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|Antigen||Interleukin 7 (IL7) Antibodies|
|Conjugate||This IL7 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
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Product Details anti-IL7 AntibodyTarget Details IL7 Application Details Handling Images
|Specificity||This antibody recognises Human IL-7.|
|Purification||Affinity Chromatography on Protein A.|
|Immunogen||Recombinant human IL-7.|
Target Details IL7Product Details anti-IL7 Antibody Application Details Handling Images back to top
|Alternative Name||Interleukin-7 / IL7 (IL7 Antibody Abstract)|
|Background||Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.Synonyms: IL-7|
Application DetailsProduct Details anti-IL7 Antibody Target Details IL7 Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-7 (0.5 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA: In a Sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 1000 pg/mL of recombinant Human IL-7 when used withBuiotin anti-Human IL-7 antibody (PP1029B1 or PP1029B2) as the detection antibody at aconcentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Hunman IL-7 by Western Blot analysis this antibody can be used ata concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human IL-7 is 2.0-4.0 ng/lane, under reducingconditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL7 Antibody Target Details IL7 Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 1.0 mg/mL.|
|Buffer||PBS without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|