anti-Mouse (Murine) IL4 Receptor antibody for Immunoprecipitation

Recommended IL4 Receptor Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 Receptor (IL4R) Antibodies
  • IL4R
  • il-4ra
  • zgc:113063
  • CD124
  • IL-4RA
  • IL4RA
  • Il4ra
  • IL4
  • Il4r
  • interleukin 4 receptor
  • interleukin 4 receptor, tandem duplicate 1
  • interleukin 4 receptor, alpha
  • IL4R
  • il4r.1
  • Il4r
  • Il4ra
Reactivity
Mouse (Murine)
109
50
11
Host
Rat
73
47
12
10
1
Clonality
Monoclonal
Conjugate
This IL4 Receptor antibody is un-conjugated
9
8
4
2
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Neutralization (Neut), ELISA
79
62
42
23
15
11
9
9
7
6
4
3
3
2
2
2
2
2
1
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Catalog No. ABIN2688940
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2688941 FACS IP Neut ELISA Rat IgG2a, kappa Log in to see mIL4R 8
1 ABIN2688938 FACS IP Neut ELISA Biotin Rat IgG2a, kappa Log in to see mIL4R 4
1 ABIN1177312 FACS IP Neut Rat IgG2a, kappa Log in to see mIL4R 8
1 ABIN2935520 IF/ICC IHC IP WB Rabbit AA 26-233 Log in to see Polyclonal 0

General

Antigen Interleukin 4 Receptor (IL4R) Antibodies
Reactivity Mouse (Murine)
(109), (50), (11)
Host Rat
(73), (47), (12), (10), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL4 Receptor antibody is un-conjugated
(9), (8), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Neutralization (Neut), ELISA
(79), (62), (42), (23), (15), (11), (9), (9), (7), (6), (4), (3), (3), (2), (2), (2), (2), (2), (1)
Pubmed 8 references available
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Product Details anti-IL4 Receptor Antibody

Target Details IL4 Receptor Application Details Handling References for anti-IL4 Receptor antibody (ABIN2688940) Images
Brand BD Pharmingen™
Characteristics The mIL4R-M2 monoclonal antibody specifically binds to the α subunit of the mouse Interleukin-4 Receptor (IL-4Rα, aka, CD124). The mIL4R-M2 antibody does not recognize human or rat IL-4R, crossreactivities with IL-4R from other species have not been established. The mouse IL-4Rα is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and nonhematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc, aka, type I IL-4R) or the IL-13 receptor alpha subunit (IL-4Rα/IL-13Rα, aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R binds and transduces signals from either IL-4 or IL-13. The mIL4R-M2 antibody blocks IL-4 binding to cells and is reported to be a potent inhibitor of IL-4's biological activities. The mIL4R-M2 antibody also recognizes naturally occurring, soluble truncated forms of IL-4Rα (sIL-4R) that result either from enzymatic cleavage of the cell surface extracellular IL-4Rα domain or from differential mRNA splicing and secretion by cells. These sIL-4R retain their high-affinity ligand binding domain and appear to either enhance or inhibit IL-4-mediated functions depending on the relative local levels of IL-4 and sIL-4R. The immunogen used to generate the mIL4R-M2 hybridoma was CTLL-19.4 cells, a subclone of CTLL-2 cells that express high levels of IL-4Rα.

BD Pharmingen™ Purified Rat Anti-Mouse CD124 - Purified - Clone mIL4R-M2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen CTLL-19.4 cells
Clone MIL4R
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details IL4 Receptor

Product Details anti-IL4 Receptor Antibody Application Details Handling References for anti-IL4 Receptor antibody (ABIN2688940) Images back to top
Antigen
Alternative Name CD124 (IL4R Antibody Abstract)
Background Synonyms: IL-4 Receptor α chain
Pathways JAK-STAT Signaling, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Handling References for anti-IL4 Receptor antibody (ABIN2688940) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Application Details References for anti-IL4 Receptor antibody (ABIN2688940) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-IL4 Receptor antibody (ABIN2688940)

Product Details anti-IL4 Receptor Antibody Target Details IL4 Receptor Application Details Handling Images back to top
Product cited in:

Kubo, Yamashita, Abe, Tada, Okumura, Ransom, Nakayama: "CD28 costimulation accelerates IL-4 receptor sensitivity and IL-4-mediated Th2 differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2432-42, 1999

Hassuneh, Nagarkatti, Nagarkatti: "Evidence for the participation of interleukin-2 (IL-2) and IL-4 in the regulation of autonomous growth and tumorigenesis of transformed cells of lymphoid origin." in: Blood, Vol. 89, Issue 2, pp. 610-20, 1997

Chilton, Fernandez-Botran: "Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5907-17, 1994

Sempowski, Beckmann, Derdak, Phipps: "Subsets of murine lung fibroblasts express membrane-bound and soluble IL-4 receptors. Role of IL-4 in enhancing fibroblast proliferation and collagen synthesis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3606-14, 1994

Feldman, Ruhl, Bickel, Finbloom, Pluznik: "Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6." in: Blood, Vol. 78, Issue 7, pp. 1678-84, 1991

Beckmann, Schooley, Gallis, Vanden Bos, Friend, Alpert, Raunio, Prickett, Baker, Park: "Monoclonal antibodies block murine IL-4 receptor function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 11, pp. 4212-7, 1990

Mosley, Beckmann, March, Idzerda, Gimpel, VandenBos, Friend, Alpert, Anderson, Jackson: "The murine interleukin-4 receptor: molecular cloning and characterization of secreted and membrane bound forms." in: Cell, Vol. 59, Issue 2, pp. 335-48, 1989

Lowenthal, Castle, Christiansen, Schreurs, Rennick, Arai, Hoy, Takebe, Howard: "Expression of high affinity receptors for murine interleukin 4 (BSF-1) on hemopoietic and nonhemopoietic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 2, pp. 456-64, 1988