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|Antigen||Interleukin 4 Receptor (IL4R) Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Conjugate||This IL4 Receptor antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-IL4 Receptor AntibodyTarget Details IL4 Receptor Application Details Handling Images
|Specificity||This antibody is specific for Interleukin 4 Receptor, the transmembrane ligand-binding subunit of the heterodimeric IL-4 receptor complex. This antibody blocks cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/mL in the presence of 0.2 ng/mL of IL-4 using the factor dependant Human erythroleukemic cell line, TF-1.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Purification||Affinity chromatography on Protein A|
|Immunogen||Sf 21-derived, recombinant Human interleukin 4 Receptor, extracellular domain.|
Target Details IL4 ReceptorProduct Details anti-IL4 Receptor Antibody Application Details Handling Images back to top
|Alternative Name||CD124 / IL4R (IL4R Antibody Abstract)|
|Background||IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation. The IL4 response is involved in promoting Th2 differentiation. The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. It has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.Synonyms: 582J2.1, IL-4R-alpha, IL4 Receptor alpha, IL4RA, Interleukin-4 receptor alpha chain|
|Pathways||JAK-STAT Signaling, Positive Regulation of Immune Effector Process|
Application DetailsProduct Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Handling Images back to top
Flow Cytometry: 1/20 - 1/80. ELISA: 1/500 - 1/2000. Western Blot: 1/250 - 1/1000, Will detect human IL-4 R under non-reducing conditions. Functional Assays. Immunohistochemistry on frozen sections: 1/40 - 1/125.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Application Details Images back to top
|Buffer||PBS, 0.09 % Sodium Azide, 10 % Trehalose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|