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|Antigen||Interleukin 5 (IL5) Antibodies|
|Reactivity||Guinea Pig, Human, Mouse (Murine) Alternatives|
|Conjugate||This IL5 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-IL5 AntibodyTarget Details IL5 Application Details Handling References for anti-IL5 antibody (ABIN118519) Images
|Specificity||This antibody is specific for Interleukin 5 (IL-5). Clone TRFK5 may be used to neutralise IL-5 bioactivity in a functional assay (1,2).|
|Purification||Affinity chromatography on Protein G|
|Immunogen||Purified natural murine IL-5.|
Target Details IL5Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 antibody (ABIN118519) Images back to top
|Alternative Name||Interleukin-5 / IL5 (IL5 Antibody Abstract)|
|Background||IL-5 is a T cell derived haematopoietic growth factor, which stimulates eosinophil differentiation and activation.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF|
|Pathways||JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour|
Application DetailsProduct Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 antibody (ABIN118519) Images back to top
Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: This antibody may be used in combination with clone TRFK4 (ABIN181863) in ELISA and ELISPOT assays. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 antibody (ABIN118519) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-IL5 antibody (ABIN118519)Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Akdis, Akdis, Blesken, Wymann, Alkan, Mueller, Blaser: "Epitope-specific T cell tolerance to phospholipase A2 in bee venom immunotherapy and recovery by IL-2 and IL-15 in vitro." in: The Journal of clinical investigation, Vol. 98, Issue 7, pp. 1676-83, 1996
Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992
Betz, Fox: "Regulation and development of cytochrome c-specific IL-4-producing T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 4, pp. 1046-52, 1990
Schumacher, OGarra, Shrader, van Kimmenade, Bond, Mosmann, Coffman: "The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 5, pp. 1576-81, 1988