anti-Human IL7R antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended IL7R Antibody (supplied by: Log in to see )

Antigen
Interleukin 7 Receptor (IL7R) Antibodies
  • IL7R
  • CD127
  • IL-7Ralpha
  • CDW127
  • IL-7R-alpha
  • IL7RA
  • ILRA
  • IL-7RA
  • interleukin 7 receptor
  • IL7R
  • Il7r
Epitope
pTyr449
28
27
15
15
13
8
8
7
7
7
6
5
5
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
236
207
47
5
2
2
1
Host
Rabbit
143
138
92
17
7
4
1
Clonality
Polyclonal
Conjugate
This IL7R antibody is un-conjugated
30
28
24
19
16
14
12
8
7
7
7
6
6
5
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
180
175
81
72
44
26
20
12
12
11
9
8
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN351553
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.116877 ABIN351552 ELISA IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
7 ABIN801438 IF (p) IHC (p) WB Rabbit IgG AA 440-451, pTyr449 Log in to see Polyclonal 0
4 ABIN372989 EIA IHC (p) WB Rabbit pTyr449 Log in to see Polyclonal 0
4 ABIN731063 IF (p) IHC (p) WB Rabbit IgG AA 250-300 Log in to see Polyclonal 0
1 ABIN801447 IHC (p) WB HRP Rabbit IgG AA 440-451, pTyr449 Log in to see Polyclonal 0
1 ABIN731072 IHC (p) WB HRP Rabbit IgG AA 250-300 Log in to see Polyclonal 0
1 ABIN801440 IHC (p) WB Biotin Rabbit IgG AA 440-451, pTyr449 Log in to see Polyclonal 0
1 ABIN731065 IHC (p) WB Biotin Rabbit IgG AA 250-300 Log in to see Polyclonal 0
1 ABIN5561030 IHC (p) WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Interleukin 7 Receptor (IL7R) Antibodies
Epitope pTyr449
(28), (27), (15), (15), (13), (8), (8), (7), (7), (7), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(236), (207), (47), (5), (2), (2), (1)
Host Rabbit
(143), (138), (92), (17), (7), (4), (1)
Clonality
Polyclonal
Conjugate This IL7R antibody is un-conjugated
(30), (28), (24), (19), (16), (14), (12), (8), (7), (7), (7), (6), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(180), (175), (81), (72), (44), (26), (20), (12), (12), (11), (9), (8), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-IL7R Antibody

Target Details IL7R Application Details Handling Images
Brand IHC-plus™
Specificity A BLAST analysis was used to suggest cross-reactivity with IL-7R from human, mouse and rat sources based on 100 % homology with the immunizing sequence. Cross-reactivity with IL-7R from other sources has not been determined.
Purification Immunoaffinity purified
Immunogen Synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details IL7R

Product Details anti-IL7R Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL7R / CD127 (IL7R Antibody Abstract)
Background Name/Gene ID: IL7R
Family: Interleukin

Synonyms: IL7R, CD127, CD127 antigen, IL-7RA, IL-7 receptor subunit alpha, IL7RA, ILRA, Interleukin 7 receptor, CDW127, IL-7R subunit alpha, IL-7R-alpha
Gene ID 3575
UniProt P16871
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size

Application Details

Product Details anti-IL7R Antibody Target Details IL7R Handling Images back to top
Application Notes Approved: ELISA (1:100000), IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:10000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Expect a band approximately 55 kD in size corresponding to phosphorylated IL-7 in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Comment

Target Species of Antibody: Mouse

Restrictions For Research Use only

Handling

Product Details anti-IL7R Antibody Target Details IL7R Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C,-20 °C
Storage Comment 4°C or -20°C, Avoid freeze-thaw cycles.

Images

Product Details anti-IL7R Antibody Target Details IL7R Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody (ABIN351553) Human Colon, MALT (formalin-fixed, paraffin-embedded) stained with IL7R antibody ABIN...
Immunohistochemistry (IHC) image for anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody (ABIN351553) Anti-IL-7 Receptor antibody IHC of human colon, MALT. Immunohistochemistry of formali...