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|Antigen||Interleukin 9 Receptor (IL9R) Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Conjugate||This IL9 Receptor antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Neutralization (Neut), ELISA
|Supplier||Log in to see|
Product Details anti-IL9 Receptor AntibodyTarget Details IL9 Receptor Application Details Handling Images
|Specificity||Recognizes human IL-9R. In direct ELISAs, this antibody does not cross-react with rhIL-4 R, rhIL-5 R, rhIL-5 R, rhIL-13 R 1, rhIL-13 R 2, or rmIL-9 R.|
|Purification||Protein G purified|
Sf21-derived rhIL-9 R extracellular domain.
Type of Immunogen: Recombinant protein
Target Details IL9 ReceptorProduct Details anti-IL9 Receptor Antibody Application Details Handling Images back to top
|Alternative Name||IL9R / CD129 (IL9R Antibody Abstract)|
Name/Gene ID: IL9R
Synonyms: IL9R, IL-9 receptor, IL-9R, Interleukin 9 receptor, Interleukin-9 receptor, CD129, CD129 antigen
|Pathways||JAK-STAT Signaling, Growth Factor Binding|
Application DetailsProduct Details anti-IL9 Receptor Antibody Target Details IL9 Receptor Handling Images back to top
Approved: ELISA, Flo, Func, Neut
Usage: Neutralization of bioactivity, Flow Cytometry, Direct ELISA. Neutralization of Human IL-9 Receptor-mediated bioactivity: The exact concentration of antibody required to neutralize the human cell surface IL-9 R mediated bioactivity is dependent on the IL-2 concentration as well as on the number and types of IL-2 receptors present on the cell surface (a function of cell type and culture conditions). The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-9 R mediated IL-9 response on a responsive cell line, at a specific IL-9 concentration. The ND50 for this lot of anti-human IL-9 R antibody was determined to be approximately 2-4 μg/mL for inhibiting the IL-9-dependent proliferation of MO7e cells (1 x 10^5 cells/mL) in the presence of 2 ng/mL of rhIL-9. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human IL-9 R. The detection limit for rhIL-9 R is approximately 6 ng/well.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL9 Receptor Antibody Target Details IL9 Receptor Application Details Images back to top
|Reconstitution||Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.|
|Buffer||Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.|