General
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Antigen
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Interleukin 6 Receptor, alpha (Il6ra) Antibodies
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Epitope
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Internal Region
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Reactivity
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Mouse (Murine)
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Host
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Goat
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Clonality
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Conjugate
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This IL6RA antibody is un-conjugated
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Application
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ELISA, Western Blotting (WB)
Alternatives
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Supplier
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Log in to see
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Specificity
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This antibody is expected to recognize all reported isoforms (NP_000556.1, NP_852004.1). The sequence of the immunizing peptide corresponds with a part of the extracellular domain.
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Purification
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Il6ra Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
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Il6ra Antibody was raised against synthetic peptide YLLQFQLRYRPVWSK found at an internal region of Il6ra.
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Antigen
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Alternative Name
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Il6ra (Il6ra Antibody Abstract)
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Gene ID
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16194
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NCBI Accession
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NP_034689
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UniProt
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P22272
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Pathways
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JAK-STAT Signaling, Autophagy, Growth Factor Binding
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Application Notes
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ELISA: antibody detection limit dilution 1:2000. Western Blot: Preliminary experiments in Mouse Spleen and Thymus lysates and in lysates of cell lines NIH3T3 and NSO gave no specific signal but low background (at antibody concentration up to 1μ,g/mL).
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Restrictions
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For Research Use only
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Format
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Liquid
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Concentration
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500 μg/mL
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Buffer
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Il6ra is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
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Sodium azide
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Precaution of Use
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This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
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-20 °C
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Storage Comment
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Aliquot and store at -20°C. Minimize freezing and thawing.
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