniguchi, Miyasaka: "A novel monoclonal antibody against murine IL-2 receptor beta-chain. Characterization of receptor expression in normal lymphoid cells and EL-4 cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 7, pp. 2222-8, 1991

Images

Product Details anti-IL2 Receptor beta Antibody Target Details IL2 Receptor beta Application Details Handling References for anti-IL2 Receptor beta antibody (ABIN135408) back to top
Supplier Images
 image for anti-Interleukin 2 Receptor, beta (IL2RB) antibody (ABIN135408) BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD112-FITC.